[1]陈亚利),欧阳军),孟晶茜),等.姜黄素对高尿酸血症大鼠肾组织中TGF-β1、NF-κB表达的影响[J].郑州大学学报(医学版),2018,(03):360-364.[doi:10.13705/j.issn.1671-6825.2017.11.033]
 CHEN Yali),OUYANG Jun),MENG Jingqian),et al.Effects of curcumin on TGF-β1 and NF-κB in renal tissue of rats with hyperuricemia[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(03):360-364.[doi:10.13705/j.issn.1671-6825.2017.11.033]
点击复制

姜黄素对高尿酸血症大鼠肾组织中TGF-β1、NF-κB表达的影响()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年03期
页码:
360-364
栏目:
应用研究
出版日期:
2018-03-20

文章信息/Info

Title:
Effects of curcumin on TGF-β1 and NF-κB in renal tissue of rats with hyperuricemia
作者:
陈亚利1)欧阳军2)孟晶茜1)刘 丁1)张文吉1)崔琴琴1)赵瑛瑛1)
1)郑州大学第二附属医院肾内科 郑州 450014;2)郑州大学医药科学研究院内分泌实验室 郑州 450052
Author(s):
CHEN Yali1)OUYANG Jun2)MENG Jingqian1)LIU Ding1)ZHANG Wenji1)CUI Qinqin1)ZHAO Yingying1)
1)Department of Nephrology, the Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014;2)Endocrine Laboratory,Institute of Medical and Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450052
关键词:
高尿酸血症 转化生长因子-β1 核因子-κB 姜黄素 大鼠
Keywords:
hyperuricemia TGF-β1 NF-κB curcumin rat
分类号:
R589.7
DOI:
10.13705/j.issn.1671-6825.2017.11.033
摘要:
目的:观察姜黄素对高尿酸血症(HUA)大鼠肾组织中转化生长因子-β1(TGF-β1)、核因子-κB(NF-κB)表达的影响。方法:SD大鼠36只,随机分为正常对照组、氧嗪酸钾组和氧嗪酸钾+姜黄素组(联合组),每组12只,氧嗪酸钾灌胃制备HUA模型,模型成功后联合组给予姜黄素灌胃治疗8周。检测大鼠血尿酸(UA)、肌酐(Scr)、尿素氮(BUN)和24 h尿蛋白定量及血清胱抑制素C(Cys-C)水平; HE染色及Masson染色观察肾脏病理变化情况,免疫组织化学法检测肾组织中TGF-β1、NF-κB蛋白的表达。结果:与对照组比较,氧嗪酸钾组及联合组UA、Scr、BUN、24 h尿蛋白定量、Cys-C水平均升高(P<0.05); 联合组较氧嗪酸钾组血清BUN、Scr、24 h尿蛋白定量、Cys-C均下降(P<0.05); HE染色显示氧嗪酸钾组肾小管炎性细胞浸润、肾小管轻度扩张,联合组较氧嗪酸钾组肾小管损伤程度减轻; Masson染色显示氧嗪酸钾组肾小管间质纤维化程度大于对照组及联合组,联合组纤维化程度减轻; 免疫组化检测结果显示氧嗪酸钾组TGF-β1 、NF-κB表达水平升高(P<0.05),联合组TGF-β1 、NF-κB表达量较氧嗪酸钾组下降(P<0.05)。结论:姜黄素对HUA肾脏具有保护作用,其机制可能与下调TGF-β1、NF-κB的表达有关。
Abstract:
Aim: To observe the effect of curcumin on the expressions of TGF-β1 and NF-κB in renal tissue of rats with hyperuricemia(HUA).Methods: Thirty-six male SD rats were randomly allocated into three groups: control group(NC)group, potassium oxonate(OP)group, and potassium oxonate with curcumin group(OC)group, with 12 rats in each group. Sodium carboxymethy cellulose solution at 5 g/L containing OP was used to establish hyperuricemia model by gastric gavage once per day. OC group was treated with curcumin at the same time.NC group was treated with 10 g/L sodium carboxymethy cellulose only.The levels of serum uric acid(UA), creatinine(Scr), urea nitrogen(BUN), 24-h urinary protein and cystatin C(Cys-C)were measured at 12th week.The histological changes of kidneys were observed via HE staining and Masson staining by light microscopy.The expressions of TGF-β1 and NF-κB in renal tissue were examined by immunohistochemistry staining.Results: Compared with NC group, the values of UA, Scr, BUN, 24-h urinary protein and Cys-C were higher in OP group and OC group(P<0.05); compared with OP group, the values of BUN,Scr, 24-h urinary protein and Cys-C in the OC group were lower at 12th week(P<0.05). HE and Masson staining showed that OC group had less damage and tubulointestitial fibrosis compared with OP group. The expressions of TGF-β1 and NF-κB in the glomeruli and renal tubules of the OP group were significantly higher than those in the control group(P<0.05). The expression of NF-κB,TGF-β1 was significantly down-regulated in OC group than that in OP group(P<0.05).Conclusion: The expressions of TGF-β1 and NF-κB in renal tissue of rats with HUA was up-regulated, and curcumin can protect the kidney by down-regulating the expressions of TGF-β1 and NF-κB in HUA.

