[1]秦甜甜,张雪燕,李蕾蕾,等.TW37对食管癌TE-1细胞迁移的影响[J].郑州大学学报(医学版),2018,(06):706-710.[doi:10.13705/j.issn.1671-6825.2018.03.158]
 QIN Tiantian,ZHANG Xueyan,LI Leilei,et al.Effects of TW37 on migration of esophageal cancer TE-1 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(06):706-710.[doi:10.13705/j.issn.1671-6825.2018.03.158]
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TW37对食管癌TE-1细胞迁移的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年06期
页码:
706-710
栏目:
食管癌研究
出版日期:
2018-11-20

文章信息/Info

Title:
Effects of TW37 on migration of esophageal cancer TE-1 cells
作者:
秦甜甜张雪燕李蕾蕾霍峻锋石晓丽王希雅叶小萍杨程宇王 淙
郑州大学药物研究院 郑州 450001
Author(s):
QIN Tiantian ZHANG Xueyan LI Leilei HUO Junfeng SHI Xiaoli WANG Xiya YE Xiaoping YANG Chengyu WANG Cong
College of Pharmacy, Zhengzhou University, Zhengzhou 450001
关键词:
食管癌 TW37 迁移 E-Cadherin Vimentin N-Cadherin TE-1细胞
Keywords:
esophageal cancer TW37 migration E-Cadherin Vimentin N-Cadherin TE-1 cell
分类号:
R735.1
DOI:
10.13705/j.issn.1671-6825.2018.03.158
摘要:
目的:观察TW37对食管癌TE-1细胞迁移的影响。方法:用0.25、0.50、1.00、2.00、4.00、8.00、16.00、32.00和64.00 μmol/L的TW37处理TE-1细胞24、48、72 h,采用MTT法检测细胞增殖。倒置显微镜下观察2、4 μmol/L的TW37作用24 h对TE-1细胞形态的影响。划痕实验检测2、4 μmol/L的TW37作用24、48 h对TE-1细胞迁移的影响。Western blot实验测定2、4 μmol/L TW37作用24 h对TE-1细胞E-Cadherin、Vimentin以及N-Cadherin蛋白表达的影响。均设空白对照。结果:TW37抑制TE-1细胞增殖,呈时间和浓度依赖性。TW37作用后TE-1细胞由原来的梭形变成了卵圆形。TW37能够在较低浓度(2 μmol/L)抑制TE-1细胞迁移,4 μmol/L的TW37抑制迁移的作用更强(P<0.05)。TW37能降低TE-1细胞中Vimentin、N-Cadherin的表达,上调E-Cadherin的表达(P<0.05)。结论:TW37可能通过抑制EMT进程进而抑制TE-1细胞的迁移。
Abstract:
Aim:To observe the effects and possible mechanism of TW37 on the migration of esophageal cancer TE-1 cells.Methods:TE-1 cells were treated with 0.25,0.50,1.00,2.00,4.00,8.00,16.00,32.00,and 64.00 μmol/L TW37 for 24,48,and 72 h, respectively, and MTT assay was used to detect cell proliferation. TE-1 cells were treated by 2, 4 μmol/L TW37 for 24 h, the morphology of TE-1 cells was recorded by inverted microscope, and the expressions of E-Cadherin, Vimentin and N-Cadherin were measured by Western blot. TE-1 cells were treated by 2, 4 μmol/L TW37 for 24,48 h,respectively, and cell migration was detected by wound healing assay.Results:TW37 significantly inhibited the proliferation of TE-1 cells in a time-and concentration-dependent manner.TE-1 cells changed from the original shuttle into an oval-shape after being treated by TW37.TW37 could inhibit the migration of TE-1 cells at a lower concentration, which was stronger with higher concentration(P<0.05). Western blot results confirmed that TW37 could significantly reduce the expressions of Vimentin and N-Cadherin,and up-regulate that of E-Cadherin(P<0.05).Conclusion:TW37 might inhibit the migration of TE-1 cells by regulating the expressions of EMT related proteins.

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备注/Memo

备注/Memo:
【基金项目】国家自然科学基金面上项目(81430085); 河南省基础与前沿技术研究项目(152300410030); 河南省高等学校重点科研项目(18A350013, 14B350011)
【作者简介】王淙,通信作者,女,1972年10月生,博士,副教授,研究方向:肿瘤药理,E-mail: wangcong@zzu.edu.cn
更新日期/Last Update: 2018-11-20