[1]蒋 明,王昌明#,韩旭惠,等.COPD大鼠原代肺动脉平滑肌细胞中MCP-1、TGF-β1和TLR4蛋白的检测*[J].郑州大学学报(医学版),2017,(05):641-644.[doi:10.13705/j.issn.1671-6825.2017.05.031]
 JIANG Ming,WANG Changming,HAN Xuhui,et al.Detection of MCP-1,TGF-β1 and TLR4 protein in primary pulmonary artery smooth muscle cells of COPD rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(05):641-644.[doi:10.13705/j.issn.1671-6825.2017.05.031]
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COPD大鼠原代肺动脉平滑肌细胞中MCP-1、TGF-β1和TLR4蛋白的检测*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年05期
页码:
641-644
栏目:
应用研究
出版日期:
2017-09-15

文章信息/Info

Title:
Detection of MCP-1,TGF-β1 and TLR4 protein in primary pulmonary artery smooth muscle cells of COPD rats
作者:
蒋 明王昌明#韩旭惠杨 丹马礼兵陈 峰吕 倩
桂林医学院附属医院呼吸内科 广西桂林 541001
Author(s):
JIANG MingWANG ChangmingHAN XuhuiYANG DanMA LibingCHEN FengLYU Qian
Department of Respiratory Medicine,Guilin Medical University Affiliated Hospital,Guilin,Guangxi 541001
关键词:
慢性阻塞性肺疾病 Toll样受体4 单核细胞趋化蛋白-1 转化生长因子-β1 大鼠
Keywords:
chronic obstructive pulmonary disease Toll like receptor-4 monocyte chemoattractant protein 1 transforming growth factor β1 rat
分类号:
R563.3
DOI:
10.13705/j.issn.1671-6825.2017.05.031
摘要:
目的:探讨慢性阻塞性肺疾病(COPD)大鼠远端原代肺动脉平滑肌细胞(PASMCs)中单核细胞趋化蛋白-1(MCP-1)、转化生长因子-β1(TGF-β1)的分泌情况及其与Toll样受体4(TLR4)信号通路的相关性。方法:通过脂多糖(LPS)诱导建立大鼠COPD模型,分离培养、鉴定原代大鼠PASMCs,以LPS诱导PASMCs,然后分组(对照组、LPS组、TAK-242组,LPS+TAK-242组)培养细胞,用Western blot法检测各组PASMCs中TLR4的表达,ELISA法检测细胞培养上清液MCP-1、TGF-β1的浓度,并进行相关性分析。结果:LPS组较对照组PASMCs中TLR4的表达水平上调(P<0.05),细胞培养上清液中MCP-1、TGF-β1的含量升高(P<0.05); TAK-242组PASMCs中TLR4的表达水平下调(P<0.05),上清液中MCP-1、TGF-β1与TLR4无相关性。结论:LPS可诱导大鼠原代PASMCs合成分泌MCP-1、TGF-β1,同时上调TLR4表达水平。
Abstract:
Aim: To probe into the secretion of monocyte chemoattractant protein 1(MCP-1),transforming growth factor β1(TGF-β1)in primary pulmonary artery smooth muscle cells(PASMCs)of chronic obstructive pulmonary disease(COPD)rats and the correlation with toll-like receptor 4(TLR4)signaling pathways.Methods: The COPD rat model was established, and the PASMCs were separated, induced by LPS, and then divided into control group,LPS group,TAK-242 group,and LPS+TAK-242 group. The expression of TLR4 in PASMCs of each group was detected by Western blot, and the concentrations of MCP-1 and TGF-β1 in cell culture fluid were determined by ELISA, and at last a correlation analysis was conducted.Results: In contrast with the control group, the expression of TLR4 in PASMCs of the LPS group was inecreased(P<0.05)and the concentrations of MCP-1 and TGF-β1 in cell culture fluid were increased(P<0.05). The expression of TLR4 in PASMCs of the TAK-242 group was down-regulated(P<0.05)and the concentrations of MCP-1 and TGF-β1 in cell culture fluid showed no correlation with the expression of TLR4.Conclusion: LPS can induce PASMCs of rats to synthesize and secrete MCP-1 and TGF-β1, and the TLR4 is up-expressed at the same time.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目 81360010; 广西省卫生厅自然科学基金资助项目 S201603
#通信作者,男,1963年11月生,博士,教授,主任医师,研究方向:慢性阻塞性肺疾病,E-mail:wcm@glmc.edu.cn
更新日期/Last Update: 2017-09-20