[1]李嘉蔚),胡雪菲),韩 鹏),等.斑马鱼体内氧化损伤标志物筛选[J].郑州大学学报(医学版),2018,(04):499-502.[doi:10.13705/j.issn.1671-6825.2018.04.109]
 LI Jiawei),HU Xuefei),HAN Peng),et al.Screening for markers of oxidative damage in zebrafish[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(04):499-502.[doi:10.13705/j.issn.1671-6825.2018.04.109]
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斑马鱼体内氧化损伤标志物筛选()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年04期
页码:
499-502
栏目:
应用研究
出版日期:
2018-07-20

文章信息/Info

Title:
Screening for markers of oxidative damage in zebrafish
作者:
李嘉蔚123)胡雪菲123)韩 鹏123)陈呢喃1)陆一鸣123)
1)上海交通大学医学院附属瑞金医院中法生命科学和基因组研究中心 上海 200025;2)上海交通大学医学院附属瑞金医院北院急诊科 上海 200025;3)上海交通大学医学院附属瑞金医院急诊科 上海 200025
Author(s):
LI Jiawei123) HU Xuefei123)HAN Peng123) CHEN Ninan1) LU Yiming123)
1)Sino-French Research Center for Life Sciences and Genomics, Ruijin Hospital,School of Medicine,Shanghai Jiaotong University, Shanghai 200025;2)Department of Emergency, Ruijin Hospital North Branch, School of Medicine,Shanghai Jiaotong University, Shanghai 200025;3)Department of Emergency, Ruijin Hospital, School of Medicine,Shanghai Jiaotong University, Shanghai 200025, China
关键词:
斑马鱼 氧化应激 端粒 端粒重复序列结合因子2
Keywords:
zebrafish oxidative stress telomere telomere repeat binding factor 2
分类号:
R33
DOI:
10.13705/j.issn.1671-6825.2018.04.109
摘要:
目的:探讨以端粒为靶点的氧化损伤标志物的筛选,进而可能可以用来临床上早期发现或预报氧化损伤。方法:采用端粒重复序列结合因子2(TRF2)基因敲除(TRF2-/-)斑马鱼胚胎作为氧化损伤模型,RT-qPCR法检测野生型和TRF2-/-斑马鱼胚胎中TRF2、PUM3、PACSIN3 mRNA的表达情况。构建pCS2-PUM3、pCS2-PACSIN3质粒并体外转录后,将mRNA分别显微注射至TRF2-/-斑马鱼受精卵中,比较注射组与未注射组斑马鱼畸形率。结果:RT-qPCR结果表明,与野生型斑马鱼胚胎相比,TRF2-/-斑马鱼胚胎TRF2、PUM3、PACSIN3 mRNA的表达量下降(P<0.05)。显微注射PUM3 mRNA至TRF2-/-斑马鱼受精卵后斑马鱼胚胎畸形率下降(P<0.05)。结论:TRF2能影响氧化应激相关基因PUM3、PACSIN3的表达,且PUM3能补救TRF2缺失造成的氧化损伤相关器官畸形。
Abstract:
Aim: To screen oxidative damage markers targeting telomeres, which may then be used to clinically detect or predict oxidative damage early.Methods: The telomeric repeat binding factor 2(TRF2)knockout(TRF2-/-)zebrafish embryos were used as an oxidative damage model. RT-qPCR was used to detect the expression of TRF2, PUM3 and PACSIN3 mRNA in wild-type and TRF2-/-zebrafish embryos. After pCS2-PUM3 and pCS2-PACSIN3 plasmids were constructed and in vitro transcribed, their mRNA were into TRF2-/-zebrafish fertilized eggs, and the zebrafish malformation rates in the groups with injection and without injection were summarized and statistically analyzed.Results: RT-qPCR results showed that the TRF2, PUM3, and PACSIN3 mRNA expression levels in TRF2-/-zebrafish embryos were significantly lower than those in wild-type zebrafish embryos(P<0.05).After microinjection of zebrafish PUM3 mRNA into TRF2-/-zebrafish fertilized eggs, the malformation rates of zebrafish embryos decreased(P<0.05).Conclusion: TRF2 can affect the expressions of oxidative stress related genes PUM3 and PACSIN3, and PUM3 can remedy the oxidative damage-related organ malformation caused by TRF2 deficiency. Targeting these molecules is expected to detect or predict oxidative stress early in clinical field.

