[1]杜 娟,左非非,魏双艳,等.山楂降脂软胶囊中4种黄酮含量的测定*[J].郑州大学学报(医学版),2017,(04):456-459.[doi:10.13705/j.issn.1671-6825.2017.04.020]
 DU Juan,ZUO Feifei,WEI Shuangyan,et al.Simultaneous quantification of four flavonoids in hawthorn lipid-lowering soft capsules[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(04):456-459.[doi:10.13705/j.issn.1671-6825.2017.04.020]
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山楂降脂软胶囊中4种黄酮含量的测定*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年04期
页码:
456-459
栏目:
应用研究
出版日期:
2017-07-15

文章信息/Info

Title:
Simultaneous quantification of four flavonoids in hawthorn lipid-lowering soft capsules
作者:
杜 娟左非非魏双艳徐晓阳张玮杰阴兆静徐 霞#
郑州大学药学院 郑州 450001
Author(s):
DU Juan ZUO Feifei WEI Shuangyan XU Xiaoyang ZHANG Weijie YIN Zhaojing XU Xia
College of Pharmaceutical Science, Zhengzhou University, Zhengzhou 450001
关键词:
山楂降脂软胶囊 葛根素 木犀草素 槲皮素 儿茶素 液质联用
Keywords:
hawthorn lipid-lowering soft capsule puerarin mignonette quercetin catechin UPLC-MS/MS
分类号:
R917
DOI:
10.13705/j.issn.1671-6825.2017.04.020
摘要:
目的:建立同时测定山楂降脂软胶囊中4种主要黄酮葛根素、木犀草素、槲皮素及儿茶素的超高效液相-串联质谱法。方法:在超声条件下用甲醇提取山楂降脂软胶囊供试品。采用Waters ACQUITY UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7 μm)色谱柱分离,以体积分数0.1%甲酸乙腈和体积分数0.1%甲酸水溶液为流动相梯度洗脱,在负离子模式下采用多反应监测。结果:葛根素、木犀草素、槲皮素、儿茶素分别在0.03~8.10、0.04~8.27、0.02~4.45及0.01~3.11 mg/L的范围内线性良好,R2均>0.992,4种黄酮的平均加样回收率在93.6%~107.5%,相对标准偏差在1.62%~2.21%。结论:建立了适用于快速测定山楂降脂软胶囊中4种黄酮的液质联用定量方法,该方法灵敏、准确、稳定,可用于山楂降脂软胶囊的质量控制。
Abstract:
Aim: To develop a quantitative method for simultaneous determination of puerarin, mignonette, quercetin and catechin in hawthorn lipid-lowering soft capsules using ultra performance liquid chromatography-tandem triple quadrupole mass spectrometry.Methods: Hawthorn lipid-lowering soft capsules were extracted with methanol under the condition of ultrasound. The separation was carried out on a Waters ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7 μm)column. And the mobile phase was consisted of acetonitrile-0.1%(V/V)formic acid and H2O-0.1%(V/V)formic acid in a gradient elution mode. Then the sample solution was analyzed in electrospray ionization-ion mode with multiple reaction monitoring.Results: The results suggested that puerarin, mignonette, quercetin and catechin exhibited a good linearity in the range of 0.03~8.10,0.04~8.27,0.02~4.45 and 0.01~3.11 mg/L, respectively. And the correlation coefficient was higher than 0.992. The average recovery rate of the 4 flavonoids was in the range of 93.6%~107.5%, while the range of relative standard deviation was 1.62%~2.21%.Conclusion: The established method is sensitive, accurate and stable, and meet the requirement for determination of the 4 major flavonoids in hawthorn lipid-loweringsoft capsules, thus could be used for quality control of hawthorn lipid-lowering soft capsules.

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备注/Memo

备注/Memo:
*河南省基础与前沿技术基金资助项目 142300410085; 郑州市国际科技合作与交流项目 153PGJHZ204
#通信作者,女,1965年4月生,博士,教授,研究方向:药物分析,E-mail:xuxia@zzu.edu.cn
更新日期/Last Update: 2017-07-20