[1]袁恩武)△,王银芳),邢金芳),等.妊娠早期稽留流产患者绒毛组织中HIF-1α、BNIP3、LC3的表达*[J].郑州大学学报(医学版),2017,(03):335-339.[doi:10.13705/j.issn.1671-6825.2017.03.023]
 YUAN Enwu),WANG Yinfang),XING Jinfang),et al.Expressions of HIF-1α,BNIP3,LC3 in villi from with women early pregnancy missed abortion[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(03):335-339.[doi:10.13705/j.issn.1671-6825.2017.03.023]
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妊娠早期稽留流产患者绒毛组织中HIF-1α、BNIP3、LC3的表达*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年03期
页码:
335-339
栏目:
应用研究
出版日期:
2017-05-15

文章信息/Info

Title:
Expressions of HIF-1α,BNIP3,LC3 in villi from with women early pregnancy missed abortion
作者:
袁恩武1)△王银芳1)邢金芳1)闫广伟1)孙利环2)代延朋1)
1)郑州大学第三附属医院检验科 郑州 450052 2)郑州大学第三附属医院围产保健科 郑州 450052
Author(s):
YUAN Enwu1)WANG Yinfang1)XING Jinfang1)YAN Guangwei1)SUN Lihuan2)DAI Yanpeng1)
1)Department of Clinical Laboratory, the Third Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Department of Perinatal Health, the Third Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
稽留流产 绒毛组织 自噬 HIF-1α BNIP3 LC3
Keywords:
missed abortion villous tissue autophagy HIF-1α BNIP3 LC3
分类号:
R714.2
DOI:
10.13705/j.issn.1671-6825.2017.03.023
摘要:
目的:检测妊娠早期稽留流产患者绒毛组织中HIF-1α、BNIP3、LC3的表达,探讨滋养层细胞自噬异常与稽留流产的关系。方法:收集正常早孕妇女及稽留流产妇女绒毛组织各30例,采用实时荧光定量PCR法检测HIF-1α、BNIP3 mRNA的表达; 采用免疫组化SP法及蛋白印迹法检测HIF-1α、BNIP3及LC3蛋白的表达。结果:两组绒毛组织中均可见HIF-1α、BNIP3及LC3蛋白的表达,HIF-1α主要定位于滋养细胞胞核及胞质,BNIP3及LC3主要定位于滋养层细胞胞质中。与正常早孕组相比,稽留流产组HIF-1α与BNIP3 mRNA和蛋白表达水平以及LC3Ⅱ/Ⅰ均明显下降(P<0.05)。结论:绒毛组织滋养层细胞中HIF-1α/BNIP3信号通路调节的低氧诱导自噬水平下降可能是稽留流产发生的重要机制。
Abstract:
Aim: To investigate the expressions of HIF-1α,BNIP3,LC3 in villous tissue from with women early pregnancy missed abortion and the relationship between the abnormal autophagy in trophoblast cells and missed abortion.Methods: Villous tissue from healthy women with normal pregnancy(n=30)and women with missed abortion(n=30)were collected. RT-PCR was utilized to measure the expression levels of HIF-1α,BNIP3 mRNA; immunohistochemical SP method and Western blot were performed to determine the position and expression levels of HIF-1α,BNIP3 and LC3 Ⅱ/Ⅰprotein in villous tissue.Results: HIF-1α,BNIP3 and LC3 could be found in villous tissue from the two groups by immunochemical staining; HIF-1α was mainly localized in the nucleus and cytoplasm of trophoblast cells, BNIP3 and LC3 were mainly located in the cytoplasm of trophoblast cells. The mRNA expression of BNIP3 and protein expression levels of HIF-1α,BNIP3 and LC3Ⅱ/Ⅰ were significantly lower in villous tissue from missed abortion group than those from the normal control group(P<0.05).Conclusion: Autophagy mediated by HIF-1α/BNIP3 signaling pathway in the villous trophoblast cells may be the important mechanism of the occurrence of missed abortion.

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备注/Memo

备注/Memo:
*河南省科技厅创新团队项目 20140269
△男,1967年12月生,硕士,主任技师,教授,研究方向:临床生物化学与分子生物学,E-mail:yuanenwu@126.com
更新日期/Last Update: 2017-05-20