[1]王鹏举),彭有梅),张 艳),等.余甘根苷B对柯萨奇病毒B3感染的心肌细胞及Balb/c小鼠的保护作用*[J].郑州大学学报(医学版),2017,(03):263-267.[doi:10.13705/j.issn.1671-6825.2017.03.006]
 WANG Pengju),PENG Youmei),ZHANG Yan),et al.Protection effect of phyllaemblicin B on coxsackie virus B3 infected myocardiocytes and Balb/c mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(03):263-267.[doi:10.13705/j.issn.1671-6825.2017.03.006]
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余甘根苷B对柯萨奇病毒B3感染的心肌细胞及Balb/c小鼠的保护作用*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年03期
页码:
263-267
栏目:
论著
出版日期:
2017-05-15

文章信息/Info

Title:
Protection effect of phyllaemblicin B on coxsackie virus B3 infected myocardiocytes and Balb/c mice
作者:
王鹏举1)彭有梅2)张 艳2)江金花2)范程程3)娄晓月3)陈文娟3)王亚峰3)#
1)郑州大学基础医学院中英分子肿瘤学研究中心 郑州 450052 2)河南省(郑州大学)医药科学研究院河南省肝病药理重点实验室 郑州 450052 3)郑州大学药学院临床药理学系 郑州 450001
Author(s):
WANG Pengju1) PENG Youmei2) ZHANG Yan2) JIANG Jinhua2) FAN Chengcheng3) LOU Xiaoyue3) CHEN Wenjuan3) WANG Yafeng3)
1)Sino-British Research Centre for Molecular Oncology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052 2)Henan Key Laboratory for Pharmacology of Liver Diseases, Henan Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450052 3)Department of Clinical pharmacology, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001
关键词:
余甘根苷B 柯萨奇病毒B3 心肌细胞
Keywords:
phyllaemblicin B coxsackie virus B3 myocardiocyte
分类号:
R285.5
DOI:
10.13705/j.issn.1671-6825.2017.03.006
摘要:
目的:探讨余甘根苷B对柯萨奇病毒B3(CVB3)感染的心肌细胞及Balb/c小鼠的保护作用。方法:首先构建CVB3感染的心肌细胞模型和动物模型,观察心肌细胞的病变效应,检测心肌细胞上清液中天冬氨酸氨基转移酶(AST)和乳酸脱氢酶(LDH)释放量的变化,并通过电子显微镜观察小鼠心肌组织的超微结构变化,检测余甘根苷B对心肌细胞的保护作用。结果:在CVB3感染的心肌细胞模型中,CVB3感染可引起细胞发生碎裂、脱落等细胞病变现象,余甘根苷B作用后,可以显著减轻细胞病变,同时能明显抑制细胞释放的心肌酶AST和LDH的活性(P<0.05); 在CVB3感染的小鼠模型中,小鼠心肌组织超微结构的结果显示,CVB3感染能引起心肌纤维断裂溶解、细胞核结构破坏等病理损伤,余甘根苷B 则能减轻CVB3感染造成的心肌组织的损伤。结论:余甘根苷B对CVB3感染的心肌细胞和Balb/c小鼠具有明显的保护作用。
Abstract:
Aim: To investigate the protection effect of phyllaemblicin B on myocardiocytes and Balb/c mice infected with coxsackie virus B3(CVB3).Methods: Viral myocarditis(VMC)models infected with CVB3 were established in myocardiocytes and Balb/c mice. Cell survival rate was measured by cytopathic effect(CPE)assay, the contents of aspartate transferase(AST)and lactate dehydrogenase(LDH)were determined in supernatant of myocardiocytes culture medium. Histopathological changes of myocardium of Balb/c mice infected with CVB3 were analyzed to test the antiviral activity of phyllaemblicin B.Results: CVB3 infection induced obvious CPE in myocardiocytes in vitro. After treatment with phyllaemblicin B, CPE on myocardiocytes infected with CVB3 was obviously inhibited, meanwhile the activities of AST and LDH were dramatically reduced compare with the untreatment group(P<0.05). Ultra-structure of mouse myocardium demonstrated that CVB3 infection led to pathological lesions including cardiac muscle fibers breakdown and destruction of nucleus structure. With the treatment of phyllaemblicin B, the histopathology changes in mice myocardium were alleviated.Conclusion: Phyllaemblicin B has obvious protection effect on myocardiocytes and Balb/c mice infected with CVB3.

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备注/Memo

备注/Memo:
*国家自然科学基金青年科学基金项目 81302813
#通信作者,女,1980年5月生,博士,讲师,研究方向:抗病毒药理,E-mail:yfwang@zzu.edu.cn
更新日期/Last Update: 2017-05-20