[1]郭振江),刘宗文),郑瑞锋),等.沉默单羧酸转运体4对宫颈癌Hela、Siha细胞增殖、侵袭迁移及凋亡的影响[J].郑州大学学报(医学版),2018,(03):354-359.[doi:10.13705/j.issn.1671-6825.2017.10.051]
 GUO Zhenjiang),LIU Zongwen),ZHENG Ruifeng),et al.Effect of silencing monocarboxylate transporter 4 on proliferation, invasion, migration and apoptosis of cervical cancer cells,Hela and Siha[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(03):354-359.[doi:10.13705/j.issn.1671-6825.2017.10.051]
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沉默单羧酸转运体4对宫颈癌Hela、Siha细胞增殖、侵袭迁移及凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年03期
页码:
354-359
栏目:
应用研究
出版日期:
2018-03-20

文章信息/Info

Title:
Effect of silencing monocarboxylate transporter 4 on proliferation, invasion, migration and apoptosis of cervical cancer cells,Hela and Siha
作者:
郭振江1)刘宗文1)郑瑞锋2)宋 锐1)张 艳3)楚阿兰1)柴 婷1)刘耀河1)侯 歌1)尚丰阔4)
1)郑州大学第二附属医院放射治疗科 郑州 450014;2)武警河南省总队医院肿瘤血液科 郑州 450052;3)郑州大学基础医学院药理学教研室 郑州 450001;4)中国人民解放军69223部队 新疆阿克苏 842300
Author(s):
GUO Zhenjiang1) LIU Zongwen1) ZHENG Ruifeng2) SONG Rui1) ZHANG Yan3) CHU Alan1) CHAI Ting1) LIU Yaohe1) HOU Ge1)SHANG Fengkuo4)
1)Department of Radiotherapy, the Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014; 2)Department of Oncology and Hematology, Armed Police Corps Hospital of Henan Province, Zhengzhou 450052;3)Department of Pharmacology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001;4)Chinese People's Liberation Army 69223, Akesu, Xinjiang 842300
关键词:
单羧酸转运体4 宫颈癌 乳酸 肿瘤代谢重编程 基因沉默
Keywords:
monocarboxylate transporter 4 cervical cancer lactic acid tumor metabolism reprogramming gene silencing
分类号:
R737.33
DOI:
10.13705/j.issn.1671-6825.2017.10.051
摘要:
目的:探讨沉默单羧酸转运体4(MCT4)基因对宫颈癌Hela、Siha细胞增殖、侵袭迁移及凋亡的影响。方法:通过瞬时转染siRNA沉默Hela、Siha细胞的MCT4基因,Western blot筛选出沉默效果较好的siMCT4序列进行后续实验。转染后分别用乳酸试剂盒检测各组细胞外液中乳酸浓度的变化,CCK8实验检测各组细胞增殖,划痕实验检测各组细胞迁移,Transwell侵袭实验检测细胞侵袭能力,流式细胞术检测细胞凋亡情况。结果:Western blot显示siMCT4#2沉默Hela和Siha中的MCT4效果更好; 与空白对照组和阴性对照相比,siMCT4#2转染Hela、Siha 后,实验组(siMCT4#2)细胞外液乳酸浓度(P<0.05)、细胞增殖率(P<0.05)及细胞迁移率(P<0.001)、侵袭率(P<0.05)均下降,而细胞凋亡率上升(P<0.001)。结论:沉默Hela和Siha的MCT4可抑制其乳酸的外排,抑制两种细胞系的增殖、侵袭、迁移,促进其凋亡。
Abstract:
Aim: To investigate the effects of silencing monocarboxylate transporter 4(MCT4)geneon proliferation, invasion, migration and apoptosis of cervical cancer cells, Hela and Siha.Methods: The MCT4 gene of Hela and Siha cells were silenced by transient transfection of siRNA, and the siMCT4 sequence with good silencing effect was screened by Western blot for follow-up experiments. After the transfection, the lactic acid concentration in the extracellular fluid of each group was detected by the lactic acid kit. The cell proliferation of each group was detected by the CCK8 experiment. The cell migration was detected by the scratch test, and the invasion ability of the cells was detected by the Transwell invasion experiment. Apoptosis was detected by flow cytometry.Results: Western blot showed that the effect of siMCT4#2 was better in silencing MCT4 of Hela and Siha. Compared with the blank control group and the negative control group, after transfected with siMCT4#2 in Hela and Siha, the concentration of lactic acid in extracellular fluid, the cell migration rate, the invasion rate and cell proliferation rate all decreased in experimental group(siMCT4#2)(P<0.05), but the cell apoptosis rate increased(P<0.001).Conclusion: Silencing MCT4 gene in Hela and Siha can inhibit the efflux of lactic acid, inhibit the proliferation, invasion and migration of two cell lines and promote their apoptosis.

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备注/Memo

备注/Memo:
【基金项目】河南省基础与前沿技术研究计划项目(132300410409); 河南省医学科技攻关计划项目(201401009); 国家重点研发计划数字诊 疗装备研发重点专项(2016YFC0105713)
【作者简介】刘宗文,男,通信作者,1966年12月生,博士,主任医师,教授,研究方向:肿瘤的放射治疗,E-mail:liuzwhh@sina.com
更新日期/Last Update: 2018-05-20