[1]韩笑笑,刘丽雅,谭超月,等.稳定表达荧光素酶基因的TC-1细胞系的构建[J].郑州大学学报(医学版),2018,(04):429-432.[doi:10.13705/j.issn.1671-6825.2017.10.005]
 HAN Xiaoxiao,LIU Liya,TAN Chaoyue,et al.Establishment of luciferase-labeled TC-1 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(04):429-432.[doi:10.13705/j.issn.1671-6825.2017.10.005]
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稳定表达荧光素酶基因的TC-1细胞系的构建()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年04期
页码:
429-432
栏目:
论著
出版日期:
2018-07-20

文章信息/Info

Title:
Establishment of luciferase-labeled TC-1 cells
作者:
韩笑笑刘丽雅谭超月李 元高君碧韩丽萍
郑州大学第一附属医院妇科; 河南省高等学校临床医学重点学科开放实验室 郑州 450052
Author(s):
HAN XiaoxiaoLIU LiyaTAN ChaoyueLI YuanGAO Junbi HAN Liping
Department of Gynecology, the First Affiliated Hospital,Zhengzhou University; Key-Disciplines Laboratory of Clinical-Medicine of Henan Province,Zhengzhou 450052
关键词:
TC-1细胞 荧光素酶 活体成像 生物发光 宫颈癌
Keywords:
TC-1 cell luciferase imaging in vivo bioluminescence cervical cancer
分类号:
R711.74
DOI:
10.13705/j.issn.1671-6825.2017.10.005
摘要:
目的:通过表达荧光素酶的慢病毒载体感染TC-1细胞株,建立稳定表达荧光素酶的TC-1细胞株。方法:根据荧光素酶基因设计引物,PCR扩增获得目的基因,构建表达荧光素酶的慢病毒载体并包装重组慢病毒,感染TC-1细胞。嘌呤霉素加压筛选,鉴定。用双荧光素酶报告基因检测试剂盒及体外成像系统进行荧光素酶活性检测。结果:经检测证实细胞感染及筛选后,成功构建了稳定高表达荧光素酶的TC-1细胞。结论:获得荧光素酶稳定表达的TC-1细胞,转染效果确切,荧光素酶基因表达稳定。
Abstract:
Aim: To establish luciferase-labeled TC-1 cells.Methods: TC-1 cells were infected by lentivirus packaged by recombinant lentiviral vectors containing luciferase gene. Puromycin was used to filter the cells. The expressing of luciferase in TC-1-luc cells was detected by immunofluorescence imaging and in vivo imaging system.Results: After cell infection and screening, it was shown that a stable TC-1 cell line stably expressing luciferase had been built.Conclusion: TC-1 cell line stably expressing luciferase has been obtained.

参考文献/References:

[1] BOSCH FX,MANOS MM,MU(~overN)OZ N,et al.Prevalence of human papillomavirus in cervical cancer: a worldwide perspective. International biological study on cervical cancer(IBSCC)Study Group[J].J Natl Cancer Inst,1995,87(11):796
[2] WANG YY,LI L,WEI S,et al.Human Papillomavirus(HPV)infection in women participating in cervical cancer screening from 2006 to 2010 in Shenzhen City, South China[J].Asian Pac J Cancer Prev,2013,14(12):7483
[3] SEKIGUCHI Y,OWADA J,OISHI H,et al.Noninvasive monitoring of β-cell mass and fetal β-cell genesis in mice using bioluminescence imaging[J].Exp Anim,2012,61(4):445
[4] JENKINS DE,HORNIG YS,OEI Y,et al.Bioluminescent human breast cancer cell lines that permit rapid and sensitive in vivo detection of mammary tumors and multiple metastases in immune deficient mice[J].Breast Cancer Res,2005,7(4):R444
[5] LIN KY,GUARNIERI FG,STAVELEY-O'CARROLL KF,et al.Treatment of established tumors with a novel vaccine that enhances major histocompatibility class Ⅱ presentation of tumor antigen[J].Cancer Res,1996,56(1):21
[6] 陈惠,李坤雨,陈斐,等.用于活体成像的小鼠肝癌移植瘤模型的建立[J].兰州大学学报(医学版),2012,38(1):10
[7] GOMES CM,WELLING M,QUE I,et al.Functional imaging of multidrug resistance in an orthotopic model of osteosarcoma using 99mTc-sestamibi[J].Eur J Nucl Med Mol Imaging,2007,34(11):1793
[8] DEROOSE CM,DE A,LOENING AM,et al.Multimodality imaging of tumor xenografts and metastases in mice with combined small-animal PET, small-animal CT, and bioluminescence imaging[J].J Nucl Med,2007,48(2):295
[9] RODA A.Discovery and development of the green fluorescent protein,GFP:the 2008 Nobel Prize[J].Anal Bioanal Chem,2010,396(5):1619
[10]YU YA,TIMIRYASOVA T,ZHANG Q,et al.Optical imaging: bacteria, viruses, and mammalian cells encoding light-emitting proteins reveal the locations of primary tumors and metastases in animals[J].Anal Bioanal Chem,2003,377(6):964
[11]KATZ MH,TAKIMOTO S,SPIVACK D,et al.An imageable highly metastatic orthotopic red fluorescent protein model of pancreatic cancer[J].Clin Exp Metastasis,2004,21(1):7
[12]董苑,李彩霞,赵娴,等.荧光素酶基因稳定表达的间充质干细胞系的构建[J].中华显微外科杂志,2014(6):569
[13]WINNARD PT JR,KLUTH JB,RAMAN V.Noninvasive optical tracking of red fluorescent protein-expressing cancer cells in a model of metastatic breast cancer[J].Neoplasia,2006,8(10):796
[14]DUFORT S,SANCEY L,WENK C,et al.Optical small animal imaging in the drug discovery process[J].Biochim Biophys Acta,2010,1798(12):2266
[15]苏云术,徐利军,周鸿敏,等.活体成像技术监测肿瘤生长动态的研究[J].医学研究生学报,2014,27(2):143
[16]SUN YY,PENG S,HAN L,et al.Local HPV recombinant vaccinia boost following priming with an HPV DNA vaccine enhances local HPV-specific CD8+T-cell-mediated tumor control in the genital tract[J].Clin Cancer Res,2016,22(3):657

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备注/Memo

备注/Memo:
【基金项目】国家自然科学基金资助项目(U1604172)
【作者简介】韩丽萍,通信作者,女,1969年8月生,博士,教授,研究方向:妇科肿瘤与内分泌,E-mail:hanliping0825@163.com
更新日期/Last Update: 2018-06-20