[1]路富林),余 琦),魏 明).运动预处理对大鼠心脏缺血再灌注损伤的保护作用[J].郑州大学学报(医学版),2018,(03):323-327.[doi:10.13705/j.issn.1671-6825.2017.09.040]
 LU Fulin),YU Qi),WEI Ming).Effect of exercise preconditioning on myocardial ischemia reperfusion injury in rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(03):323-327.[doi:10.13705/j.issn.1671-6825.2017.09.040]
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运动预处理对大鼠心脏缺血再灌注损伤的保护作用()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年03期
页码:
323-327
栏目:
应用研究
出版日期:
2018-03-20

文章信息/Info

Title:
Effect of exercise preconditioning on myocardial ischemia reperfusion injury in rats
作者:
路富林1)余 琦2)魏 明2)
1)西安医学院体育部 西安 710021;2)西安医学院基础医学部 西安 710021
Author(s):
LU Fulin1)YU Qi2)WEI Ming2)
1)Department of Physical Education,Xi'an Medical University, Xi'an 710021;2)School of Basic Medical Sciences, Xi'an Medical University, Xi'an 710021
关键词:
运动预处理 缺血再灌注损伤 心脏 Sirt1 大鼠
Keywords:
exercise preconditioning ischemia reperfusion injury heart Sirt1 rat
分类号:
G804.2
DOI:
10.13705/j.issn.1671-6825.2017.09.040
摘要:
目的:探讨运动预处理对心脏缺血再灌注损伤的保护作用及可能机制。方法:雄性成年SD大鼠40只,随机分为4组,每组10只:对照组、运动预处理组、缺血再灌注组、运动预处理+缺血再灌注组,运动预处理组、运动预处理+缺血再灌注组进行6周的中等强度跑台运动。建立在体心脏缺血再灌注(缺血30 min,再灌注120 min)模型; 检测血清中cTnI、LDH的含量,HE染色观察心肌纤维结构的变化,Western blot检测心肌组织中Cleaved Caspase-3、Cytochrome C、Sirt1和Ac-FOXO1蛋白的表达,测定心肌线粒体SOD活性和MDA含量。结果:运动预处理组和对照组相比,各项指标差异均无统计学意义(P均>0.05); 心肌缺血再灌注后,血清中LDH和cTnI含量升高,心肌纤维结构出现断裂,凋亡相关蛋白Cleaved Caspase-3、Cytochrome C表达增加,SOD活性下降,MDA含量增加,Sirt1蛋白表达减少,FOXO1乙酰化增加(P均<0.05); 而运动预处理可明显改善心肌缺血再灌注引起的LDH和cTnI升高,减少心肌纤维断裂,下调凋亡相关蛋白Cleaved Caspase-3、Cytochrome C的表达,增加SOD活性,降低MDA含量,上调Sirt1蛋白的表达,减少FOXO1的乙酰化(P均<0.05)。结论:运动预处理对心肌缺血再灌注损伤具有保护作用,这种保护作用可能是通过上调Sirt1的表达,从而降低心肌氧化应激损伤实现的。
Abstract:
Aim: To investigate the role and underlying mechanism of exercise preconditioning(EP)on myocardial ischemia reperfusion(IR).Methods: Forty male SD rats were randomly allocated into 4 groups(n=10), namely the control, EP, IR, EP+IR groups. The rats in EP and EP+IR groups were conducted middle intensity exercises for six weeks, the ischemia(30 min)reperfusion(120 min)model was induced by occlusion of left anterior descending branch of coronary artery. Serum lactate dehydrogenase(LDH)and cTnI were determined. The morphological changes in myocardial fiber were analyzed by HE staining. Western blot was used to determine the expressions of Cleaved Caspase-3, Cytochrome C, Sirt1 and Ac-FOXO1. The activity of superoxide dismutase(SOD)and the content of malondialdehyde(MDA)were measured.Results: Compared with control group, EP had no effect on normal hearts(P>0.05). The hearts in IR group exhibited increases in the content of LDH, cTnI, MDA, the expression of Cleaved Caspase-3, Cytochrome C, and the expression of Ac-FOXO1,and decreases in the activity of SOD and the expression of Sirt1(P<0.05). The hearts of EP+IR group were significantly attenuated the above effects induced by IR(P<0.05).Conclusion: EP could alleviate IR-induced heart injury partly via upregulating Sirt1 and finally reduce the oxidative stress injury.

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备注/Memo

备注/Memo:
【作者简介】路富林,通信作者,男,1983年1月生,硕士,讲师,研究方向:运动医学与康复训练,E-mail:16707705@qq.com
更新日期/Last Update: 2018-05-20