[1]赵先兰△,陶 雅,王永红,等.Notch信号通路配体Jagged1、Dll4在子痫前期胎盘组织中的表达[J].郑州大学学报(医学版),2017,(03):359-363.[doi:10.13705/j.issn.1671-6825.2017.03.029]
 ZHAO Xianlan,TAO Ya,WANG Yonghong,et al.Expressions of Notch ligand Jagged1 and Dll4 in preeclampsia placental tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(03):359-363.[doi:10.13705/j.issn.1671-6825.2017.03.029]
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Notch信号通路配体Jagged1、Dll4在子痫前期胎盘组织中的表达()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年03期
页码:
359-363
栏目:
应用研究
出版日期:
2017-05-15

文章信息/Info

Title:
Expressions of Notch ligand Jagged1 and Dll4 in preeclampsia placental tissue
作者:
赵先兰陶 雅王永红刘 传杜莹莹
郑州大学第一附属医院产科 郑州 450052
Author(s):
ZHAO XianlanTAO YaWANG YonghongLIU Chuan DU Yingying
Department of Gynecology,the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052
关键词:
子痫前期 Notch信号通路 Jagged1 Dll4 胎盘
Keywords:
preeclampsia Notch signaling pathway Jagged1 Dll4 placenta
分类号:
R714.24
DOI:
10.13705/j.issn.1671-6825.2017.03.029
摘要:
目的:探讨Notch信号通路配体Jagged1、Dll4在子痫前期胎盘组织中的表达及意义。方法:选取轻度子痫前期患者30例(轻度组)、重度子痫前期患者30例(重度组)、正常妊娠孕妇30例(对照组),采用免疫组化法检测其胎盘组织中Jagged1、Dll4的定位表达,采用荧光定量PCR技术和Western blot法分别检测胎盘组织中Jagged1、Dll4 mRNA及蛋白的表达。结果:Jagged1主要表达于血管内皮细胞和绒毛滋养层细胞,Dll4主要表达于血管内皮细胞。轻、重度组胎盘组织中Jagged1、Dll4 mRNA及蛋白的表达水平均低于对照组(P<0.05),且重度组低于轻度组(P<0.05); 对照组胎盘组织中Jagged1与Dll4在mRNA和蛋白水平上的表达均呈正相关(r=0.806和0.808,P<0.05),轻度组中两者表达的相关性减弱(r=0.675和0.560,P<0.05),而在重度组中未发现两者的相关性。结论:Jagged1、Dll4在子痫前期胎盘组织中表达失衡,与子痫前期的发病关系密切。
Abstract:
Aim: To explore the expressions of Notch ligand Jagged1 and Dll4 in preeclampsia placentas.Methods: Placental specimens from 30 normal pregnant women(control group),30 mild preeclampsia women(mild group)and 30 severe preeclampsia women(severe group)were collected to detect the location of Jagged1 and Dll4 protein by immunohisto chemistry; RT-qPCR and Western blot were used to determine the expressions of mRNA and protein of Jagged1 and Dll4, respectively.Results: Jagged1 was mainly expressed in vascular endothelial cells and trophoblast cells.Dll4 was expressed in vascular endothelial cells. The mRNA and protein expressions of Jagged1 and Dll4 in placentas from patients of mild group and severe group were significantly lower than those from control group(P<0.05), which was lowest in severe group(P<0.05). The mRNA and protein expressions of Jagged1 were positively correlated with those of Dll4 in control group(r=0.806,0.808,P<0.05), and weakened in mild group(r=0.675,0.560,P<0.05), but had no correlation in severe group.Conclusion: The imbalance of Jagged1 and Dll4 may be related to the occurrence of preeclampsia.

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备注/Memo

备注/Memo:
△女,1965年10月生,博士,主任医师,研究方向:高危妊娠,E-mail:ZXL12129@163.com
更新日期/Last Update: 2017-05-20