参考文献/References:

[1] ROMI MM, ARFIAN N, TRANGGONO U, et al. Uric acid causes kidney injury through inducing fibroblast expansion, Endothelin-1 expression, and inflammation[J]. BMC Nephrol, 2017, 18(1): 326
[2] HONG Q, YU S, GENG X, et al. High concentrations of uric acid inhibit endothelial cell migration via miR-663 which regulates phosphatase and tensin homolog by targeting transforming growth factor-β1[J]. Microcirculation, 2015, 22(4): 306
[3] 林章梅,张荣山,范晨雪,等.非布司他对高尿酸血症大鼠肾小管上皮间质转化及血清IL-6、转化生长因子β1的影响[J].中华内科杂志,2017,56(5):338
[4] GUIJARRO C, EGIDO J. Transcription factor-kappa B(NF-kappa B)and renal disease[J]. Kidney Int, 2001, 59(2): 415
[5] ZHOU Y, FANG L, JIANG L, et al. Uric acid induces renal inflammation via activating tubular NF-κB signaling pathway[J]. PLoS One, 2012, 7(6): e39738
[6] LIU H, XIONG J, HE T, et al. High uric acid-induced epithelial-mesenchymal transition of renal tubular epithelial cells via the TLR4/NF-κB signaling pathway[J]. Am J Nephrol, 2017, 46(4): 333
[7] ALI BH, AL-SALAM S, AL SULEIMANI Y, et al. Curcumin ameliorates kidney function and oxidative stress in experimental chronic kidney disease[J]. Basic Clin Pharmacol Toxicol, 2018, 122(1): 65
[8] SÁNCHEZ-LOZADA LG, TAPIA E, SOTO V, et al. Treatment with the xanthine oxidase inhibitor febuxostat lowers uric acid and alleviates systemic and glomerular hypertension in experimental hyperuricaemia[J]. Nephrol Dial Transplant, 2008, 23(4): 1179
[9] MAZZALI M, HUGHES J, KIM YG, et al. Elevated uric acid increases blood pressure in the rat by a novel crystal-independent mechanism[J]. Hypertension, 2001, 38(5): 1101
[10]SANCHEZ-LOZADA LG, SOTO V, TAPIA E,et al. Role of oxidative stress in the renal abnormalities induced by experimental hyperuricemia[J].Am J Physiol Renal Physiol,2008,295(4):F1134
[11]CHOI YJ, YOON Y, LEE KY, et al. Uric acid induces endothelial dysfunction by vascular insulin resistance associated with the impairment of nitric oxide synthesis[J]. FASEB J, 2014, 28(7): 3197
[12]SANCHEZ-LOZADA LG, TAPIA E, SANTAMAra J, et al. Mild hyperuricemia induces vasoconstriction and maintains glomerular hypertension in normal and remnant kidney rats [J].Kidney Int,2005,67(1):237
[13]ASAKAWA S, SHIBATA S, MORIMOTO C, et al. Podocyte injury and albuminuria in experimental hyperuricemic model rats[J]. Oxid Med Cell Longev, 2017, 2017: 3759153
[14]AGGARWAL BB, SUNG B. Pharmacological basis for the role of curcumin in chronic diseases: an age-old spice with modern targets[J]. Trends Pharmacol Sci, 2009, 30(2): 85
[15]杨瑞凤,王建生,张宛哲, 等.活性维生素 D3对糖尿病大鼠肾脏 BMP-7及 TGF-β1表达的影响[J].郑州大学学报(医学版),2013,48(5):616
[16]徐静,易兰兰,张春虹, 等.二酯酰甘油-蛋白激酶C信号传导系统和细胞因子与糖尿病肾病的关系[J].西安交通大学学报(医学版),2006,27(2):168
[17]ZEISBERG M, DUFFIELD JS. Resolved: EMT produces fibroblasts in the kidney[J]. J Am Soc Nephrol, 2010, 21(8): 1247
[18]DERYNCK R, ZHANG YE. Smad-dependent and Smad-independent pathways in TGF-beta family signalling[J]. Nature, 2003, 425(6958): 577
[19]ZHU FQ, CHEN MJ, ZHU M, et al. Curcumin suppresses epithelial-mesenchymal transition of renal tubular epithelial cells through the inhibition of Akt/mTOR pathway[J]. Biol Pharm Bull, 2017, 40(1): 17
[20]HO C, HSU YC, LEI CC, et al. Curcumin rescues diabetic renal fibrosis by targeting superoxide-mediated Wnt signaling pathways[J]. Am J Med Sci, 2016, 351(3): 286
[21]CHIU J, KHAN ZA, FARHANGKHOEE H, et al. Curcumin prevents diabetes-associated abnormalities in the kidneys by inhibiting p300 and nuclear factor-kappaB[J]. Nutrition, 2009, 25(9): 964
[22]ZHANG DM, LI YC, XU D, et al. Protection of curcumin against fructose-induced hyperuricaemia and renal endothelial dysfunction involves NO-mediated JAK-STAT signalling in rats[J]. Food Chem, 2012, 134(4): 2184
[23]AO GZ, ZHOU MZ, LI YY, et al. Discovery of novel curcumin derivatives targeting xanthine oxidase and urate transporter 1 as anti-hyperuricemic agents[J]. Bioorg Med Chem, 2017, 25(1): 166

