[1]孙正路),张艳停),徐 刚),等.小鼠结肠炎相关结直肠癌发展过程中大肠组织IL-9的表达[J].郑州大学学报(医学版),2018,(05):622-625.[doi:10.13705/j.issn.1671-6825.2018.03.006]
 SUN Zhenglu),ZHANG Yanting),XU Gang),et al.Dynamic changes of IL-9 in development of colitis associated colorectal cancer in mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(05):622-625.[doi:10.13705/j.issn.1671-6825.2018.03.006]
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小鼠结肠炎相关结直肠癌发展过程中大肠组织IL-9的表达()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年05期
页码:
622-625
栏目:
应用研究
出版日期:
2018-09-20

文章信息/Info

Title:
Dynamic changes of IL-9 in development of colitis associated colorectal cancer in mice
作者:
孙正路1)张艳停2)徐 刚1)朱 丽1)张晓珊1)崔广林1)
1)郑州大学第二附属医院消化内科 郑州 450014;2)郑州大学第二附属医院神经内科 郑州 450014
Author(s):
SUN Zhenglu1)ZHANG Yanting2)XU Gang1)ZHU Li1)ZHANG Xiaoshan1)CUI Guanglin1)
1)Department of Gastroenterology, the Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014;2)Department of Neurology, the Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014
关键词:
结肠炎相关结直肠癌 IL-9 小鼠
Keywords:
colitis associated colorectal cancer IL-9 mouse
分类号:
R735.3
DOI:
10.13705/j.issn.1671-6825.2018.03.006
摘要:
目的:探讨小鼠结肠炎相关结直肠癌(CAC)发生发展过程中大肠组织白介素9(IL-9)表达的动态变化。方法:60只小鼠随机分为对照组和实验组,每组30只。实验组采用1,2-二甲肼+葡聚糖硫酸钠诱发、构建小鼠CAC模型。2组均于14、18和22周处死10只小鼠并收集大肠标本,计数肿瘤数目并鉴定其病理性质,采用免疫组化法和Western blot分别检测IL-9在大肠组织的表达部位及表达水平。结果:实验组从14周开始出现癌前病变,18~22周肿瘤增多。IL-9在对照组少量表达于上皮和间充质细胞; 在实验组则显著表达于转化的上皮细胞和肿瘤基质细胞,实验组14、18、22周,小鼠大肠组织IL-9的表达逐渐升高,且高于对照组(P<0.01)。结论:IL-9在CAC发生发展过程中升高; IL-9可能是小鼠CAC发病机制中的关键因素。
Abstract:
Aim: To evaluate the role of interleukin 9(IL-9)during the process of colitis associated colorectal cancer(CAC).Methods:Sixty mice were randomly allocated into control group and experiment group with 30 mice in each group.The CAC mouse model was induced in the experiment group by the administration of 1,2-dimethylhydrazine plus dextran sulfate sodium.Ten mice in the 2 groups were sacrificed at the 14th week, 18th week and 22nd week respectively and colorectal specimens were collected. The density of tumors in the colorectal mucosa was quantified and recorded. The expression location and level of IL-9 in the colorectal tissue at different time points were determined by immunohistochemistry(IHC)and Western blot,respectively.Results:The results showed that tumor was appeared in the colorectal mucosa from the 14th week, which increased from the 18th week to the 22nd week in the experiment group. IHC results revealed that in control group IL-9 positive cells were primarily observed in both epithelium and lamina propria compartments; in the experiment group, IL-9 positive cells were predominately located in both transformed epithelial cells and tumor stromal cells. Western blot results showed that the expression level of IL-9 in the experiment group was also increased compared with control in the 14th week,18th week and 22nd week(P<0.01). A gradual increasing trend of IL-9 expression was shown from precancerous lesions to cancerous lesions.Conclusion: The expression of IL-9 is significantly increased during the process of CAC, suggesting that IL-9 may be a key factor in the pathogenesis of CAC in mice.

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备注/Memo

备注/Memo:
【基金项目】国家自然科学基金资助项目(81071969); 河南省创新型科技人才队伍建设项目(C20150009)
【作者简介】崔广林,通信作者,男,1963年5月生,博士,主任医师,教授,研究方向:肠道肿瘤及炎性肠病的发病与治疗,E-mail:guanglin.cui@yahoo.com
更新日期/Last Update: 2018-09-20