[1]童婷婷),马 杰),董丽杰),等.宫颈鳞癌患者宫颈液基脱落细胞中P16、P53蛋白及HPV E6/E7 mRNA的表达[J].郑州大学学报(医学版),2017,(05):637-640.[doi:10.13705/j.issn.1671-6825.2017.05.030]
 TONG Tingting),MA Jie),DONG Lijie),et al.Expressions of P16, P53 proteins and HPV E6/E7 mRNA in cervical liquid-based cells from cervical squanmous cell carcinoma patients[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(05):637-640.[doi:10.13705/j.issn.1671-6825.2017.05.030]
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宫颈鳞癌患者宫颈液基脱落细胞中P16、P53蛋白及HPV E6/E7 mRNA的表达()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年05期
页码:
637-640
栏目:
应用研究
出版日期:
2017-09-15

文章信息/Info

Title:
Expressions of P16, P53 proteins and HPV E6/E7 mRNA in cervical liquid-based cells from cervical squanmous cell carcinoma patients
作者:
童婷婷1)马 杰2)董丽杰1)王 莉1)#
1)郑州大学附属肿瘤医院妇瘤科 郑州 450000 2)郑州大学附属肿瘤医院分子病理科 郑州 450000
Author(s):
TONG Tingting1)MA Jie2)DONG Lijie1)WANG Li1)
1)Department of Female Tumor, the Affiliated Tumor Hospital,Zhengzhou University, Zhengzhou 450000 2)Department of Molecular Pathology, the Affiliated Tumor Hospital,Zhengzhou University,Zhengzhou 450000
关键词:
宫颈鳞癌 P16 P53 HPV E6/E7mRNA 宫颈液基细胞学
Keywords:
cervical squamous cell carcinoma P16 P53 HPV E6/E7 mRNA cervical liquid-based cytology
分类号:
R737.33
DOI:
10.13705/j.issn.1671-6825.2017.05.030
摘要:
目的:探讨宫颈液基脱落细胞中P16、P53蛋白及HPV E6/E7 mRNA的表达情况。方法:采用支链DNA技术及细胞涂片免疫组化的方法检测182例宫颈鳞癌患者宫颈液基脱落细胞中P16、P53蛋白及HPV E6/E7 mRNA的表达水平。结果:宫颈液基脱落细胞中P16表达与淋巴结转移、病理分级有关(P<0.05),P53表达与淋巴结转移、临床分期、病理分级有关(P<0.05),HPV E6/E7 mRNA表达与患者临床分期、淋巴结转移有关(P<0.05); HPV E6/E7 mRNA与P16蛋白表达呈正关联(rP=0.444,P<0.001),与P53蛋白表达不具有关联性(P=0.125); P53蛋白与P16蛋白表达呈正关联(rP=0.505,P<0.001)。结论:宫颈液基脱落细胞中HPV E6/E7 mRNA、P16、P53检测可能对宫颈癌预后评估有重要价值。
Abstract:
Aim: To investigate the value of detection of P16, P53 proteins and HPV E6/E7 mRNA in liquid-based cells of cervical squanmous cell carcinoma patients.Methods: The expressions of HPV E6/E7 mRNA, P16 and P53 proteins were detected by branch-DNA and immunohistochemical method in liquid-based cells from 182 patients with cervical squamous cell carcionma.Results: The expression of HPV E6/E7 mRNA in liquid-based cells was related with lymph node metastasis and clinical stage(P<0.05),the expression of P16 was related with lymph node metastasis and histology classification(P<0.05),and that of P53 was related with lymph node metastasis,clinical stage and histology classification(P<0.05). HPV E6/E7 mRNA in liquid-based cells was positively associated with the expression of P16(rP=0.444,P<0.001),but not with P53(P=0.125). The expression of P53 protein was positively associated with that of P16(rP=0.505,P<0.001).Conclusion: HPV E6/E7 mRNA,P16 and P53 protein detection in liquid-based cells may be valueable for predicting the prognosis of cervical cancer.

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备注/Memo

备注/Memo:
通信作者,女,1964年8月生,学士,主任医师,研究方向:妇科肿瘤的基础与临床,E-mail:2082772078@qq.com
更新日期/Last Update: 2017-09-20