相似文献/References:

[1]张建明)△,姚丽娜),李红),等.郑州地区健康体检人群高尿酸血症发病情况调查[J].郑州大学学报(医学版),2009,(01):174.
 ZHANG Jianming),YAO Lina),LI Hong),et al.Study on hyperuricemia in the physical examination population in Zhengzhou area[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(03):174.
[2]姚芳芳),张锐),傅瑞娟),等. 槲皮素对高尿酸血症大鼠黄嘌呤氧化酶和腺苷脱氨酶活性的影响[J].郑州大学学报(医学版),2011,(02):248.
 YAO Fangfang,ZHANG Rui,FU Ruijuan,et al. Effects of quercetin on activity of XOD and ADA in hyperuricemic rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(03):248.
[3]刘琳琳),赵景志),庞超),等.郑州市公务员人群高尿酸血症患病率及影响因素[J].郑州大学学报(医学版),2012,(03):393.
 LIU Linlin),ZHAO Jingzhi),PANG Chao),et al.Prevalence of hyperuricemia and influencing factors among civil servants in Zhengzhou[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(03):393.
[4]杨 璐),王 冠),黎 婷)#,等.三七总皂苷在兔牙齿移动过程中对牙周组织转化生长因子-β1表达的影响*[J].郑州大学学报(医学版),2017,(04):452.[doi:10.13705/j.issn.1671-6825.2017.04.019]
 YANG Lu),WANG Guan),LI Ting),et al.Influence of panax notoginseng saponins on expression of TGF-β1 in periodontal tissue of rabbits during teeth movement[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(03):452.[doi:10.13705/j.issn.1671-6825.2017.04.019]
[5]蒋 明,王昌明#,韩旭惠,等.COPD大鼠原代肺动脉平滑肌细胞中MCP-1、TGF-β1和TLR4蛋白的检测*[J].郑州大学学报(医学版),2017,(05):641.[doi:10.13705/j.issn.1671-6825.2017.05.031]
 JIANG Ming,WANG Changming,HAN Xuhui,et al.Detection of MCP-1,TGF-β1 and TLR4 protein in primary pulmonary artery smooth muscle cells of COPD rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(03):641.[doi:10.13705/j.issn.1671-6825.2017.05.031]

备注/Memo

备注/Memo:
【基金项目】河南省教育厅基础与前沿项目(17A320055)
【作者简介】赵瑛瑛,通信作者,1973年1月生,硕士,主任医师,研究方向:肾小球疾病,E-mail:zhaoyingying9601@163.com
更新日期/Last Update: 2018-05-20