[1]吴丽梦),李 恩),周小翠),等.miR-499-5p干预对心肌梗死大鼠心肌细胞凋亡的影响[J].郑州大学学报(医学版),2018,(05):614-618.[doi:10.13705/j.issn.1671-6825.2017.12.094]
 WU Limeng),LI En),ZHOU Xiaocui),et al.Effects of miR-499-5p intervention on myocardial cell apoptosis in rats with myocardial infarction[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(05):614-618.[doi:10.13705/j.issn.1671-6825.2017.12.094]
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miR-499-5p干预对心肌梗死大鼠心肌细胞凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年05期
页码:
614-618
栏目:
应用研究
出版日期:
2018-09-20

文章信息/Info

Title:
Effects of miR-499-5p intervention on myocardial cell apoptosis in rats with myocardial infarction
作者:
吴丽梦1)李 恩1)周小翠2)郭小宏1)王 蒙1)赵英杰1)汪 涛2)
1)郑州大学第二附属医院心血管内科 郑州 450014;2)郑州大学第二附属医院老年医学科 郑州 450014
Author(s):
WU Limeng1)LI En1) ZHOU Xiaocui2)GUO Xiaohong1)WANG Meng1) ZHAO Yingjie1)WANG Tao2)
1)Department of Cardiovascular Medicine, the Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014;2)Geriatric Department, the Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014
关键词:
miR-499-5p 心肌梗死 凋亡 Sox6 Bax Bcl-xl 大鼠
Keywords:
miR-499-5p myocardial infarction apoptosis Sox6 Bax Bcl-xl rat
分类号:
R541.4
DOI:
10.13705/j.issn.1671-6825.2017.12.094
摘要:
目的:探究miR-499-5p在大鼠心肌梗死后心肌细胞凋亡中的作用和相关机制。方法:80只大鼠随机分为假手术组、心肌梗死组(AMI组)、AMI+miR-499-5p mimics组(干预组)、AMI+scramble-NC组(阴性对照组)。TUNEL法检测心肌细胞凋亡; qRT-PCR检测Sox6 mRNA表达; Western blot分析Sox6、Bax、Bcl-xl蛋白表达; 超声心动图评估心功能; 荧光素酶报告实验检测miR-499-5p是否靶向调控Sox6 3'非编码区。结果:与假手术组比较,AMI组心肌细胞凋亡率增高,Sox6 mRNA和蛋白及Bax蛋白表达增高,Bcl-xl蛋白表达降低,EF值和FS值均降低(P均<0.05); 与阴性对照组、AMI组比较,干预组心肌细胞凋亡率、Sox6 mRNA和蛋白及Bax蛋白表达降低,Bcl-xl蛋白表达增高,EF、FS值增加(P均<0.05)。荧光素酶报告实验提示miR-499-5p 特异性调控Sox6 3'末端非编码序列。结论:miR-499-5p可能通过靶向抑制Sox6的表达来抑制心肌细胞凋亡,最终改善心功能。
Abstract:
Aim: To investigate the role of miR-499-5p in cardiomyocyte apoptosis after myocardial infarction in rats and explore its molecular mechanism.Methods: The rats were randomly allocated into sham operation group, AMI group, intervention group(AMI+intravenous injection of miR-499-5p mimics after pulse ligation),and negative control group(AMI+intravenous injection of negative control sequence).Cardiomyocyte apoptosis was detected by TUNEL staining. Sox6 mRNA was detected by qRT-PCR; the expressions of Sox6, Bax, Bcl-xl protein were detected by Western blot; heart function was detected by Vevo 2100. Luciferase reporter assay was used to detect whether miR-499-5p targeted Sox6 3' non-coding region.Results: Compared with the sham operation group,the apoptosis rate, Sox6 and Bax expression increased,while Bcl-xl expression,the values of EF and FS decreased in AMI group(P<0.05).Compared with the negative control group and AMI group, the apoptosis rate,Sox6,Bax protein decreased,while Bcl-xl expression and the values of EF and FS increased.Luciferase reporter assay showed that miR-499-5p specifically regulated the 3' non-coding region of Sox6.Conclusion:miR-499-5p may inhibit cardiomyocyte apoptosis by targeted inhibition of Sox6 expression, and ultimately improve cardiac function.

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备注/Memo

备注/Memo:
【基金项目】河南省重点科技攻关项目(142102310109,172102310376)
【作者简介】汪涛,通信作者,女,1963年11月生,硕士,教授,研究方向:冠心病基础与临床,E-mail:799931739@qq.com
更新日期/Last Update: 2018-09-20