[1]彭 芳),崔渊博),宋文颖),等.远志提取物对阿尔茨海默病小鼠学习记忆能力的干预效果*[J].郑州大学学报(医学版),2017,(04):407-412.[doi:10.13705/j.issn.1671-6825.2017.04.008]
 PENG Fang),CUI Yuanbo),SONG Wenying),et al.Effect of radix polygalae on learning and memory ability of Alzheimer's disease mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(04):407-412.[doi:10.13705/j.issn.1671-6825.2017.04.008]
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远志提取物对阿尔茨海默病小鼠学习记忆能力的干预效果*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年04期
页码:
407-412
栏目:
论著
出版日期:
2017-07-15

文章信息/Info

Title:
Effect of radix polygalae on learning and memory ability of Alzheimer's disease mice
作者:
彭 芳1)崔渊博2)宋文颖1)张家薇1)刘俊保3)#关方霞4)#
1)郑州澍青医学高等专科学校临床医学系 郑州 450064;
2)郑州大学附属郑州中心医院转化医学中心 郑州 450007;
3)郑州大学人民医院中医科 郑州 450003;
4)郑州大学生命科学学院 郑州 450001
Author(s):
PENG Fang1)CUI Yuanbo2) SONG Wenying1)ZHANG Jiawei1)LIU Junbao3) GUAN Fangxia4)
1)Department of Clinical Medicine,Zhengzhou Shuqing Medical College,Zhengzhou 450064;
2)Translational Medicine Center,Zhengzhou Central Hospital Affiliated to Zhengzhou University,Zhengzhou 450007;
3)Department of Traditional Chinese Medicine,the People's Hospital of Zhengzhou University, Zhengzhou 450003;
4)School of Life Sciences, Zhengzhou University, Zhengzhou 450001
关键词:
远志提取物 阿尔茨海默病 学习记忆能力 小鼠
Keywords:
radix polygalae Alzheimer's disease learning and memory ability mouse
分类号:
R259
DOI:
10.13705/j.issn.1671-6825.2017.04.008
摘要:
目的:探讨远志提取物对阿尔茨海默病(AD)小鼠学习记忆能力的影响。方法:将28只6月龄雄性AD模型小鼠随机均分为4组,对照组和A、B、C组。A、B和C组小鼠连续3个月分别口服远志提取物50、100、200 mg/(kg·d),对照组小鼠口服等量溶剂。3个月后,采用Morris水迷宫实验检测小鼠学习记忆能力,采用ELISA法测定小鼠海马组织中可溶性与不溶性Aβ40、Aβ42含量及β分泌酶1(BACE1)、γ分泌酶活性,分别采用实时荧光定量PCR和Western blot法检测小鼠海马组织中脑啡肽酶(NEP)、胰岛素降解酶(IDE)、MMP-9 mRNA及蛋白的表达水平,采用Western blot法检测小鼠海马组织中脑源性神经营养因子(BDNF)、BDNF酪氨酸激酶受体B(TrkB)及磷酸化TrkB(p-TrkB)、环磷腺苷效应元件结合蛋白(CREB)及磷酸化CREB(p-CREB)的表达水平。结果:与对照组相比,C组小鼠穿越平台次数增加、逃避潜伏期缩短(P<0.05); A、B、C组小鼠海马组织中可溶性与不溶性Aβ40、Aβ42含量降低,NEP、IDE mRNA及蛋白表达水平升高,BDNF、p-TrkB及p-CREB蛋白表达水平升高(P均<0.05),且C组小鼠上述指标的变化更明显(P<0.05)。而3组小鼠海马组织中BACE1和γ分泌酶活性、MMP-9表达及TrkB、CREB蛋白表达水平无变化(P>0.05)。结论:远志提取物可有效改善AD小鼠的学习记忆能力,促进Aβ降解与调节BDNF/TrkB信号通路可能是其分子作用机制。
Abstract:
Aim: To investigate the effects of radix polygalae on learning and memory ability of Alzheimer's disease(AD)mice.Methods: A total of 28 6-month-old AD male mice were equally allocated into 4 groups(control group, group A, group B and group C). Mice in the above 4 groups took radix polygalae at the dose of 0(the same volume of solvent),50,100,200 mg/kg orally once a day for three months, respectively. Three months later, the learning and memory ability was detected by Morris water maze test; the contents of soluble and insoluble Aβ40 and Aβ42, the activity of BACE1 and γ-secretase in the hippocampus were examined by ELISA; the mRNA expressions of NEP,IDE and MMP-9 were measured by quantitative real time PCR; the protein expressions of NEP, IDE, MMP-9, BDNF, TrkB, p-TrkB, CREB and p-CREB in the hippocampus were tested by Western blot.Results: Compared with the control group, the number of platforms crossing increased, and the escape latency was shortened in group C; the soluble and insoluble Aβ40 and Aβ42 contents in the hippocampus of group A,B,C decreased, the mRNA and protein expressions of NEP and IDE were significantly upregulated, and the expression of BDNF, p-TrkB and p-CREB proteins in the hippocampus increased(P<0.05); the changes of the indexes mentioned above of group C improved significantly than group A and B(P<0.05). While the activity of BACE1 and γ-secretase, and MMP-9, TrkB, CREB expressions in the hippocampus showed no significant differences among the 4 groups(P>0.05).Conclusion: Radix polygalae could improve the learning and memory ability of AD mice, and degradation of Aβ and regulating BDNF/TrkB signaling pathway might be the possible molecular mechanism.

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备注/Memo

备注/Memo:
*河南省科技厅重大攻关项目 12210130200
#通信作者:刘俊保,男,1966年3月生,博士,主任医师,研究方向:中西医结合,E-mail:Liu-371@sohu.com; 关方霞,女,1969年2月生,博士,教授,研究方向:干细胞与再生医学、神经认知及调控,E-mail:guanfangxia@126.com
更新日期/Last Update: 2017-07-20