[1]李君君,方 磊,高 阳,等.Septin2在胃癌组织中的表达及对胃癌细胞增殖和凋亡的影响[J].郑州大学学报(医学版),2018,(03):332-337.[doi:10.13705/j.issn.1671-6825.2017.11.012]
 LI Junjun,FANG Lei,GAO Yang,et al.Expression of Septin2 and its effect on cell proliferation and apoptosis in gastric cancer[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(03):332-337.[doi:10.13705/j.issn.1671-6825.2017.11.012]
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Septin2在胃癌组织中的表达及对胃癌细胞增殖和凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年03期
页码:
332-337
栏目:
应用研究
出版日期:
2018-03-20

文章信息/Info

Title:
Expression of Septin2 and its effect on cell proliferation and apoptosis in gastric cancer
作者:
李君君方 磊高 阳时 帅何昊宇刘晓梅袁彩君
锦州医科大学附属第一医院肿瘤内科 辽宁锦州 121000
Author(s):
LI Junjun FANG Lei GAO Yang SHI Shuai HE Haoyu LIU Xiaomei YUAN Caijun
Department of Oncology, the First Affiliated Hospital, Jinzhou Medical University, Jinzhou, Liaoning 121000
关键词:
Septin2 基因芯片 胃癌
Keywords:
Septin2 microarray gastric cancer
分类号:
R735.2
DOI:
10.13705/j.issn.1671-6825.2017.11.012
摘要:
目的:研究GTP结合蛋白氯苄乙胺2(Septin2)在胃癌组织中的表达及对胃癌细胞增殖、凋亡的影响。方法:对基因表达综合数据库(GEO)中GSE54129数据集的基因芯片进行分析,比较胃正常组织和癌组织中Septin2 mRNA的表达差异; 利用Kaplan-Meier Plotter数据库进行生存分析,明确Septin2表达与胃癌预后的关系。收集胃正常和癌组织标本进行免疫组化染色,分析Septin2蛋白表达的差异。用Septin2基因表达质粒转染胃癌SCG-7901和MNK-45细胞,观察细胞增殖和凋亡情况。结果:胃癌组织中Septin2 mRNA和蛋白表达水平均高于正常组织(P<0.05),Septin2 mRNA表达水平与胃癌预后相关,Septin2 mRNA高表达者中位生存时间短于低表达者(P<0.001)。与空白对照组和阴性对照组相比,转染Septin2基因的SCG-7901和MNK-45细胞Septin2表达增加,细胞增殖能力增强,凋亡能力下降(P<0.001)。结论:Septin2基因过表达可促进胃癌细胞的增殖,抑制其凋亡; 与胃癌预后关系密切。
Abstract:
Aim: To investigate the Septin2 expression in gastric carcinoma tissue and its effect on proliferation and apoptosis of gastric cancer cells.Methods: The Septin2 mRNA expression in gastric cancer tissue and normal tissue from GSE54129 data set of GEO database were compared, and survival analysis was performed using the Kaplan-Meier Plotter database.The expressions of Septin2 protein in gastric normal tissue and cancer tissue were analyzed by immunohistochemical staining. The SCG-7901 and MNK-45 cell lines were transfected with the plasmid containing Septin2 gene, and then cell proliferation and apoptosis were analyzed.Results: The expression of Septin2 in gastric carcinoma tissue was significantly higher than that in normal tissue at mRNA level and protein level(P<0.001). Septin2 mRNA expression was associated with prognosis, and median survival time of patients with high Septin2 mRNA expression was shorter than that with low expression(P<0.001). The cells transfected Septin2 gene had higher proliferation abilities and lower apoptosis abilities(P<0.001).Conclusion: The overexpression of Septin2 gene can promote the proliferation and inhibit the apoptosis of gastric cancer cells. Septin2 is closely related to the prognosis of gastric cancer.

参考文献/References:

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备注/Memo

备注/Memo:
【基金项目】辽宁省科学技术计划项目(2010010280-401)
【作者简介】袁彩君,通信作者,女,1960年3月生,本科,主任医师,教授,研究方向:乳腺癌的临床诊断及生物治疗,E-mail:2151208698@qq.com
更新日期/Last Update: 2018-05-20