[1]孙庆敏,王 进.miRGAAB2200a与金雀异黄酮对胃癌细胞生长及侵袭能力的影响[J].郑州大学学报(医学版),2018,(04):474-477.[doi:10.13705/j.issn.1671-6825.2017.11.042]
 SUN Qingmin,WANG Jin.Synergistic effect of miRGAAB2200a and genistein on growth and invasion of gastric cancer cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(04):474-477.[doi:10.13705/j.issn.1671-6825.2017.11.042]
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miRGAAB2200a与金雀异黄酮对胃癌细胞生长及侵袭能力的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年04期
页码:
474-477
栏目:
应用研究
出版日期:
2018-07-20

文章信息/Info

Title:
Synergistic effect of miRGAAB2200a and genistein on growth and invasion of gastric cancer cells
作者:
孙庆敏王 进
南京中医药大学附属医院药学部 南京 210029
Author(s):
SUN QingminWANG Jin
Department of Pharmacy,the Affiliated Hospital,Nanjing University of Chinese Medicine,Nanjing 210029
关键词:
miR GAAB2 200a 金雀异黄酮 EphA2 胃癌
Keywords:
miR GAAB2 200a genistein EphA2 gastric cancer
分类号:
R735.2
DOI:
10.13705/j.issn.1671-6825.2017.11.042
摘要:
目的:探讨miRGAAB2200a与金雀异黄酮对3种胃癌细胞生长及侵袭能力的影响及可能机制。方法:MGC803、BGC823、MKN45细胞分别分为4组:阴性对照组(转染阴性对照NC mimic)、金雀异黄酮组(加入50 μmol/L的金雀异黄酮)、miRGAAB2200a组(转染miRGAAB2200a mimic)、联合组(miRGAAB2200a mimic转染联合50 μmol/L的金雀异黄酮处理),MTT法观察处理24 h对细胞生长的影响,Transwell小室实验观察细胞侵袭能力的变化,RTGAAB2PCR及Western blot检测各组细胞EphA2 mRNA及蛋白的表达。结果:miRGAAB2200a与金雀异黄酮单用均可抑制部分胃癌细胞的生长、侵袭及EphA2蛋白的表达,两者交互作用仅出现在BGC823细胞中(P<0.05)。结论:miRGAAB2200a与金雀异黄酮可能通过调控EphA2抑制胃癌细胞生长及侵袭,且二者存在部分协同作用。
Abstract:
Aim: To investigate the effects and possible mechanisms of miRGAAB2200a and genistein on the growth and invasion of gastric cancer cells.Methods: The human gastric cancer cell lines MGC803, BGC823 and MKN45 were used as research objects. The cells were divided into 4 groups,negative control group(transfected with NC mimic),genistein group(treated by 50 μmol/L genistein),miRGAAB2200a group(transfected with miRGAAB2200a mimic),and combination group(transfected with miRGAAB2200a mimic and treated by 50 μmol/L genistein). The effects of miRGAAB2200a and genistein on the growth of gastric cancer cells were observed by MTT assay.Transwell chamber was used to verify the effect on cell invasion. RT-PCR and Western blot were used to observe the effects of miRGAAB2200a and genistein on EphA2 mRNA and protein.Results: Both miRGAAB2200a and genistein could inhibit the growth and invasion of part of the gastric cancer cells and inhibit the expression of EphA2 protein, and the synergistic effects of miR-200a and genistein were only found in BGC823 cells(P<0.05).Conclusion: miRGAAB2200a and genistein might inhibit the growth and invasion of gastric cancer cells through down-regulation of EphA2,and there are some synergistic effects.

参考文献/References:

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备注/Memo

备注/Memo:
【基金项目】江苏省青年医学人才项目(QNRC2016641); 南京市科技计划项目(201201055); 江苏省中医院培育项目(Y16002); 江苏省中医院高峰学术人才项目(Y2018RC33)
【作者简介】王进,通信作者,女,1966年4月生,本科,副主任药师,研究方向:肿瘤药理学,EGAAB2mail:sunqingminnjmu@163.com
更新日期/Last Update: 2018-06-20