[1]许雅娟,洪 腾,翟闪闪,等.孕期针对性补充叶酸对血清HCY水平的影响[J].郑州大学学报(医学版),2018,(03):381-385.[doi:10.13705/j.issn.1671-6825.2017.11.053]
 XU Yajuan,HONG Teng,ZHAI Shanshan,et al.Effect of targeted supplementation of folic acid during pregnancy on serum homocysteine levels of women[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(03):381-385.[doi:10.13705/j.issn.1671-6825.2017.11.053]
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孕期针对性补充叶酸对血清HCY水平的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年03期
页码:
381-385
栏目:
应用研究
出版日期:
2018-03-20

文章信息/Info

Title:
Effect of targeted supplementation of folic acid during pregnancy on serum homocysteine levels of women
作者:
许雅娟洪 腾翟闪闪柳 蕊任利单余艳茹班彦杰冉利敏胡璐璐罗晓华刘月华韩 宁
郑州大学第三附属医院产科 郑州 450052
Author(s):
XU YajuanHONG TengZHAI ShanshanLIU RuiREN LidanYU YanruBAN YanjieRAN LiminHU LuluLUO XiaohuaLIU YuehuaHAN Ning
Department of Obstetrics, the Third Affiliated Hospital,Zhengzhou University, Zhengzhou 450052
关键词:
妊娠妇女 叶酸利用能力 血清HCY
Keywords:
pregnant women folic acid utilization ability serum homocysteine
分类号:
R714
DOI:
10.13705/j.issn.1671-6825.2017.11.053
摘要:
目的:探讨不同遗传体征的孕妇口服不同剂量的叶酸对血清同型半胱氨酸(homocysteine,HCY)水平的影响。方法:选取定期围产保健的早孕期孕妇1 432人,采集口腔黏膜上皮细胞,用Taqman-MGB探针检测5,10-亚甲基四氢叶酸还原酶(MTHFR)C677T、A1298C和甲蛋氨酸合成还原酶(MTRR)A66G三个位点,判断叶酸利用能力。根据叶酸利用能力分为未发现风险组、低度风险组、中度风险组、高度风险组,其中未发现风险组根据叶酸补充剂量分为0.4 mg叶酸组和未干预组,低、中、高度风险组根据叶酸补充剂量分为:0.4 mg叶酸组和0.8 mg叶酸组,检测早、中、晚孕期研究对象外周血血清HCY水平。结果:未发现风险组中服用0.4 mg叶酸的孕妇血清HCY在早、中、晚孕期均低于未干预孕妇(P<0.05)。低度风险组中,服用0.4 mg叶酸和服用0.8 mg叶酸的孕妇早、中、晚孕期血清HCY水平差异无统计学意义(P>0.05)。中、高风险组中,服用0.4 mg叶酸的孕妇早、中、晚孕期血清HCY均高于服用0.8 mg叶酸的孕妇(P<0.05)。未发现风险组和低风险组补充0.4 mg叶酸和中、高风险组补充0.8 mg叶酸的孕妇血清HCY在孕期均呈降低趋势。结论:不同遗传体征孕妇针对性补充叶酸可使孕期血清HCY水平相对稳定,预防妊娠后期HCY浓度的升高,进而改善妊娠结局。
Abstract:
Aim: To investigate the effects of different doses of folic acid supplementation on the serum homocysteine levels of pregnant women with different genetic characteristics.Methods: A total of 1 432 pregnant women who received regular perinatal care were selected. Oral mucosal epithelial cells were collected from these women. The Taqman-MGB probe was used to examine the C677T and A1298C loci in the 5,10-methylenetetrahydrofolate reductase(MTHFR)gene and the A66G locus in the methionine synthase reductase(MTRR)gene. Based on their folic acid utilization ability, the included women were allocated into no-risk, low-risk, moderate-risk, and high-risk groups. Based on the dosage of folic acid supplementation, the no-risk group was further divided into non-intervention group and 0.4 folic acid mg group, and the low-risk, moderate-risk, and high-risk groups were further divided into 0.4 mg and 0.8 mg folic acid groups. The level of homocysteine in the peripheral blood of the study subjects during early, mid-, and late pregnancy was examined.Results: In no-risk group, the pregnant women with 0.4 mg folic acid supplementation had lower serum level of homocysteine during early, mid-, and late pregnancy than those not intervented(P<0.05); in low-risk group, there was no significant difference in serum homocysteine level between the pregnant women with 0.4 mg folic acid supplementation and 0.8 mg folic acid supplementation during early, mid-, and late pregnancy(P>0.05); in moderate-risk group and high-risk group, during early, mid-, and late pregnancy, the serum homocysteine level in the pregnant women with 0.4 mg folic acid supplementation was significantly higher compared with those with 0.8 mg folic acid supplementation(P<0.05). The serum homocysteine level of the pregnant women with 0.4 mg folic acid supplementation in no-risk group and low-risk group as well as those with 0.8 mg folic acid supplementation in moderate-risk group and high-risk group showed a downward trend during pregnancy.Conclusion: Individuals with distinct genetic characteristics have different demands for folic acid, so the personalized supplementation of folic acid could keep a stable level of serum homocysteine in pregnancy, which could prevent the increase of late pregnancy homocysteine concentration, and improve pregnancy outcome.

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备注/Memo

备注/Memo:
【基金项目】河南省医学科技攻关计划基金资助项目(201403110)
【作者简介】许雅娟,通信作者,女,1963年4月生,本科,主任医师,研究方向:围产医学,E-mail:cnzzzsl@163.com
更新日期/Last Update: 2018-05-20