[1]石 洁,朱岩昆,郑丹薇,等.河南省维生素D受体基因rs2222570和rs731236位点多态性与结核病的易感性*[J].郑州大学学报(医学版),2017,(05):553-558.[doi:10.13705/j.issn.1671-6825.2017.05.009]
 SHI Jie,ZHU Yankun,ZHENG Danwei,et al.Association of polymorphisms of rs2222570 and rs731236 in vitamin D receptor gene with tuberculosis susceptibility in Henan Province[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(05):553-558.[doi:10.13705/j.issn.1671-6825.2017.05.009]
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河南省维生素D受体基因rs2222570和rs731236位点多态性与结核病的易感性*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年05期
页码:
553-558
栏目:
论著
出版日期:
2017-09-15

文章信息/Info

Title:
Association of polymorphisms of rs2222570 and rs731236 in vitamin D receptor gene with tuberculosis susceptibility in Henan Province
作者:
石 洁朱岩昆郑丹薇马晓光王少华李 辉邢 进#吴彩霞
河南省疾病预防控制中心 郑州 450016
Author(s):
SHI Jie ZHU Yankun ZHENG Danwei MA Xiaoguang WANG Shaohua LI Hui XING Jin WU Caixia
Henan Province Center for Disease Control and Prevention,Zhengzhou 450016
关键词:
结核病 维生素D受体基因 单核苷酸多态性 多因子降维法
Keywords:
tuberculosis vitamin D receptor gene single nucleotide polymorphisms multifactor dimensionality reduction
分类号:
R183.3; R521
DOI:
10.13705/j.issn.1671-6825.2017.05.009
摘要:
目的:探讨河南省人群维生素D受体(VDR)基因多态性和结核病易感性之间关系。方法:采用病例对照研究方法,病例组纳入260个结核病患者,并以年龄和性别相匹配纳入260名健康人作为对照组。采用PCR-SSCP法对VDR基因位点多态性进行分析。应用SPSS 19.0对数据进行统计分析,SHEsis在线分析2个位点单倍体型和结核病发病的关联,MDR软件3.0.2分析基因和环境交互作用对结核病发病的影响。结果:VDR基因rs2222570位点多态性与结核病发病风险相关,野生型C/C降低了结核病发病50%的风险(校正 OR=0.501,95%CI:0.343~0.709),并且该位点等位基因C在对照组中高于病例组(校正OR=0.632, 95%CI: 0.493~0.868); 而VDR基因的rs731236位点突变基因型C/C增加结核病发病风险(校正OR=2.001, 95%CI: 1.838~2.191)。VDR基因单倍体型CT、TT和TC与结核病发病相关[CT:OR(95%CI)=0.673(0.521~0.847), P<0.001; TT: OR(95%CI)=1.332(1.043~1.711),P=0.032; TC: OR(95%CI)=6.561(2.366~18.143), P<0.001]。MDR结果表明VDR 基因rs2222570和rs731236位点多态性、吸烟、结核病患者密切接触史4个因素交互作用增加了结核病发病风险[OR(95%CI)=7.619(2.201~26.354),P<0.001]。结论:VDR基因多态性与结核病发病风险相关,且VDR 基因rs2222570和rs731236位点多态性、吸烟、结核病患者密切接触史存在交互作用。
Abstract:
Aim: To evaluate whether the vitamin D receptor(VDR)gene polymorphism or its haplotypes might be associated with tuberculosis susceptibility in Henan Han population.Methods: A total of 260 patients with tuberculosis were recruited, and 260 healthy control matched by sex and age were chosen. Genetic polymorphisms of rs2222570 and rs731236 in VDR gene were analyzed using PCR-SSCP. The data were analyzed by SPSS 19.0. The comparison of haplotypes were carried out by SHEsis online.The MDR 3.0.2 software was applied to analyze gene-environment interaction on the risk of tuberculosis susceptibility.Results: The variant genotypes of rs2222570 in VDR gene were significantly associ ated with the risk of tuberculosis, and the risk of individuals carrying wildtype genotype C/C decreased by 50%(adjusted OR=0.501,95%CI:0.343-0.709). The frequency of allele C in rs2222570 locus was significantly higher in control group compared with the tuberculosis group(adjusted OR=0.632, 95%CI:0.493-0.868). Whereas the mutant genotype C/C of VDR gene rs731236 polymorphism increased the risk of tuberculosis(adjusted OR=2.001, 95%CI:1.838-2.191).Haplotype analysis showed significant differences in the frequency of haplotype CT,TT,and TC between the two groups[CT: OR(95%CI)=0.673(0.521-0.847), P<0.001; TT:OR(95%CI)=1.332(1.043-1.711), P=0.032; TC:OR(95%CI)=6.561(2.366-18.143), P<0.001]. The interaction of the four factor model, rs2222570,rs731236 of VDR genotypes polymorphisms,smoking and the history of exposure to tuberculosis, might increase the risk of tuberculosis [OR(95%CI)=7.619(2.201-26.354), P<0.001].Conclusion: The polymorphism of rs2222570 and rs731236 in VDR gene are associated with the risk of tuberculosis, and there may be significant interaction between the rs2222570,rs731236 of VDR genotypes polymorphisms,smoking and history of exposure to tuberculosis.

