[1]郭爱叶),胡金龙),郭 思),等.转染Chk1 siRNA的人胃癌SGC7901细胞对顺铂的敏感性*[J].郑州大学学报(医学版),2017,(06):722-725.[doi:10.13705/j.issn.1671-6825.2017.06.014]
 GUO Aiye),HU Jinlong),GUO Si),et al.Sensitivity of human gastric cancer SGC7901 cells transfected with Chk1 siRNA to cisplatin[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(06):722-725.[doi:10.13705/j.issn.1671-6825.2017.06.014]
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转染Chk1 siRNA的人胃癌SGC7901细胞对顺铂的敏感性*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2017年06期
页码:
722-725
栏目:
应用研究
出版日期:
2017-11-15

文章信息/Info

Title:
Sensitivity of human gastric cancer SGC7901 cells transfected with Chk1 siRNA to cisplatin
作者:
郭爱叶1)胡金龙2)郭 思1)周建炜2)李 刚1)#
1)河南省人民医院检验科 郑州 450003
2)河南省人民医院肿瘤科 郑州 450003
Author(s):
GUO Aiye1)HU Jinlong2)GUO Si1)ZHOU Jianwei2)LI Gang1)
1)Clinical Laboratory,Henan Provincial People's Hospital,Zhengzhou 450003
2)Department of Oncology,Henan Provincial People's Hospital,Zhengzhou 450003
关键词:
细胞周期检测点激酶1 胃癌 RNA干扰 增殖 凋亡
Keywords:
checkpoint kinases 1 gastric neoplasm RNA interference proliferation apoptosis
分类号:
R735.2
DOI:
10.13705/j.issn.1671-6825.2017.06.014
摘要:
目的:探讨Chk1 siRNA对顺铂作用的人胃癌SGC7901细胞增殖及凋亡的影响。方法:构建3个Chk1 siRNA重组载体,分组转染人胃癌SGC7901细胞,采用实时荧光定量PCR方法检测细胞中Chk1 mRNA的表达,应用Western blot检测Chk1蛋白的表达。以效应最强的Chk1 siRNA转染胃癌SGC7901细胞24 h,再用终浓度为10 μmol/L的顺铂处理12 h(Chk1 S2+顺铂组)。运用MTT法检测细胞增殖抑制率,TUNEL法检测细胞凋亡。以顺铂处理和未经任何处理的细胞作对照。结果:3条Chk1 siRNA转染SGC7901细胞后,Chk1 mRNA及蛋白表达减弱(P<0.05),以Chk1 S2效应最佳。Chk1 S2+顺铂组SGC7901细胞的增殖抑制率和凋亡指数(AI)均高于顺铂组及对照组(P<0.05)。结论:体外沉默Chk1表达可增强人胃癌SGC7901细胞对顺铂的敏感性。
Abstract:
Aim: To investigate the influence of cisplatin on proliferation and apoptosis of human gastric cancer SGC7901 cells transfected with Chk1 siRNA.Methods: Three kinds of Chk1 siRNA were established and transfected into SGC7901 cells. Real-time PCR and Western blot were used to detect the expressions of Chk1 mRNA and protein in SGC7901 cells, respectively. The Chk1 siRNA with strongest inhibitory effect on Chk1 expression was transfected into SGC7901 cells for 24 h, then treated by cisplatin(10 μmol/L)for 12 h, MTT method was used to detect the cell proliferation, and TUNEL method was used to detect cell apoptosis. The cells treated with cisplatin alone and without any treatment were the controls.Results: Chk1 protein and mRNA expression levels in SGC7901 cells transfected with Chk1 mRNA both decreased(P<0.05), and the inhibition effect of Chk1 S2 was stronger. The cell proliferation inhibition rate and apoptosis index in Chk1 S2+cisplatin group were higher than those in the 2 control groups(P<0.05).Conclusion: Silencing Chk1 expression might enhance the sensitivity of SGC7901 cells to cisplatin.

参考文献/References:

[1] 郑林峰,倪型灏.细胞周期检测点激酶Ⅰ研究进展[J].浙江实用医学,2016,21(1):76
[2] OZA V,ASHWELL S,BRASSIL P,et al.Synthesis and evaluation of triazolones as checkpoint kinase 1 inhibitors[J].Bioorg Med Chem Lett,2012,22(6):2330
[3] WANG WT,CATTO J,MEUTH M.Differential response of normal and malignant urothelial cells to CHK1 and ATM inhibitors[J].Mol Cancer Ther,2013,12(11, S):2887
[4] BUISSON R,BOISVERT JL,BENES CH,et al.Distinct but concerted roles of ATR, DNA-PK, and Chk1 in countering replication stress during S phase[J].Mol Cell,2015,59(6):1011
[5] 卢创新,罗执芬,周建炜,等.黄芪多糖通过激活线粒体凋亡途径诱导食管癌EC9706细胞的凋亡并增加其对顺铂的敏感性[J].中华实验外科杂志,2013,30(3):539
[6] GOTO H,IZAWA I,LI P,et al.Novel regulation of checkpoint kinase 1(Chk1):is Chk1 a good candidate for anti-cancer therapy[J].Cancer Sci,2012,103(7):1195
[7] RYCAI K,TANG DG.Cancer stem cells and radioresistance[J].Int J Radiat Biol,2014,90(8):615
[8] PARPLYS AC,PETERMANN E,PETERSEN C,et al.DNA damage by X-rays and their impact on replication processes[J].Radiother Oncol,2012,102(3):466
[9] BRYANT C,STOKES S,MASSEY AJ.Chk1 inhibition as a novel therapeutic strategy for treating triple negative breast and ovarian cancers[J].Cancer Res,2011,71(8):570
[10]刘艳杰,顾浩,乔玉环,等.沉默细胞周期检测点激酶1(Chk1)对宫颈癌HeLa细胞放射敏感性的影响[J].中华放射医学与防护杂志,2017,37(2):102
[11]孙冬岩,王燕,姚冬梅,等.Chk1基因沉默对人卵巢癌细胞 A2780放疗敏感性的影响[J].山东医药,2014,54(13):6

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备注/Memo

备注/Memo:
*河南省科技攻关计划项目 142102310392; 河南省医学科技攻关计划项目 201304045
#通信作者,男,1965年6月生,本科,副主任技师,研究方向:肿瘤检验,E-mail:13803852016@163.com
更新日期/Last Update: 2017-11-20