[1]马 星),陈新峰),张超奇),等.非小细胞肺癌组织中MAGE-A3和MAGE-C2 mRNA的表达*[J].郑州大学学报(医学版),2017,(05):580-585.[doi:10.13705/j.issn.1671-6825.2017.05.016]
 MA Xing),CHEN Xinfeng),ZHANG Chaoqi),et al.Expressions of MAGE-A3 and MAGE-C2 mRNA in non-small cell lung carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(05):580-585.[doi:10.13705/j.issn.1671-6825.2017.05.016]
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非小细胞肺癌组织中MAGE-A3和MAGE-C2 mRNA的表达*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年05期
页码:
580-585
栏目:
应用研究
出版日期:
2017-09-15

文章信息/Info

Title:
Expressions of MAGE-A3 and MAGE-C2 mRNA in non-small cell lung carcinoma tissue
作者:
马 星1)陈新峰2)张超奇2)乔亚敏2)张 毅12)王丽萍1)#
1)郑州大学第一附属医院肿瘤科 郑州 450052 2)郑州大学第一附属医院生物细胞治疗中心 郑州 450052
Author(s):
MA Xing1) CHEN Xinfeng2) ZHANG Chaoqi2) QIAO Yamin2) ZHANG Yi12) WANG Liping1)
1)Department of Oncology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Biotherapy Center, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
非小细胞肺癌 MAGE-A3 MAGE-C2
Keywords:
non-small cell lung cancer MAGE-A3 MAGE-C2
分类号:
R734.2
DOI:
10.13705/j.issn.1671-6825.2017.05.016
摘要:
目的:检测肿瘤相关抗原恶性黑色素瘤相关抗原(MAGE)-A3、MAGE-C2在非小细胞肺癌(NSCLC)中的表达情况,并探讨其临床意义。方法:收集206例NSCLC手术患者肿瘤组织,应用real-time PCR技术检测组织中 MAGE-A3、MAGE-C2 mRNA的表达,并分析其与NSCLC患者临床病理特征(性别、年龄、组织学分级、病理类型、淋巴结转移和临床分期)之间的关系。用基因沉默的方法将肺癌A549细胞MAGE-A3下调,观察处理前后MAGE-A3 mRNA表达水平的改变,并分析其对细胞生物学功能(凋亡率、成球率、穿膜细胞数)的影响。结果:MAGE-A3、MAGE-C2 mRNA在NSCLC组织中的表达率分别是73.3%(151/206)和52.9%(109/206); MAGE-A3 mRNA的表达与疾病分期和淋巴结转移有关(P<0.05); 不同病理特征的NSCLC组织中MAGE-C2 mRNA的表达比较,差异无统计学意义(P>0.05); MAGE-A3、MAGE-C2 mRNA共表达与年龄和临床分期有关(P<0.05)。MAGE-A3表达降低后肺癌A549细胞的成球能力和侵袭转移能力降低(P<0.001)。结论:MAGE-A3、MAGE-C2在NSCLC组织中有较高的表达率, 两者有望成为肺癌免疫治疗的潜在靶点。
Abstract:
Aim: To analyze the expressions of MAGE-A3 and MAGE-C2 mRNA in non-small cell lung cancer(NSCLC)tissue and the relationship between their expressions and clinical pathological characteristics.Methods: Tumor tissue was collected from 206 patients diagnozed as NSCLC. Real-time PCR was performed to detect the expressions of MAGE-A3 and MAGE-C2 mRNA. The correlation of clinicopathological characteristics(gender, age, histologic grading, pathological type, lymph node metastasis, clinical stage)with the expressions of MAGE-A3 and MAGE-C2 mRNA in NSCLC tissue was evaluated. Then siRNA was used to down-regulate the expression of MAGE-A3 in A549 cells and the effects of MAGE-A3 knockdown on biology behavior of A549 cells were observed.Results: The mRNA expression rates of MAGE-A3 and MAGE-C2 in NSCLC tissue were 73.3%(151/206)and 52.9%(109/206), respectively. The mRNA expression of MAGE-A3 was associated with clinical stage and lymph node metastasis(P<0.05). However, there was no relation between MAGE-C2 mRNA expression and clinical characteristics(P>0.05). The mRNA coexpression of MAGE-A3 and MAGE-C2 was associated with age and clinical stage(P<0.05).MAGE-A3 knockdown led to decrease of sphere formation, invasion, and metastasis abilities of cells(P<0.001).Conclusion: MAGE-A3 and MAGE-C2 are expressed in NSCLC at high level; MAGE-A3 and MAGE-C2 might be potential target antigens for immunotherapy of NSCLC.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目 81171986,81271815; 科技部国家重点研发计划 2016YFC1303500
#通信作者,女,1963年5月生,博士,教授,研究方向:肿瘤的化疗和免疫治疗,E-mail:wlp@zzu.edu.cn
更新日期/Last Update: 2017-09-20