[1]穆立华),张丽华)#,蒋友旭),等.左西孟旦对LPS诱导的乳鼠心肌细胞损伤的保护作用*[J].郑州大学学报(医学版),2017,(04):427-430.[doi:10.13705/j.issn.1671-6825.2017.04.013]
 MU Lihua),ZHANG Lihua),JIANG Youxu),et al.Protective effect of simdax on LPS-induced neonatal rat cardiomyocyte injury[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(04):427-430.[doi:10.13705/j.issn.1671-6825.2017.04.013]
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左西孟旦对LPS诱导的乳鼠心肌细胞损伤的保护作用*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年04期
页码:
427-430
栏目:
应用研究
出版日期:
2017-07-15

文章信息/Info

Title:
Protective effect of simdax on LPS-induced neonatal rat cardiomyocyte injury
作者:
穆立华1)张丽华1)#蒋友旭1)李小威1)朱英丽2)
1)郑州大学第二附属医院心血管内科 郑州 450014;
2)河南省人民医院临床药理室 郑州 450003
Author(s):
MU Lihua1)ZHANG Lihua1)JIANG Youxu1)LI Xiaowei1)ZHU Yingli2)
1)Department of Vasculocardiology, the Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014;
2)Department of Clinical Pharmacology, Henan Provincial People's Hospital, Zhengzhou 450003
关键词:
左西孟旦 LPS 心肌细胞 损伤 乳鼠
Keywords:
simdax LPS cardiomyocyte injury neonatal rat
分类号:
R542.2
DOI:
10.13705/j.issn.1671-6825.2017.04.013
摘要:
目的:探讨左西孟旦对LPS诱导的乳鼠心肌细胞损伤的保护作用。方法:将原代培养的SD乳鼠的心肌细胞分为3组:对照组、模型组和左西孟旦干预组。模型组和左西孟旦干预组用10 mg/L的LPS处理6 h建模,其中左西孟旦干预组于造模前用终浓度为0.30 μmol/L的左西孟旦预处理24 h。全自动生化仪检测3组细胞培养液LDH、CK含量; ELISA检测培养液TNF-α、IL-6含量; 流式细胞仪检测细胞凋亡; Western blot检测凋亡相关蛋白Cleaved-Caspase-3及抗凋亡蛋白Bcl-2的表达。结果:与对照组比较,模型组细胞培养液中LDH、CK、TNF-α和IL-6含量增加,细胞凋亡率升高,凋亡蛋白Cleaved-Caspase-3表达增高,抗凋亡蛋白Bcl-2表达降低(P<0.05); 与模型组比较,左西孟旦干预组细胞培养液中LDH、CK、TNF-α、IL-6含量减少,细胞凋亡率降低,Cleaved-Caspase-3表达量降低,Bcl-2表达增高(P<0.05)。结论:左西孟旦对LPS诱导的乳鼠心肌细胞损伤有保护作用,其机制可能与减少炎症因子释放,抑制Caspase-3蛋白的表达,促进Bcl-2蛋白的表达有关。
Abstract:
Aim: To investigate the protective effect of simdax on lipopolysaccharide(LPS)-induced neonatal rat cardiomyocyte injury.Methods: The neonatal rat cardiomyocytes were allocated into 3 groups: control group, LPS group and LPS+simdax group. The cells in LPS+simdax group were pretreated with simdax(0.30 μmol/L)for 24 h. The cells in LPS group and LPS+simdax group were incubated with LPS(10 mg/L)for 6 h. The contents of LDH and CK in the culture medium were tested by automatic biochemistry analyzer,and those of TNF-α and IL-6 were evaluated by ELISA; the apoptosis was determined by flow cytometry; the expressions of Cleaved-Caspase-3 and Bcl-2 were measured by Western blot.Results: Compared with control group,the contents of LDH, CK, TNF-α and IL-6 as well as the apoptosis rate in LPS group were elevated significantly; the expression of Cleaved-Caspase-3 was increased apparently while that of Bcl-2 was reduced significantly(P<0.05). However, compared with LPS group, the contents of LDK, CK, TNF-α and IL-6 and the apoptosis rate in LPS+simdax group were significantly decreased, the expression of Cleaved-Caspase-3 was reduced, and that of Bcl-2 was increased(P<0.05).Conclusion: Simdax may inhibit the LPS-induced neonatal rat cardiomyocyte injury via decreasing the production of proinflammatory cytokines, down-regulating the expression of Caspase-3 and up-regulating the expression of Bcl-2.

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备注/Memo

备注/Memo:
*河南省医学科技攻关计划普通项目 201602103
#通信作者,女,1964年3月生,硕士,教授,主任医师,研究方向:冠心病的基础与临床,E-mail:zlhxp@126.com
更新日期/Last Update: 2017-07-20