[1]杨俊强,胡晓辰,齐义军,等.贲门癌中长链非编码RNA和微小RNA差异表达谱及相关性分析[J].郑州大学学报(医学版),2018,(01):10-13.[doi:10.13705/j.issn.1671-6825.2017.03.043]
 YANG Junqiang,HU Xiaochen,QI Yijun,et al.Differential expression profiles and correlation analysis of lncRNA and miRNA in gastric cardia adenocarcinoma[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(01):10-13.[doi:10.13705/j.issn.1671-6825.2017.03.043]
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贲门癌中长链非编码RNA和微小RNA差异表达谱及相关性分析()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年01期
页码:
10-13
栏目:
食管癌研究
出版日期:
2018-01-20

文章信息/Info

Title:
Differential expression profiles and correlation analysis of lncRNA and miRNA in gastric cardia adenocarcinoma
作者:
杨俊强胡晓辰齐义军高社干
河南科技大学医学院临床医学院; 河南科技大学第一附属医院肿瘤医院; 河南省肿瘤表观遗传重点实验室 河南洛阳 471003
Author(s):
YANG Junqiang HU Xiaochen QI YijunGAO Shegan
College of Clinical Medicine, Medical College, Henan University of Science and Technology; Cancer Hospital, the First Affiliated Hospital of Henan University of Science and Technology; Henan Key Laboratory of Cancer Epigenetics, Luoyang, Henan 471003
关键词:
贲门癌 基因芯片 生物信息预测 长链非编码RNA 微小RNA
Keywords:
gastric cardia adenocarcinoma microarray bioinformatics prediction lncRNA miRNA
分类号:
R735.2
DOI:
10.13705/j.issn.1671-6825.2017.03.043
摘要:
目的:分析贲门癌与癌旁组织中长链非编码RNA(lncRNA)和微小RNA(miRNA)的差异表达谱及二者的相关性。方法:利用基因芯片技术鉴定15例贲门癌与癌旁组织中的lncRNA及miRNA的差异表达谱,生物信息方法分析与H19相互作用的候选miRNA分子,qRT-PCR验证H19和miR-148a-3p在贲门癌中表达的相关性。结果:15例贲门癌与癌旁组织中鉴定出5倍以上差异性表达的lncRNA 182个,2倍以上差异性表达的miRNA 152个。生物信息学预测结果表明,lncRNA H19与7个miRNA表达具有相关性。qRT-PCR验证37例贲门癌与癌旁组织中H19和miR-148a-3p的表达呈负相关(r=-0.398,P<0.001)。结论:贲门癌中差异表达的lncRNA和miRNA分子参与了癌变过程,H19可能直接调控miR-148a-3p的表达。
Abstract:
Aim: To analyze the expression profiles and correlations of long non-coding RNA(lncRNA)and microRNA(miRNA)in gastric cardia adenocarcinoma(GCA)and surrounding tissue.Methods: Using microarray technology, the expression of lncRNA and miRNA in 15 pairs of GCA and matched surrounding mucosa tissue samples were profiled. The correlations between the expressions of lncRNA and miRNA were evaluated by bioinformatics approach, followed by qRT-PCR to verify the correlations.Results: A total of lncRNAs(>5-fold difference)and 152 miRNAs(>2-fold difference)were identified in 15 GCA samples compared with their counterparts. Among these 152 differential miRNAs, 7 miRNA candidates were predicted to be targets of lncRNA H19. In another independent cohort analysis of 37 GCA cases, there was negative correlation between expression of H19 and miR-148a-3p validated by qRT-PCR(r=-0.398, P<0.001).Conclusion: Differentially expressed lncRNAs and miRNAs are implicated in carcinogenesis of gastric cardia. LncRNA H19 may directly modulate the expression of miR-148a-3p.

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备注/Memo

备注/Memo:
【基金项目】国家自然科学基金资助项目(81472234)
【作者简介】高社干,通信作者,男,1968年10月生,博士,主任医师,研究方向:消化道肿瘤的基础与临床,E-mail:gsg112258@163.com
更新日期/Last Update: 2018-01-20