[1]晋 瑞),李 嫚),雷 喆),等.转化生长因子-β1对MC3T3-E1小鼠成骨细胞骨形成的影响[J].郑州大学学报(医学版),2018,(03):378-381.[doi:10.13705/j.issn.1671-6825.2017.10.041]
 JIN Rui),LI Man),LEI Zhe),et al.Effect of transformation growth factor-β1 on osteoblasts and osteogenesis of MC3T3-E1 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(03):378-381.[doi:10.13705/j.issn.1671-6825.2017.10.041]
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转化生长因子-β1对MC3T3-E1小鼠成骨细胞骨形成的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年03期
页码:
378-381
栏目:
应用研究
出版日期:
2018-03-20

文章信息/Info

Title:
Effect of transformation growth factor-β1 on osteoblasts and osteogenesis of MC3T3-E1 cells
作者:
晋 瑞1)李 嫚2)雷 喆1)金迎迎3)
1)西安交通大学第二附属医院医学影像科 西安 710004;2)西安市第三医院科教科 西安 710018;3)西安交通大学第二附属医院肿瘤科 西安 710004
Author(s):
JIN Rui1) LI Man2) LEI Zhe1) JIN Yingying3)
1)Department of Medical Imaging, the Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004;2)Division of Education and Research, the Third Hospital of Xi'an City, Xi'an 710018;3)Department of Oncology, the Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004
关键词:
转化生长因子-β1 Smad2/3 硬骨素 成骨细胞骨形成
Keywords:
TGF-β1 Smad2/3 sclerostin osteoblast formation
分类号:
R336
DOI:
10.13705/j.issn.1671-6825.2017.10.041
摘要:
目的:探讨转化生长因子-β1(TGF-β1)在成骨细胞骨形成中的负向调节作用及其可能作用机制。方法:离体培养MC3T3-E1小鼠成骨细胞系,以10 μg/L的甲状旁腺激素(PTH)或TGF-β1处理细胞6、12、24 h后,应用荧光定量PCR检测硬骨素(SOST)和TGF-β受体ALK5 mRNA表达变化; 取对数生长期的MC3T3-E1细胞进行ALK5-siRNA转染,荧光定量PCR及Western blot法检测ALK5-siRNA转染对TGF-β1作用下MC3T3-E1细胞中SOST mRNA和Smad2/3蛋白表达的影响。结果:在10 μg/L浓度的TGF-β1诱导下,MC3T3-E1细胞中SOST和ALK5 mRNA表达水平均较对照组升高(P<0.05),且表达水平均随着处理时间的延长而上调(P<0.05)。沉默ALK5可部分抑制TGF-β1诱导的SOST mRNA和Smad2/3蛋白表达上调(P<0.05)。结论:TGF-β1可通过激活Smad2/3信号通路上调SOST表达,从而抑制成骨细胞骨形成。
Abstract:
Aim: To investigate the negative regulation of transforming growth factor-β1(TGF-β1)upon osteoblast formation and the mechanism.Methods: The mice osteoblast cell line MC3T3-E1 were cultured in vitro and stimulated with 10 μg/L parathyroid hormone(PTH)or TGF-β1 for 6, 12, 24 h. The expression of sclerostin(SOST)and TGF-β receptor ALK5 mRNA was detected by qRT-PCR. The effect of ALK5-siRNA transfection on SOST mRNA and Smad2/3 protein expression of the MC3T3-E1 cells were detected by qRT-PCR and Western blot, respectively.Results: The expression of SOST mRNA and ALK5 mRNA were significantly up-regulated after 10 μg/L TGF-β1 treatment(P<0.05); and the expression levels were increased with the culture time(P<0.05). The expression of SOST mRNA was significantly decreased while the protein level of Smad2/3 was increased after ALK5 knockdown(P<0.05).Conclusion: TGF-β1 up-regulates the expression of SOST via activating Smad2/3 signaling pathway, thus inhibits osteoblast formation.

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备注/Memo

备注/Memo:
【作者简介】晋瑞,通信作者,女,1978年3月生,硕士,主治医师,研究方向:骨关节疾病,E-mail:racel_327@sina.com
更新日期/Last Update: 2018-05-20