[1]史会影),刘振华),占卫红),等.复合抗原ESAT-6/CFP-10/Rv3615c ELISPOT辅助诊断活动性肺结核的应用价值*[J].郑州大学学报(医学版),2017,(05):629-633.[doi:10.13705/j.issn.1671-6825.2017.05.028]
 SHI Huiying),LIU Zhenhua),ZHAN Weihong),et al.Diagnositc value of ELISPOT by multiple antigens in detection of active pulmonary tuberculosis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(05):629-633.[doi:10.13705/j.issn.1671-6825.2017.05.028]
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复合抗原ESAT-6/CFP-10/Rv3615c ELISPOT辅助诊断活动性肺结核的应用价值*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年05期
页码:
629-633
栏目:
应用研究
出版日期:
2017-09-15

文章信息/Info

Title:
Diagnositc value of ELISPOT by multiple antigens in detection of active pulmonary tuberculosis
作者:
史会影1)刘振华2)占卫红3)雷 航3)王 琳4)梁瑞霞5)任伟宏6)汤兵祥5)#
1)郑州大学第一附属医院呼吸内科 郑州 450052 2)复旦大学附属闵行中心医院检验科 上海201100 3)上海交通大学基础医学院病原生物学教研室 上海 200025 4)复旦大学附属公共卫生临床中心 上海 200540 5)河南省胸科医院呼吸内科 郑州 450008 6)河南中医药大学第一附属医院检验科 郑州 450008
Author(s):
SHI Huiying1)LIU Zhenhua2) ZHAN Weihong3) LEI Hang3) WANG Lin4) LIANG Ruixia5) REN Weihong6) TANG Bingxiang5)
1)Department of Respiratory Medicine, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Department of Clinical Laboratory, Minhang Central Hospital, Fudan University, Shanghai 201100 3)Department of Pathogenic Biology, College of Basic Medical Sciences, Shanghai Jiaotong University, Shanghai 200025 4)Clinical Center of Public Health, Fudan University, Shanghai 200540 5)Department of Respiratory Medicine, Henan Chest Hospital, Zhengzhou 450008 6)Department of Clinical Laboratory, the First Affiliated Hospital, Henan University of Traditional Chinese Medicine, Zhengzhou 450008
关键词:
肺结核 ESAT-6 CFP-10 Rv3615c
Keywords:
pulmonary tuberculosis ESAT-6 CFP-10 Rv3615c
分类号:
R521
DOI:
10.13705/j.issn.1671-6825.2017.05.028
摘要:
目的:评估结核分枝杆菌复合抗原ESAT-6/CFP-10/Rv3615c在活动性肺结核诊断中的应用价值。方法:选取肺结核病患者78例,非结核性肺部疾病患者27例和健康体检者39例,分别用复合抗原ESAT-6/CFP-10/Rv3615c、ESAT-6肽段库和CFP-10肽段库作为抗原刺激单个核淋巴细胞(PBMCs),采用酶联免疫斑点试验检测斑点形成细胞数,判断肺结核感染情况。结果:复合抗原ESAT-6/CFP-10/Rv3615c、ESAT-6肽段库和CFP-10肽段库检测结核分枝杆菌感染的敏感性依次为87.2%(68/78)、80.8%(63/78)、76.9%(60/78),特异性依次为81.8%(54/66)、84.8%(56/66)、87.9%(58/66)。结论:复合抗原ESAT-6 /CFP-10 /Rv3615c有较高的敏感性和特异性,对活动性肺结核的辅助诊断有一定的临床应用价值。
Abstract:
Aim: To evaluate the value of mycobacterium tuberculosis composite antigen ESAT-6/CFP-10/Rv3615c in the diagnosis of active pulmonary tuberculosis.Methods: Seventy-eight patients with pulmonary tuberculosis, 27 patients with non-tuberculous lung disease and 39 healthy control were selected. The peripheral blood mononuclear cells(PBMCs)were stimulated with the composite antigen ESAT-6/CFP-10/Rv3615c, ESAT-6 peptide or CFP-10 peptide. SFCs was detected by ELISPOT to determine the situation of tuberculosis infection.Results: The sensitivities of mycobacterium tuberculosis infection detected by the composite antigen ESAT-6/CFP-10/Rv3615c, ESAT-6 peptide and CFP-10 peptide were 87.2%(68/78), 80.8%(63/78), 76.9%(60/78), respectively, and the specificities were 81.8%(54/66), 84.8%(56/66)and 87.9%(58/66), respectively.Conclusion: The complex antigen ESAT-6/CFP-10/Rv3615c has high sensitivity and specificity, and is of certain clinical value for the auxiliary diagnosis of active pulmonary tuberculosis.

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备注/Memo

备注/Memo:
*“十二·五”国家科技重大专项 2013ZX10003002-005; 国家自然科学基金 81271794; 河南省卫生厅科技攻关项目 201204110
#通信作者,男,1956年4月生,硕士,主任医师,研究方向:呼吸系统疾病基础及临床,E-mail:tangbingxiang01@sina.com
更新日期/Last Update: 2017-09-20