[1]黄 倩,柳懿鹏.不同压力灌注下上尿路梗阻大鼠的肾损伤程度[J].郑州大学学报(医学版),2018,(01):117-121.[doi:10.13705/j.issn.1671-6825.2017.06.063]
 HUANG Qian,LIU Yipeng.Acute nephron injury degree in rats with upper urinary tract obstruction and different pressure perfusion[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(01):117-121.[doi:10.13705/j.issn.1671-6825.2017.06.063]
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不同压力灌注下上尿路梗阻大鼠的肾损伤程度()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年01期
页码:
117-121
栏目:
应用研究
出版日期:
2018-01-20

文章信息/Info

Title:
Acute nephron injury degree in rats with upper urinary tract obstruction and different pressure perfusion
作者:
黄 倩柳懿鹏
武汉市第一医院泌尿外科 武汉 430022
Author(s):
HUANG QianLIU Yipeng
Department of Urology,the No.1 Hospital of Wuhan,Wuhan 430022
关键词:
上尿路梗阻 肾盂高压灌注 肾损伤 大鼠
Keywords:
upper urinary tract obstruction high-pressure perfusion of renal pelvis nephron injury rat
分类号:
R692
DOI:
10.13705/j.issn.1671-6825.2017.06.063
摘要:
目的:观察不同压力灌注下上尿道梗阻大鼠肾损伤情况。方法:将80只SD大鼠随机分为8组,每组10只。 M0~M3组制备轻度上尿路梗阻模型并分别给予0、20、60、100 mmHg压力的肾盂灌注,S0~S3组制备重度上尿路梗阻模型并分别给予相应压力的肾盂灌注。灌注8 min后停止2 min,重复操作4次。术后48 h收集梗阻侧肾组织,检测肾小管上皮细胞凋亡指数,Western blot法检测肾损伤分子1(KIM-1)和富半胱氨酸蛋白61(Cyr61)的表达。结果:梗阻程度越重,灌注压力越高,肾小管上皮细胞凋亡指数越大,KIM-1和Cyr61的表达越强(P<0.05)。结论:上尿路梗阻降低了导致肾损伤的肾盂灌注压力阈值。
Abstract:
Aim: To observe the degree of acute nephron injury in rats with upper urinary tract obstruction and different pressure perfusion.Methods: A total of 80 SD rats were equally divided into 8 groups(n=10). The rats in groups M0,M1,M2 and M3 received an operation to induce mild hydronephrosis model and then were perfused with 0,20,60,and 100 mmHg pressure fluid, respectively. The rats in groups S0,S1,S2 and S3 were made severe hydronephrosis model and then were perfused with corresponding pressure fluid. The operation was 8 min-perfusion and then 2 min-intermittent, and repeated 4 times. 48 h after the perfusion, the possible change of pathology in kidney tissue was investigated, the apoptosis index was detected, and the expression levels of kidney injury molecule 1(KIM-1)protein and cysteine-rich protein 61(Cyr61)were detected by Western blot.Results: The apoptosis index and the expression levels of KIM-1 and Cyr61 increased with the increase of hydronephrosis degree and perfusion pressure(P<0.05).Conclusion: Upper urinary tract obstruction may reduce the threshold of kidney perfusion pressure which could induce acute kidney injury.

参考文献/References:

[1] AYDIN M,HAKAN N,DEVECI U,et al.Comment on the paper entitled 'Erythropoietin ameliorates oxidative stress and tissue injury following renal ischemia/reperfusion in rat kidney and lung'[J].Med Princ Pract,2014,23(5):485
[2] 曾鹏,蒋重和,莫鉴锋,等.新型标准肾镜联合EMS第四代碎石清石系统经皮肾镜取石术中肾盂内压变化[J].现代泌尿外科杂志,2016,21(10):768
[3] ZHANG S,ZHANG Q,JI C,et al.Improved split renal function after percutaneous nephrostomy in young adults with severe hydronephrosis due to ureteropelvic junction obstruction[J].J Urol,2015,193(1):191
[4] 赵伟,赵永哲,王君,等.输尿管硬镜联合输尿管软镜治疗嵌顿性输尿管上段结石126例临床效果评价[J].实用临床医药杂志,2016,20(7):140
[5] CHRISTENSEN ME,JANSEN ES,SANCHEZ W,et al.Flow cytometry based assays for the measurement of apoptosis-associated mitochondrial membrane depolarisation and cytochrome c release[J].Methods,2013,61(2):138
[6] 曹全富,王洛夫,兰卫华,等.C-反应蛋白检测在上尿路结石合并肾脏化脓性感染诊治中的应用价值[J].检验医学与临床,2016,13(21):3091
[7] 刘刚,唐瑭,谭凡,等.肾静态显像诊断上尿路感染的临床研究[J].现代生物医学进展,2016,16(6):1124
[8] 史红雷,郭宏骞,李笑弓,等.肾结石患者行MPCNL中肾盂内压的测定及其临床意义[J].江苏医药,2007,33(6):612
[9] 邓飞,许文德,姚子明,等.经皮肾镜取石术在老年复杂性肾结石中的应用[J].中国老年学杂志,2016,36(19):4832
[10]JANSSEN C,JÄGER W,MOSKALEV I,et al.Erythropoietin accelerates the regeneration of ureteral function in a murine model of obstructive uropathy[J].J Urol,2015,193(2):714
[11]柳懿鹏,曹智修.肾衰合剂对大鼠肾盂高灌注压损伤的保护作用[J].中成药,2017,39(3):608
[12]SALERNO A,NAPPO SG,MATARAZZO E,et al.Treatment of pediatric renal stones in a Western country: a changing pattern[J].J Pediatr Surg,2013,48(4):835

备注/Memo

备注/Memo:
【作者简介】柳懿鹏,通信作者,男,1978年1月生,硕士,主治医师,研究方向:泌尿系统结石及前列腺疾病的诊治,E-mail:lypans@126.com
更新日期/Last Update: 2018-01-20