[1]张成刚.CPEB4对脑胶质瘤细胞增殖、迁移的影响[J].郑州大学学报(医学版),2018,(05):599-603.[doi:10.13705/j.issn.1671-6825.2018.01.064]
 ZHANG Chenggang.Effect of CPEB4 on proliferation and migration of human glioma cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(05):599-603.[doi:10.13705/j.issn.1671-6825.2018.01.064]
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CPEB4对脑胶质瘤细胞增殖、迁移的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年05期
页码:
599-603
栏目:
应用研究
出版日期:
2018-09-20

文章信息/Info

Title:
Effect of CPEB4 on proliferation and migration of human glioma cells
作者:
张成刚
济南市第三人民医院神经外科 济南 250132
Author(s):
ZHANG Chenggang
Department of Neurosurgery, Jinan Third People's Hospital, Jinan 250132
关键词:
脑胶质瘤 CPEB4 增殖 迁移 JAK/STAT3信号通路
Keywords:
glioma CPEB4 proliferation migration JAK/STAT3 signal pathway
分类号:
R739.41
DOI:
10.13705/j.issn.1671-6825.2018.01.064
摘要:
目的:探讨胞质多聚腺苷酸化元件结合蛋白4(CPEB4)对脑胶质瘤细胞增殖、迁移的影响。方法:采用实时荧光定量PCR法检测40例脑胶质瘤组织及对应的癌旁正常组织中CPEB4 mRNA的表达水平。分别将CPEB4 siRNA及无义siRNA转染至脑胶质瘤细胞U87中,Western blot法检测细胞中CPEB4、MMP-9、MMP-2、STAT3、p-STAT3、JAK2和p-JAK2蛋白的表达水平,MTT法检测细胞存活率,细胞划痕实验检测细胞迁移距离,以未处理细胞作对照。使用50 μmol/L特异性JAK2抑制剂AG490作用于转染CPEB4 siRNA的U87细胞48 h后,检测细胞存活率及迁移距离,以未处理细胞和AG490处理的U87细胞作对照。结果:脑胶质瘤组织中CPEB4 mRNA表达水平高于癌旁正常组织(P<0.05)。与对照组及无义siRNA组相比,CPEB4 siRNA组细胞CPEB4蛋白表达水平下降(P<0.05),细胞存活率、迁移距离降低(P<0.05),MMP-9、MMP-2、STAT3、p-STAT3、JAK2和p-JAK2蛋白表达水平下降(P<0.05)。与对照组相比,AG490组与AG490+CPEB4 siRNA组细胞存活率和迁移距离均降低(P<0.05); 与AG490组相比,AG490+CPEB4 siRNA组细胞存活率与迁移距离降低(P<0.05)。结论:CPEB4在脑胶质瘤组织中高表达,可能通过上调JAK/STAT3信号通路促进脑胶质瘤细胞的增殖与迁移。
Abstract:
Aim:To investigate the effect of CPEB4 on the proliferation and migration of human glioma cells.Methods:The level of CPEB4 mRNA in 40 cases of glioma cancer and paracancerous normal tissue was detected by qRT-PCR. CPEB4 siRNA and nonsense siRNA were transfected into U87 cells for 48 h, the expressions of CPEB4, MMP-9, MMP-2, STAT3, p-STAT3, JAK2 and p-JAK2 were detected by Western blot, cell proliferation was measured by MTT assay, cell scratch test was used to measure the cell migration distance,the proliferation and migration distance of U87 cells transfected with CPEB4 siRNA after 50 μmol/L AG490 treatment for 48 h were detected.The cells without any treatment were the control.Results:The expression of CPEB4 mRNA in glioma tissue was significantly higher than that in paracancerous normal tissue(P<0.05). Compared with the two control groups, the expression of CPEB4 protein in CPEB4 siRNA group decreased(P<0.05), cell survival and migration distance were decreased(P<0.05), and the expressions of MMP-9, MMP-2, STAT3, p-STAT3, JAK2 and p-JAK2 decreased(P<0.05). Compared with the control group, the proliferation and migration distance in AG490 group and AG490+CPEB4 siRNA group were significantly reduced(P<0.05); compared with AG490 group, those in AG490+CPEB4 siRNA group were significantly reduced(P<0.05).Conclusion:CPEB4 is highly expressed in glioma tissue, and might promote the proliferation and migration of glioma cells by up-regulating JAK/STAT3 signal pathway.