参考文献/References:

[1] HOFFMAN WH, SIEDLAK SL, WANG Y, et al. Oxidative damage is present in the fatal brain edema of diabetic ketoacidosis[J]. Brain Res, 2011, 1369: 194
[2] ZHAO G, CAO K, XU C, et al. Crosstalk between mitochondrial fission and oxidative stress in paraquat-induced apoptosis in mouse alveolar type Ⅱ cells[J]. Int J Biol Sci,2017,13(7): 888
[3] 张锡刚, 汤雪萍, 李光, 等. 早期大剂量甲泼尼龙联合环孢素A冲击治疗对口服百草枯中毒患者预后的影响[J]. 解放军医学杂志, 2007, 32(12): 1296
[4] COLUZZI E, COLAMARTINO M, COZZI R, et al. Oxidative stress induces persistent telomeric DNA damage responsible for nuclear morphology change in mammalian cells[J]. PLoS One, 2014, 9(10): e110963
[5] LUO Z, FENG X, WANG H, et al. Mir-23a induces telomere dysfunction and cellular senescence by inhibiting TRF2 expression[J]. Aging Cell, 2015, 14(3): 391
[6] RICHTER T, PROCTOR C. The role of intracellular peroxide levels on the development and maintenance of telomere-dependent senescence[J]. Exp Gerontol, 2007, 42(11): 1043
[7] LIVAK KJ, SCHMITTGEN TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 2001, 25(4): 402
[8] 曾俊,包可久,陈刘,等.糖尿病酮症酸中毒患者血pH值、血糖水平与心肌酶、淀粉酶的相关性分析[J].疑难病杂志,2018,17(5):468
[9] 张清贵, 翁孝刚, 王卫民, 等. 老年糖尿病酮症酸中毒26例[J]. 郑州大学学报(医学版), 2003, 38(1): 132
[10]李云婷, 陈丽荣, 李诗阳, 等. 老年糖尿病酮症酸中毒患者外周血氧化应激及炎症指标的改变[J]. 中国老年学杂志, 2017, 37(13): 3205
[11]韩东明, 王清华, 孙建明, 等. 百草枯中毒14例肺部X线与CT表现[J]. 郑州大学学报(医学版), 2004, 39(1): 162
[12]GALANO JM, LEE YY, DURAND T, et al.The use of isoprostanoids as biomarkers of oxidative damage, and their role in human dietary intervention studies[J]. Free Radic Res, 2015, 49(5): 583
[13]IBITOYE R, KEMP K, RICE C, et al. Oxidative stress-related biomarkers in multiple sclerosis: a review[J]. Biomark Med, 2016, 10(8): 889
[14]YANG D, XIONG Y, KIM H, et al. Human telomeric proteins occupy selective interstitial sites[J]. Cell Res, 2011, 21(7): 1013
[15]SIMONET T, ZARAGOSI LE, PHILIPPE C, et al. The human TTAGGG repeat factors 1 and 2 bind to a subset of interstitial telomeric sequences and satellite repeats[J]. Cell Res, 2011, 21(7): 1028

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备注/Memo

备注/Memo:
【基金项目】国家自然科学基金项目(81171846,81671900)
【作者简介】陆一鸣,通信作者,男,1963年11月生,博士,教授,主任医师,研究方向:急诊及重症医学、创伤救治、临床毒理学、氧化应激、端粒与衰老,E-mail:Luyiming@rjh.com.cn
更新日期/Last Update: 2018-06-20