[1]丁丽华),于 斐),张冠军),等.脱氧雪腐镰刀菌烯醇的高灵敏化学发光磁酶免疫法检测*[J].郑州大学学报(医学版),2017,(02):134-137.[doi:10.13705/j.issn.1671-6825.2017.02.007]
 DING Lihua),YU Fei),ZHANG Guanjun),et al.Determination of deoxynivalenol by a sensitive method of magnetic enzyme chemiluminescence immunoassay[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(02):134-137.[doi:10.13705/j.issn.1671-6825.2017.02.007]
点击复制

脱氧雪腐镰刀菌烯醇的高灵敏化学发光磁酶免疫法检测*()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年02期
页码:
134-137
栏目:
论著
出版日期:
2017-03-15

文章信息/Info

Title:
Determination of deoxynivalenol by a sensitive method of magnetic enzyme chemiluminescence immunoassay
作者:
丁丽华1)于 斐2)张冠军1)熊亚敏1)刘利娥2)#吴拥军2)玉崧成2)
1)郑州大学公共卫生学院营养与食品卫生学教研室 郑州 450001
2)郑州大学公共卫生学院卫生化学教研室 郑州 450001
Author(s):
DING Lihua1) YU Fei2) ZHANG Guanjun1) XIONG Yamin1) LIU Li'e2) WU Yongjun2) YU Songcheng2)
1)Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, Zhengzhou 450001
2)Department of Sanitary Chemistry,College of Public Health,Zhengzhou University,Zhengzhou 450001
关键词:
脱氧雪腐镰刀菌烯醇 金磁复合微粒 化学发光磁酶免疫法
Keywords:
deoxynivalenol gold magnetic partical magnetic enzyme chemiluminescence immunoassay
分类号:
R155.5+2
DOI:
10.13705/j.issn.1671-6825.2017.02.007
摘要:
目的:建立一种快速简便、灵敏度高的新型化学发光磁酶免疫法(MECLIA)检测脱氧雪腐镰刀菌烯醇(DON)。方法:制备Fe3O4@SiO2@Au金磁复合微粒及DON与卵清蛋白(OVA)的复合物(OVA-DON); 以Fe3O4@SiO2@Au为固相载体,优化条件,使其表面负载OVA-DON形成DON金磁免疫复合微粒; 以鲁米诺-过氧化氢-辣根过氧化物酶-苯酚类化合物作为增强化学发光体系,建立MECLIA对小麦中DON进行检测,并对所建立的方法进行方法学评价。结果:该方法标准曲线方程为Y=-521 951X+2 883 979(X为DON质量浓度的对数,Y为化学发光值,R2=0.998 6),线性范围为0.10~20.00 μg/L,检测限为31 ng/L; 批内RSD为2.2%~3.5%,批间RSD为4.9%~9.6%,标准样品回收率为95.0%~108.0%,小麦样品回收率为92.0%~106.0%。结论:该研究建立的MECLIA灵敏度高、精密度好、操作简单,可用于大批量小麦样品中DON的快速检测。
Abstract:
Aim: To propose a magnetic enzyme chemiluminescence immunoassay(MECLIA)for rapid and sensitive detection of deoxynivalenol(DON).Methods: Fe3O4@SiO2@Au particles and the conjugation of DON to ovalbumin(OVA-DON)were prepared.Then,the DON-OVA was coated on the surface of Fe3O4@SiO2@Au by using Fe3O4@SiO2@Au as immobile phase. After that, MECLIA based on the luminol-H2O2-HRP-phenols system for detection of DON was established. The accuracy and sensitivity of the established method were evaluated.Results: The standard curve equation was Y=-521 951X+2 883 979(X was the logarithm of the concentration of DON,Y was the illumination value of the standard concentration, R2=0.998 6), the linear range was 0.10~20.00 μg/L, the limit of detection was 31 ng/L, the relative standard deviations were less than 3.5% and 9.6% for intra- and inter-assay, respectively, and the recoveries ranged from 95.0% to 108.0%. The proposed method was satisfactorily applied to determine DON in wheat sample and the recoveries ranged from 92.0% to 106.0%.Conclusion: The developed MECLIA is sensitive and simple with good precision for DON residue analysis in wheat samples.

参考文献/References:

[1] WU F,GROOPMAN JD,PESTKA JJ.Public health impacts of foodborne mycotoxins[J].Annu Rev Food Sci Technol,2014,5:351
[2] YU Q, LI H, LI CL, et al. Gold nanoparticles-based lateral flow immunoassay with silver staining for simultaneous detection of fumonisin B1 and deoxynivalenol[J].Food Control, 2015,54:347
[3] SHAR ZH,SUMBAL GA,SHERAZI STH,et al.Determination of deoxynivalenol in poultry feed by three gas chromatographic detection techniques[J].Chromatographia,2014,77(3/4):337
[4] 张玉梅,袁俊勇,朱国琴,等.全血快速检测甲胎蛋白胶体金免疫层析方法的研制和应用[J].郑州大学学报(医学版),2004,39(6):1037
[5] 王华栋,于斐,刘向辉,等.高效液相色谱法同时测定膀胱镜胶中利多卡因与羟苯乙酯的含量[J].郑州大学学报(医学版),2014,49(4):526
[6] 张琳,李玲,孔海瑞,等.基于气相色谱质谱技术的肾细胞癌患者尿液代谢组学分析[J].南方医科大学学报,2015,35(5):763
[7] YU SC,YU F,LI YP,et al.Magnetic nanoparticles replacing microplate as immobile phase could greatly improve the sensitivity of chemiluminescence enzymatic immunoassay for deoxynivalenol[J].Food Control,2016,60:500
[8] CHEN LN,ZENG BR,WU YG,et al.Gold-loading magnetic core-shell organic/inorganic nanocomposites:facile preparation and multiple properties[J].Polym Advan Technol,2014,25(9):1069
[9] WANG AJ,LI YF,LI ZH,et al.Amperometric glucose sensor based on enhanced catalytic reduction of oxygen using glucose oxidase adsorbed onto core-shell Fe3O4@silica@Au magnetic nanoparticles[J].Mater Sci Eng C Mater Biol Appl,2012,32(6):1640
[10]MARAGOS CM,MCCORMICK SP.Monoclonal antibodies for the mycotoxins deoxynivalenol and 3-acetyl-deoxynivalenol[J].Food Agr Immunol,2000,12(3):181
[11]LI YS,LIU GZ,FU XJ,et al.High-sensitive chemiluminescent ELISA method investigation for the determination of deoxynivalenol in rice[J].Food Anal Methods,2015,18(3):656
[12]GAO L,ZHUANG J,NIE L,et al.Intrinsic peroxidase-like activity of ferromagnetic nanoparticles[J].Nat Nanotechnol,2007,2(9):577

备注/Memo

备注/Memo:
*国家自然科学基金青年项目 81402721; 河南省教育厅自然科学基础研究项目 14A330008; 2014郑州大学研究生教育科研专项基金资助项目
#通信作者,女,1966年12月生,博士,教授,研究方向:食物营养与新型分析技术,E-mail:zzdxlle66@zzu.edu.cn
更新日期/Last Update: 2017-03-20