参考文献/References:

[1] MAN JH,SHOEMAKE JD,MA TP,et al.Hyperthermia Sensitizes Glioma Stem-like Cells to Radiation by Inhibiting AKT Signaling[J].Cancer Res,2015,75(8):1760
[2] 权俊杰,屈建强,周乐.肝细胞再生磷酸因子-1在脑胶质瘤组织中的表达及对脑胶质瘤细胞凋亡的影响[J].河北医学,2017,23(5):724
[3] LIAO AH,CHOU HY,HSIEH YL,et al.Enhanced therapeutic epidermal growth factor receptor(EGFR)antibody delivery via pulsed ultrasound with targeting microbubbles for glioma treatment[J].J Med Biol Eng,2015,35(2):156
[4] 杨如意,赵普学,许自强,等.人脑胶质瘤组织中HMGB1、TLR4的表达[J].郑州大学学报(医学版),2018,53(2):213
[5] CHEN W,HU Z,LI XZ,et al.CPEB4 interacts with Vimentin and involves in progressive features and poor prognosis of patients with astrocytic tumors[J].Tumour Biol,2016,37(4):5075
[6] HESSMANN E,SCHNEIDER G,ELLENRIEDER V,et al.MYC in pancreatic cancer: novel mechanistic insights and their translation into therapeutic strategies[J].Oncogene,2016,35(13):1609
[7] 刘志军,周海存,陈小兵, 等.人脑胶质瘤组织中CPEB4的表达及与细胞迁移和侵袭的关系[J].郑州大学学报(医学版),2016,51(5):622
[8] 秦荣.脑胶质瘤靶标CPEB4参与增殖侵袭的分子机制及预后相关性研究[D].上海:第二军医大学,2014.
[9] CALDERONE V,GALLEGO J,FERNANDEZ-MIRANDA G,et al.Sequential functions of CPEB1 and CPEB4 regulate pathologic expression of vascular endothelial growth factor and angiogenesis in chronic liver disease[J].Gastroenterology,2016,150(4):982
[10]HU W,YANG Y,XI S,et al.Expression of CPEB4 in human glioma and its correlations with prognosis[J].Medicine(Baltimore),2015,94(27):e979
[11]CIAFRÈ SA,GALARDI S.microRNAs and RNA-binding proteins:a complex network of interactions and reciprocal regulations in cancer[J].RNA Biol,2013,10(6):935
[12]ZHONG X,XIAO Y,CHEN C,et al.MicroRNA-203-mediated posttranscriptional deregulation of CPEB4 contributes to colorectal cancer progression[J].Biochem Biophys Res Commun,2015,466(2):206
[13]TSAI LY,CHANG YW,LEE MC,et al.Biphasic and stage-associated expression of CPEB4 in hepatocellular carcinoma[J].PLoS One,2016,11(5):e0155025
[14]张鹏,纪亮,张翠香,等.基质金属蛋白酶促进瘢痕疙瘩成纤维细胞迁移及其意义[J].中国现代医学杂志,2013,23(3):11
[15]赵明静,高英,王玲玲,等.整合素连接激酶在肺癌细胞中过度表达并通过核因子-κB途径介导基质金属蛋白酶-9上调促进肺癌细胞迁移和侵袭[J].中华结核和呼吸杂志,2014,37(2):98
[16]FLEMING JD,GIRESI PG,LINDAHL-ALLEN M,et al.STAT3 acts through pre-existing nucleosome-depleted regions bound by FOS during an epigenetic switch linking inflammation to cancer[J].Epigenetics Chromatin,2015,8:7
[17]FLYNN R,PAZ K,DU J,et al.Targeted Rho-associated kinase 2 inhibition suppresses murine and human chronic GVHD through a Stat3-dependent mechanism[J].Blood,2016,127(17):2144
[18]LUCHMAN HA,JENSEN KV,AMAN A,et al.Combinatorial strategies for glioblastoma using brain tumor-initiating cells: targeting the JAK/STAT and EGFR pathways[J].Cancer Res,2016,76(14):279
[19]MIKLOSSY G,YOUN UJ,YUE P,et al.Hirsutinolide series inhibit stat3 activity, alter GCN1, MAP1B, Hsp105, G6PD, Vimentin, TrxR1, and importin alpha-2 expression, and induce antitumor effects against human glioma[J].J Med Chem,2015,58(19):7734

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备注/Memo

备注/Memo:
【基金项目】山东省自然科学基金资助项目(ZR2016HL097)
【作者简介】张成刚,男, 1971 年1月生,硕士,副主任医师,研究方向:颅脑创伤及颅内肿瘤,E-mail: rbl5px@163.com
更新日期/Last Update: 2018-09-20