[1]程豪为△,杨荟玉,杨君霞,等.microRNA-509-3p对肝癌HepG2细胞增殖和侵袭能力的影响*[J].郑州大学学报(医学版),2017,(04):412-415.[doi:10.13705/j.issn.1671-6825.2017.04.009]
 CHENG Haowei,YANG Huiyu,YANG Junxia,et al.Effects of microRNA-509-3p on proliferation and invasiveness of liver carcinoma HepG2 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(04):412-415.[doi:10.13705/j.issn.1671-6825.2017.04.009]
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microRNA-509-3p对肝癌HepG2细胞增殖和侵袭能力的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年04期
页码:
412-415
栏目:
论著
出版日期:
2017-07-15

文章信息/Info

Title:
Effects of microRNA-509-3p on proliferation and invasiveness of liver carcinoma HepG2 cells
作者:
程豪为杨荟玉杨君霞孙爱民陈宏涛杨小昂巴秋菊
郑州大学医药科学研究院肝病研究室 郑州 450052
Author(s):
CHENG HaoweiYANG HuiyuYANG JunxiaSUN AiminCHEN HongtaoYANG Xiao'angBA Qiuju
Department of Liver Disease,Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450052
关键词:
肝癌 HepG2细胞 微小RNA-509-3p XIAP 细胞侵袭 增殖
Keywords:
liver carcinoma HepG2 cell microRNA-509-3p XIAP cell invasion proliferation
分类号:
R735.7
DOI:
10.13705/j.issn.1671-6825.2017.04.009
摘要:
目的:探讨微小RNA(miR)-509-3p对肝癌细胞增殖和侵袭能力的影响及其机制。方法:选取60例肝癌患者癌组织及癌旁组织,采用实时荧光定量PCR法检测miR-509-3p的表达。培养HepG2细胞,分别转染miR-509-3p类似物和miR-509-3p抑制剂或X连锁凋亡抑制蛋白(XIAP)siRNA,以不进行任何处理的细胞为空白对照,利用CCK-8试剂及Transwell小室分别检测miR-509-3p表达对HepG2细胞增殖和侵袭能力的影响。结果:miR-509-3p在肝癌组织中的表达低于癌旁组织(P<0.001)。miR-509-3p类似物组细胞增殖能力和细胞侵袭数均低于空白对照组(P均<0.05),miR-509-3p抑制剂组细胞侵袭数高于空白对照组(P<0.05),miR-509-3p过表达可抑制XIAP的表达(P<0.05)。结论:miR-509-3p表达对肝癌细胞的增殖和侵袭能力有抑制作用,其机制可能是miR-509-3p低表达促进XIAP上调从而促进肝癌细胞增殖、增加其侵袭能力。
Abstract:
Aim: To explore the role of microRNA(miR)-509-3p in proliferation and invasion of liver carcinoma cells.Methods: A total of 60 cases of liver carcinoma tissue and 60 cases of paracancerous tissue were selected, and the miR-509-3p expression was detected by real-time PCR.HepG2 cells were cultured and transfected with miR-509-3p mimic, miR-509-3p inhibitor or XIAP siRNA, and cells without any treatment were the control group.The cell proliferation activity was detected by CCK-8, and cell invasion was detected by Transwell cell migration assay.Results: The expression of miR-509-3p in liver carcinoma tissue was significantly lower than that in paracancerous tissue(P<0.001).Compared with the control group, the cell proliferation rate and the number of migration cells in miR-509-3p mimic group were significant lower(P<0.05), while those in miR-509-3p inhibitor group were higher(P<0.05),and miR-509-3p overexpression contributed to the inhibition of XIAP expression(P<0.05).Conclusion: miR-509-3p plays an important role in the development and progression of liver carcinoma, and its downregulation of miR-509-3p may be closely associated with the high expression of XIAP.

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备注/Memo

备注/Memo:
*河南省基础与前沿项目 142300410326
△男,1962年6月生,本科,高级实验师,研究方向:肝病,E-mail:aiminsunzz@163.com
更新日期/Last Update: 2017-07-20