[1]张青青),张 苗),尚彩红),等.2-甲氧基雌二醇及其合成中间体的大鼠肺细胞膜色谱行为[J].郑州大学学报(医学版),2017,(02):213-216.[doi:10.13705/j.issn.1671-6825.2017.02.027]
 ZHANG Qingqing),ZHANG Miao),SHANG Caihong),et al.Establishment of rat lung cell membrane chromatography and chromatographic affinity of 2-methoxyestradiol and synthetic derivatives[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(02):213-216.[doi:10.13705/j.issn.1671-6825.2017.02.027]
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2-甲氧基雌二醇及其合成中间体的大鼠肺细胞膜色谱行为()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年02期
页码:
213-216
栏目:
应用研究
出版日期:
2017-03-15

文章信息/Info

Title:
Establishment of rat lung cell membrane chromatography and chromatographic affinity of 2-methoxyestradiol and synthetic derivatives
作者:
张青青1)张 苗2)尚彩红1)周建华1)郭丽芳1)杜 斌3)#
1)郑州大学第二附属医院药剂科 郑州 450014
2)沈阳药科大学药学院药物制剂教研室 沈阳 110016
3)郑州大学药学院药物分析教研室 郑州 450001
Author(s):
ZHANG Qingqing1) ZHANG Miao2) SHANG Caihong1) ZHOU Jianhua1) GUO Lifang1) DU Bin3)
1)Department of Pharmacy, the Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014
2)Department of Pharmaceutical Preparation School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016
3)Department of Pharmaceutical Analysis,School of Pharmacy, Zhengzhou University, Zhengzhou 450001
关键词:
2-甲氧基雌二醇 细胞膜色谱 靶向性
Keywords:
2-methoxyestradiol cell membrane chromatography targeting
分类号:
R979.1
DOI:
10.13705/j.issn.1671-6825.2017.02.027
摘要:
目的:探讨实验室合成的2-甲氧基雌二醇(2-ME)及其合成中间体的大鼠肺细胞膜色谱行为。方法:经筛选,大鼠细胞膜色谱条件为自制大鼠肺细胞膜色谱柱(10 mm×2 mm),流动相为10 mmol/L磷酸盐缓冲液(pH 7.4),柱温为37 ℃,流速为0.2 mL/min。以沙丁胺醇为阳性对照,以容量因子k'作为指标,考察2-ME及其合成中间体在大鼠肺细胞膜色谱柱上的保留特性。结果:2-ME及合成中间体1、2号化合物在大鼠肺细胞膜色谱柱上均有吸附,且k'依次为2号化合物>1号化合物>2-ME; 合成中间体3及4号化合物未表现出吸附特性。2-ME与大鼠肺细胞膜色谱柱的亲和力大于纯硅胶色谱柱、大鼠心肌细胞膜色谱柱和肝细胞膜色谱柱。结论:2-ME在大鼠肺细胞膜上具有特异性亲和。
Abstract:
Aim: To screen the activity of 2-methoxyestradiol(2-ME)and synthetic derivatives by rat lung cell membrane chromatography.Methods: The lung cell membrane stationary phase(CMSP)was packed into a stainless steel column(10 mm×2 mm), and the HPLC conditions were as follows:a mobile phase consisted of 10 mmol/L phosphate buffer(pH 7.4)at a flow rate of 0.2 mL/min, and the column temperature was 37 ℃. Salbutamol was used as positive control in the screening. The capacity factor k' as chromatographic parameters was used to describe the affinity of 2-ME and its synthetic derivatives.Results: 2-ME,NO.1 synthetic derivative and NO.2 synthetic derivative had chromatographic retention on lung CMSP column and k' of NO.2 was greater than NO.1 and 2-ME. The k' of 2-ME with rat lung CMSP was higher than those with pure silica gel column,rat cardiac muscle CMSP and liver CMSP.Conclusion: 2-ME has the property of specific affinity on the membrane of the rat lung cell membrane.

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备注/Memo

备注/Memo:
#通信作者,女,1968年8月生,博士,教授,硕士研究生导师,研究方向:抗肿瘤药物的研发,E-mail:dubin@zzu.edu.cn
更新日期/Last Update: 2017-03-20