参考文献/References:

[1] WHO publishes Global tuberculosis report 2013[J]. Euro Surveill, 2013,18(43).pii: 20615
[2] 王丹妹,何佟,吉丽敏,等.中国海南黎族人群LMP2/LMP7基因多态性与肺结核病的关联性[J].吉林大学学报(医学版),2013,39(4):786
[3] CANTORNA MT,SNYDER L,LIN YD,et al.Vitamin D and 1,25(OH)2D regulation of T cells[J].Nutrients,2015,7(4):3011
[4] FABRI M,STENGER S,SHIN DM,et al.Vitamin D is required for IFN-gamma-mediated antimicrobial activity of human macrophages[J].Sci Transl Med,2011,3(14):104ra102
[5] VELDMAN CM,CANTORNA MT,DELUCA HF.Expression of 1,25-dihydroxyvitamin D3 receptor in the immune system[J].Arch Biochem Biophys,2000,374(2):334
[6] HAUSSLER MR,WHITFIELD GK,KANEKO I,et al.Molecular mechanisms of vitamin D action[J].Calcif Tissue Int,2013,92(2):77
[7] 石洁,朱岩昆,郑丹薇,等.维生素D受体基因rs2222570位点多态性与结核发病相关性的meta分析[J].郑州大学学报(医学版),2016,51(6):774
[8] SELVARAJ P,CHANDRA G,JAWAHAR MS,et al.Regulatory role of vitamin D receptor gene variants of Bsm Ⅰ, Apa Ⅰ, Taq Ⅰ, and Fok Ⅰ polymorphisms on macrophage phagocytosis and lymphoproliferative response to mycobacterium tuberculosis antigen in pulmonary tuberculosis[J].J Clin Immunol,2004,24(5):523
[9] GIBNEY KB,MACGREGOR L,LEDER K,et al.Vitamin D deficiency is associated with tuberculosis and latent tuberculosis infection in immigrants from sub-Saharan Africa[J].Clin Infect Dis,2008,46(3):443
[10]NYLÉN H,HABTEWOLD A,MAKONNEN E,et al.Prevalence and risk factors for efavirenz-based antiretroviral treatment-associated severe vitamin D deficiency:a prospective cohort study[J].Medicine(Baltimore),2016,95(34):e4631
[11]FERNÁNDEZ-MESTRE M,VILLASMIL Á,TAKIFF H,et al.NRAMP1 and VDR gene polymorphisms in susceptibility to tuberculosis in Venezuelan population[J].Dis Markers,2015,2015:860628
[12]LIU W,CAO WC,ZHANG CY,et al.VDR and NRAMP1 gene polymorphisms in susceptibility to pulmonary tuberculosis among the Chinese Han population: a case-control study[J].Int J Tuberc Lung Dis,2004,8(4):428
[13]BORNMAN L,CAMPBELL SJ,FIELDING K,et al.Vitamin D receptor polymorphisms and susceptibility to tuberculosis in West Africa: a case-control and family study[J].J Infect Dis,2004,190(9):1631
[14]BELLAMY R,RUWENDE C,CORRAH T,et al.Tuberculosis and chronic hepatitis B virus infection in Africans and variation in the vitamin D receptor gene[J].J Infect Dis,1999,179(3):721
[15]SELVARAJ P,NARAYANAN PR, REETHA AM.Association of vitamin D receptor genotypes with the susceptibility to pulmonary tuberculosis in female patients & resistance in female contacts[J]. Indian J Med Res, 2000,111:172
[16]ROTH DE,SOTO G,ARENAS F,et al.Association between vitamin D receptor gene polymorphisms and response to treatment of pulmonary tuberculosis[J].J Infect Dis,2004,190(5):920
[17]ARJI N,BUSSON M,IRAQI G,et al.Genetic diversity of TLR2, TLR4, and VDR loci and pulmonary tuberculosis in Moroccan patients[J].J Infect Dev Ctries,2014,8(4):430
[18]BABB C,VAN DER MERWE L,BEYERS N,et al.Vitamin D receptor gene polymorphisms and sputum conversion time in pulmonary tuberculosis patients[J].Tuberculosis(Edinb),2007,87(4):295
[19]杨群,江晓静.结核蛋白芯片对肺结核及肺外结核的诊断价值[J].西安交通大学学报(医学版),2012,33(1):129
[20]苏倩,向颖,胡代玉,等.VDR基因多态性、环境因素与重庆地区肺结核病的联系及交互作用研究[J].局解手术学杂志,2012,21(4):361

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备注/Memo

备注/Memo:
*“十二·五”科技重大专项课题 2014ZX10003002; 河南省卫生计生委普通科技攻关项目 201702277
#通信作者,女,1966年6月生,硕士,主任医师,研究方向:结核病防控,E-mail:hncdcxj@163.com
更新日期/Last Update: 2017-09-20