[1]李德江),王 兵),马立人).沉默Galectin-3基因对甲状腺癌细胞生长的影响[J].郑州大学学报(医学版),2018,(04):488-492.[doi:10.13705/j.issn.1671-6825.2018.02.004]
 LI Dejiang),WANG Bing),MA Liren).Effects of silencing Galectin-3 gene on the growth of thyroid cancer cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(04):488-492.[doi:10.13705/j.issn.1671-6825.2018.02.004]
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沉默Galectin-3基因对甲状腺癌细胞生长的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年04期
页码:
488-492
栏目:
应用研究
出版日期:
2018-07-20

文章信息/Info

Title:
Effects of silencing Galectin-3 gene on the growth of thyroid cancer cells
作者:
李德江1)王 兵2)马立人3)
1)平顶山市中医医院外科 河南平顶山 467000;2)郑州大学第五附属医院血管外科 郑州 450052;3)平顶山市中医医院周围血管科 河南平顶山 467000
Author(s):
LI Dejiang1)WANG Bing2)MA Liren3)
1)Department of Surgery, Pingdingshan Hospital of Traditional Chinese Medicine, Pingdingshan, Henan 467000;2)Department of Vascular Surgery, the Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou 450052;3)Department of Peripheral Vascular, Pingdingshan Hospital of Traditional Chinese Medicine, Pingdingshan, Henan 467000
关键词:
RNA干扰 Galectin-3基因 甲状腺癌 增殖 凋亡
Keywords:
RNA interference Galectin-3 gene thyroid carcinoma proliferation apoptosis
分类号:
R736.1
DOI:
10.13705/j.issn.1671-6825.2018.02.004
摘要:
目的:探讨沉默半乳凝集素-3(Galectin-3)基因表达对甲状腺癌细胞生长的影响。方法:采用RT-PCR及Western blot方法检测46例甲状腺癌及其相应的距癌灶1 cm以上癌旁正常组织中Galectin-3 mRNA和蛋白的表达。将阴性对照(NC)-siRNA、Galectin-3-siRNA转染人甲状腺癌SW579细胞,转染12、24、48 h后CCK-8法检测细胞增殖率; 转染48 h后,RT-PCR及Western blot检测Galectin-3 mRNA及蛋白的表达,流式细胞术检测凋亡率,Western blot法检测Ki67、PCNA、Cleaved caspase3、PI3K、AKT、p-AKT蛋白。以未转染细胞作空白对照。结果:甲状腺癌组织中Galectin-3 mRNA及蛋白的表达均高于癌旁正常组织(P<0.01)。与空白对照和NC-siRNA组比较,Galectin-3-siRNA组细胞Galectin-3 mRNA及蛋白表达降低(P<0.01); 细胞增殖率降低,凋亡率升高(P<0.01); Ki67、PI3K、PCNA和p-AKT蛋白表达降低,Cleaved caspase3蛋白表达升高(P<0.01)。结论:Galectin-3可能通过PI3K/AKT信号通路调控甲状腺癌细胞的生长。
Abstract:
Aim: To investigate the effect of silencing Galectin-3 gene on the growth of thyroid carcinoma cells.Methods: RT-PCR and Western blot methods were used to detect Galectin-3 mRNA and protein in 46 cases of thyroid carcinoma tissue and the corresponding normal tissue. Negative control(NC)-siRNA and Galectin-3-siRNA were transfected into human thyroid carcinoma SW579 cells after 12,24,48 h treatment, cell proliferation rate was detected by CCK-8 test; after 48 h, the Galectin-3 mRNA and protein were detected by RT-PCR and Western blot,respectively, apoptosis was detected by flow cytometry, and Ki67,PCNA, Cleaved caspase3, PI3K,AKT, and p-AKT proteins were detected by Western blot. The cells without transfection were the blank control.Results: The expressions of Galectin-3 mRNA and protein in thyroid carcinoma tissue were significantly higher than those in the corresponding normal tissue(P<0.01). Compared with those of the blank control and NC-siRNA group, the expression of Galectin-3 mRNA and protein in Galectin-3-siRNA group were significantly lower(P<0.01); the cell proliferation rate decreased and cell apoptosis rate increased(P<0.01); the expressions of Ki67, PCNA, PI3K, p-AKT protein decreased, and the expressions of Cleaved caspase3 protein increased(P<0.01).Conclusion: Galectin-3 may regulate the growth of thyroid carcinoma cells through PI3K/AKT signaling pathway.

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备注/Memo

备注/Memo:
【作者简介】马立人,通信作者,男,1963年4月生,本科,主任医师,研究方向:血管外科,E-mail:13781880999@163.com
更新日期/Last Update: 2018-06-20