[1]樊长晖,许长宝,郝 斌,等.缺氧下膀胱癌细胞黏附能力和侵袭能力的变化*[J].郑州大学学报(医学版),2017,(05):578-580.[doi:10.13705/j.issn.1671-6825.2017.05.015]
 FAN Changhui,XU Changbao,HAO Bin,et al.Effects of hypoxia on adhesiveness and invasiveness of bladder carcinoma cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(05):578-580.[doi:10.13705/j.issn.1671-6825.2017.05.015]
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缺氧下膀胱癌细胞黏附能力和侵袭能力的变化*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年05期
页码:
578-580
栏目:
论著
出版日期:
2017-09-15

文章信息/Info

Title:
Effects of hypoxia on adhesiveness and invasiveness of bladder carcinoma cells
作者:
樊长晖许长宝 郝 斌吴国清赵兴华#
郑州大学第二附属医院泌尿外科 郑州 450014
Author(s):
FAN Changhui XU Changbao HAO Bin WU Guoqing ZHAO Xinghua
Department of Urology, the Second Affiliated Hospital,Zhengzhou University, Zhengzhou 450014
关键词:
膀胱癌 缺氧 黏附 迁移
Keywords:
bladder carcinoma hypoxia adhesiveness migration
分类号:
R737.14
DOI:
10.13705/j.issn.1671-6825.2017.05.015
摘要:
目的:探讨缺氧条件下不同分化膀胱癌细胞5637和T24生物学特性变化。方法:将不同分化程度的膀胱癌细胞5637和T24分别置于正常氧浓度条件下(37 ℃, 体积分数5% CO2和体积分数21% O2)和缺氧(37 ℃、体积分数10%O2、体积分数5% CO2和体积分数85%N2)环境中培养,以MTT法和Boyden侵袭小室法检测两种膀胱癌细胞在不同环境下黏附能力和侵袭能力的差异。结果:随着培养时间的延长,缺氧组和对照组5637和T24细胞的黏附率逐渐增加,差异有统计学意义(P<0.001); 5637和T24细胞黏附率在缺氧24 h与对照组相比差异无统计学意义(P>0.05),但缺氧48 h和72 h两种细胞的黏附能力均高于对照组(P<0.05); 缺氧组5637和T24细胞的穿膜细胞数均高于对照组(P<0.001)。结论:缺氧条件下膀胱癌细胞的黏附和迁移能力增强。
Abstract:
Aim: To study the effects of hypoxia on adhesion and migration of bladder carcinoma cell line 5637 and T24 in vitro.Methods: The bladder carcinoma cells 5637 and T24 were cultured in hypoxic condition(10%O2,5% CO2,85%N2 mixed gas), while those put into 5% CO2,37 ℃ incubator were control group. The influence of hypoxia on the adhesiveness and invasiveness of the 2 kinds of cells were detected by MTT and Boyden method, respectively.Results: The result showed that the adhesiveness ability of 5637 cells and T24 cells was enhanced with culture time in hypoxic condition and the normal oxygen condition(P<0.001). Compared with those cultured in normal oxygen condition, the adhensiveness of the 2 cells cultured in hypoxic condition did not change significantly after 24 h culture(P>0.05), but increased after 48 or 72 h culture(P<0.05).The migration ability of the 2 kinds of cells was enhanced after cultured in hypoxic condition than that of control group(P<0.001).Conclusion: The adhesiveness and migration ability of bladder carcinoma cells are enhanced in hypoxic condition.

参考文献/References:

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[5] CHOUAIB S,NOMAN MZ,KOSMATOPOULOS K,et al.Hypoxic stress: obstacles and opportunities for innovative immunotherapy of cancer[J].Oncogene,2017,36(4):439
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备注/Memo

备注/Memo:
*河南省卫生科技创新性人才工程中青年科技创新人才基金资助项目 4118
#通信作者,男,1965年10月生,本科,教授,研究方向:泌尿系肿瘤,E-mail:495975452@qq.com
更新日期/Last Update: 2017-09-20