[1]孟 泳,崔应麟#,李 彬.厚朴麻黄汤对哮喘小鼠血清IgE、IL-4、IL-13及半胱氨酰白三烯水平的影响*[J].郑州大学学报(医学版),2017,(02):193-196.[doi:10.13705/j.issn.1671-6825.2017.02.022]
 MENG Yong,CUI Yinglin,LI Bin.Effects of Houpu Mahuang Decoction on serum levels of IgE, IL-4, IL-13 and cysteinyl leukotrienes in asthmatic mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(02):193-196.[doi:10.13705/j.issn.1671-6825.2017.02.022]
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厚朴麻黄汤对哮喘小鼠血清IgE、IL-4、IL-13及半胱氨酰白三烯水平的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年02期
页码:
193-196
栏目:
应用研究
出版日期:
2017-03-15

文章信息/Info

Title:
Effects of Houpu Mahuang Decoction on serum levels of IgE, IL-4, IL-13 and cysteinyl leukotrienes in asthmatic mice
作者:
孟 泳崔应麟#李 彬
河南省中医院呼吸科 郑州 450002
Author(s):
MENG YongCUI YinglinLI Bin
Department of Respiratory Medicine, Henan Traditional Chinese Medicine Hospital,Zhengzhou 450002
关键词:
支气管哮喘 厚朴麻黄汤 IgE IL-4 IL-13 CysLTs 小鼠
Keywords:
bronchial asthma Houpu Mahuang Decoction IgE IL-4 IL-13 CysLTs mouse
分类号:
R562.25
DOI:
10.13705/j.issn.1671-6825.2017.02.022
摘要:
目的:观察厚朴麻黄汤对哮喘小鼠肺组织病理学改变及血清IgE、IL-4、IL-13、半胱氨酰白三烯(CysLTs)水平的影响。方法:60只小鼠分为空白组、模型组、西药组、中药低剂量组、中药中剂量组和中药高剂量组,每组10只。制备哮喘小鼠模型,中药各治疗组分别给予厚朴麻黄汤(16、32、64 g/kg)进行干预。西药组给予地塞米松(0.3 mg/kg)进行干预。实验结束后,光镜下观察各组小鼠肺组织病理学改变,ELISA法检测血清IgE、IL-4、IL-13及CysLTs的水平。结果:中药各治疗组可不同程度地减轻哮喘小鼠的肺组织病理学改变。与模型组相比,中药低、中、高剂量组血清IgE、IL-4、IL-13及CysLTs水平均降低(P均<0.05); 与西药组相比,中药低、中剂量组上述各指标均升高(P均<0.05)。结论:厚朴麻黄汤能够减轻哮喘小鼠肺组织病理学改变,降低血清IgE、IL-4、IL-13及CysLTs水平,抑制炎症因子产生,从而减轻气道炎症。
Abstract:
Aim: To observe the effects of Houpu Mahuang Decoction on asthmatic mice's lung pathological changes and serum IgE, IL-4, IL-13, cysteinyl leukotrienes(CysLTs)levels.Methods: A total of 60 mice were allocated into control group,model group,western medicine group,low dose Chinese medicine group,middle dose Chinese medicine group,and high dose Chinese medicine group. The mice model of asthma was prepared. The mice in Chinese medicine groups were given Houpu Mahuang Decoction at high, middle and low doses(16, 32, 64 g/kg), and those in western medicine group was given dexamethasone solution(0.3 mg/kg).The pathological changes of the lung tissue of the mice were observed. The serum IgE, IL-4, IL-13 and CysLTs levels were determined by ELISA.Results: The pathological changes of lung tissue of mice in 3 Chinese medicine groups improved in different degrees. Compared with the model group, serum IgE, IL-4, IL-13 and CysLTs levels of the Chinese medicine groups significantly decreased(P<0.05). Compared with the western medicine group, the above indexes of the low and middle Chinese medicine groups significantly increased(P<0.05).Conclusion: Houpu Mahuang Decoction could alleviate the pathological changes in lung tissue of asthmatic mice, reduce serum IgE, IL-4, IL-13 and CysLTs, inhibit inflammatory cytokine production, thereby reducing the airway inflammation.

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备注/Memo

备注/Memo:
*河南省重点科技攻关计划项目 152102310107
#通信作者,男,1963年10月生,硕士,主任医师,教授,研究方向:内科疾病的中医药防治,E-mail:mywd1966@sina.com
更新日期/Last Update: 2017-03-20