[1]罗真真),张 震),乔亚敏),等.萝卜硫素对结直肠癌SW620细胞增殖、凋亡及迁移的影响*[J].郑州大学学报(医学版),2017,(03):281-284.[doi:10.13705/j.issn.1671-6825.2017.03.010]
 LUO Zhenzhen),ZHANG Zhen),QIAO Yamin),et al.Effects of sulforaphane on proliferation,apoptosis and migration of SW620 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(03):281-284.[doi:10.13705/j.issn.1671-6825.2017.03.010]
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萝卜硫素对结直肠癌SW620细胞增殖、凋亡及迁移的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年03期
页码:
281-284
栏目:
论著
出版日期:
2017-05-15

文章信息/Info

Title:
Effects of sulforaphane on proliferation,apoptosis and migration of SW620 cells
作者:
罗真真123)张 震123)乔亚敏123)陈新峰123)黄 岚123)张 毅1234)#
1)郑州大学第一附属医院肿瘤科 郑州 450052 2)郑州大学第一附属医院生物治疗中心 郑州 450052 3)河南省肿瘤免疫治疗工程技术研究中心 郑州 450052 4)郑州大学生命科学学院 郑州 450001
Author(s):
LUO Zhenzhen123)ZHANG Zhen123)QIAO Yamin123)CHEN Xinfeng123)HUANG Lan123)ZHANG Yi1234)
1)Department of Oncology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Bio-therapy Center, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 3)Engineering and Technology Research Center for Tumor Immunotherapy of Henan Province, Zhengzhou 450052 4)School of Life Sciences, Zhengzhou University, Zhengzhou 450001
关键词:
萝卜硫素 结直肠癌 SW620细胞 STAT3 NF-κB MMP
Keywords:
sulforaphane colorectal cancer SW620 cell STAT3 NF-κB MMP
分类号:
R735.3
DOI:
10.13705/j.issn.1671-6825.2017.03.010
摘要:
目的:观察萝卜硫素(SFN)对结直肠癌SW620细胞增殖、凋亡及迁移的影响,并探讨其可能的作用机制。方法:采用CCK-8法检测0、5、10、15、20 μmol/L SFN处理48、72 h对SW620细胞增殖能力的影响,采用流式细胞术检测0、5、10、15、20 μmol/L SFN处理24、48 h对SW620细胞凋亡的影响,采用细胞划痕实验观察20 μmol/L SFN处理24 h对SW620细胞迁移的影响,并采用Western blot法检测细胞中STAT3、NF-κB、p-STAT3、p-NF-κB及MMP-2、MMP-9蛋白的表达。结果:随着SFN浓度的升高,SW620细胞增殖受到抑制,细胞凋亡率升高(P<0.05)。同时20 μmol/L SFN可明显减弱SW620细胞的迁移能力,降低p-STAT3、p-NF-κB与MMP-2及MMP-9蛋白的表达(P<0.05)。结论:SFN抑制SW620细胞增殖、促进其凋亡的机制可能与降低p-STAT3、p-NF-κB的表达有关,抑制侵袭及转移的机制可能与其降低MMP-2及MMP-9蛋白的表达有关。
Abstract:
Aim: To observe the effects of sulforaphane(SFN)on proliferation, apoptosis, and migration of SW620 cells in vitro and to explore the potential mechanism involved.Methods: SW620 cells were treated with 0,5,10,15,20 μmol/L SFN for 48,72 h and the cell proliferation was detected by CCK-8 kit. SW620 cells were treated with 0,5,10,15,20 μmol/L SFN for 24,48 h and the cell apoptosis was detected by flow cytometry. SW620 cells were treated with 20 μmol/L SFN for 24 h, the migration capability was measured by the wound healing test, and the protein expression levels of STAT3, p-STAT3, NF-κB, p-NF-κB, MMP-2 and MMP-9 were determined by Western blot.Results: SFN significantly reduced the proliferation and increased apoptosis in a dose-dependent manner(P<0.05). The ability of migration was significantly inhibited by 20 μmol/L SFN(P<0.05),and the expressions of p-STAT3, p-NF-κB, MMP-2, and MMP-9 protein were significantly down-regulated(P<0.05).Conclusion: It is indicated that SFN could inhibit the proliferation and the ability of migration, and induce apoptosis of SW620 cells, which may be related with its down-regulating the expressions of p-STAT3, p-NF-κB, MMP-2 and MMP-9 protein.

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备注/Memo

备注/Memo:
*河南省科技厅基础与前沿技术研究基金 112300410153,122300410155
#通信作者,男,1964年4月生,博士,教授,主任医师,研究方向:肿瘤免疫学、肿瘤干细胞和生物细胞治疗,E-mail:yizhang@zzu.edu.cn
更新日期/Last Update: 2017-05-20