[1]李保平),田 捧),李 莉),等.DGCR8在先天性心脏病患者血液及心肌组织中的表达*[J].郑州大学学报(医学版),2017,(04):431-434.[doi:10.13705/j.issn.1671-6825.2017.04.014]
 LI Baoping),TIAN Peng),LI Li),et al.Expression of DGCR8 in blood samples and myocardial tissue from children with congenital heart disease[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(04):431-434.[doi:10.13705/j.issn.1671-6825.2017.04.014]
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DGCR8在先天性心脏病患者血液及心肌组织中的表达*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年04期
页码:
431-434
栏目:
应用研究
出版日期:
2017-07-15

文章信息/Info

Title:
Expression of DGCR8 in blood samples and myocardial tissue from children with congenital heart disease
作者:
李保平1)田 捧1)李 莉1)赵冠男2)武海英3)岳军明2)李 群1)范太兵3)任琛琛1)郭玉琪14)#
1)郑州大学第三附属医院妇产科 郑州 450052;
2)田纳西大学健康科学中心病理医学实验室 孟菲斯,田纳西州,美国 38163;
3)河南省人民医院儿童心脏中心 郑州 450003;
4)河南省妇科肿瘤纳米医学国际联合实验室 郑州 450003
Author(s):
LI Baoping1)TIAN Peng1)LI Li1)ZHAO Guannan2)WU Haiying3)YUE Junming2)LI Qun1)FAN Taibing3)REN Chenchen1)GUO Yuqi14)
1)Department of Gynecology and Obstetrics, the Third Affiliated Hospital, Zhengzhou University, Zhengzhou 450052;
2)Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center Memphis,TN,USA 38163;
3)Pediatric Heart Center, Henan Provincial People's Hospital, Zhengzhou 450003;
4)International Joint Laboratory for Gynecological Oncology Nanomedicine of Henan Province, Zhengzhou 450003
关键词:
迪乔治综合征危象区基因 8 先天性心脏病 室间隔缺损 法洛四联症
Keywords:
DGCR8 congenital heart disease ventricular septal defect tetralogy of Fallot
分类号:
R725.4
DOI:
10.13705/j.issn.1671-6825.2017.04.014
摘要:
目的:探讨迪乔治综合征危象区基因8(DGCR8)与先天性心脏病(CHD)发生的关系及临床意义。方法:收集CHD患儿及健康儿童血液样本各40份,采用qRT-PCR方法检测DGCR8 mRNA的表达; 收集室间隔缺损(VSD)患儿心肌组织25份,法洛四联症(TOF)患儿心肌组织16份,采用qRT-PCR和Western blot方法检测DGCR8 mRNA和蛋白的表达,分析间隔缺损患儿血液中DGCR8的表达水平与心脏间隔缺损大小的相关性。结果:与健康儿童相比,CHD患儿血液中DGCR8 mRNA的表达量降低(P=0.037); 与VSD患儿相比,TOF患儿心肌组织中DGCR8 mRNA和蛋白的表达量降低(P<0.05); DGCR8与心脏间隔缺损无明显相关性(rS=-0.022,P=0.917)。结论:DGCR8基因的缺失与CHD的发生有关,影响心脏的正常发育。
Abstract:
Aim: To explore the expression and clinical significance of DGCR8 in children with congenital heart disease(CHD).Methods: The blood samples of 40 children with CHD and 40 healthy children were collected,respectively.qRT-PCR method was used to detect the expression of DGCR8 mRNA. The expression of DGCR8 in myocardial tissue from 25 children with ventricular septal defect(VSD)and 16 children with tetralogy of Fallot(TOF)was detected by qRT-PCR and Western blot,and the correlation between DGCR8 and septal defect was analyzed.Results: Compared with healthy children, the expression of DGCR8 in blood from children with CHD was decreased(P=0.037).The expressions of DGCR8 mRNA and protein in myocardial tissue from those with TOF were lower compared with VSD(P<0.05).There was no significant correlation between DGCR8 and the septal defect(rS=-0.022, P=0.917).Conclusion: The deletion of DGCR8 gene is associated with the development of CHD and affects the normal development of the heart.

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备注/Memo

备注/Memo:
*国家自然科学基金面上项目 81572574; 河南省高校科技创新团队项目 16IRTSTHN018; 郑州大学创新项目 DYCX053
#通信作者,女,1964年1月生,博士,教授,研究方向:出生缺陷、卵巢癌,E-mail:yuqi-guo@163.com
更新日期/Last Update: 2017-07-20