[1]张艳停),吕 杰),赵 雪),等.实验性自身免疫性重症肌无力大鼠多肌群SNX17基因的表达[J].郑州大学学报(医学版),2018,(06):715-719.[doi:10.13705/j.issn.1671-6825.2018.03.128]
 ZHANG Yanting),LYU Jie),ZHAO Xue),et al.Analysis of SNX17 gene expression in multi-muscle group of active immunized EAMG rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(06):715-719.[doi:10.13705/j.issn.1671-6825.2018.03.128]
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实验性自身免疫性重症肌无力大鼠多肌群SNX17基因的表达()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年06期
页码:
715-719
栏目:
论著
出版日期:
2018-11-20

文章信息/Info

Title:
Analysis of SNX17 gene expression in multi-muscle group of active immunized EAMG rats
作者:
张艳停1)吕 杰2)赵 雪2)张迎娜2)方 华2)李倩如3)杜 英3)张清勇4)杨俊红5)张运克6)高 峰2)李 昕1)
1)郑州大学第二附属医院神经内科 郑州 450014;2)郑州大学医药科学研究院神经免疫学研究室 郑州 450052;3)郑州大学基础医学院免疫学系 郑州 450001;4)郑州大学第二附属医院普胸外科 郑州 450014;5)河南中医药大学第一附属医院脑病科 郑州 450000;6)河南中医药大学康复医学院康复办公室 郑州 450000
Author(s):
ZHANG Yanting1)LYU Jie2)ZHAO Xue2)ZHANG Yingna2)FANG Hua2)LI Qianru3)DU Ying3)ZHANG Qingyong4)YANG Junhong5)ZHANG Yunke6)GAO Feng2)LI Xin1)
1)Department of Neurology, the Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014;2)Department of Neuroimmunology, Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450052;3)Department of Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001;4)Department of Thoracic Surgery, the Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014;5)Department of Encephalopathy,the First Affiliated Hospital, Henan University of Traditional Chinese Medicine, Zhengzhou 450000;6)Department of Rehabilitation,Institute of Rehabilitation Medicine,Henan University of Traditional Chinese Medicine, Zhengzhou 450000
关键词:
分拣微管连接蛋白17 主动免疫 实验性自身免疫性重症肌无力 神经肌肉接头 大鼠
Keywords:
sorting nexin 17 active immunization experimental autoimmune myasthenia gravis neuromuscular junction rat
分类号:
R746.1
DOI:
10.13705/j.issn.1671-6825.2018.03.128
摘要:
目的:探讨实验性自身免疫性重症肌无力(EAMG)大鼠多肌群分拣微管连接蛋白17(SNX17)的表达特点。方法:采用主动免疫法构建EAMG大鼠模型(EAMG组)并进行评估,以正常大鼠作对照(正常对照组),利用荧光定量PCR和Western blot检测2组大鼠胸锁乳突肌、膈肌、咬肌、心肌、肋间肌、前肢肌、腓肠肌和眼肌等8组肌群中SNX17、nAChR mRNA和蛋白含量变化。结果:成功构建了EAMG大鼠模型。与正常对照组相比,EAMG组大鼠心肌、肋间肌中SNX17 mRNA的表达升高(P<0.05),膈肌中SNX17 mRNA的表达降低(P<0.05); 胸锁乳突肌、腓肠肌和眼肌中nAChR mRNA的表达降低(P<0.05),心肌中nAChR mRNA的表达升高(P<0.05); 咬肌、心肌和腓肠肌中SNX17蛋白含量升高(P<0.05),胸锁乳突肌、膈肌中降低(P<0.05)。EAMG组腓肠肌中SNX17 mRNA与 nAChR mRNA的表达呈负相关(r=-0.773,P=0.035),而在心肌中呈正相关(r=0.947,P=0.012)。结论:SNX17可能是神经肌肉接头处的关键蛋白。
Abstract:
Aim:To investigate the expression characteristics of sorting nexin 17(SNX17)in experimental autoimmune myasthenia gravis(EAMG)rats.Methods:EAMG rat model was constructed and evaluated by active immunization.Western blot and qRT-PCR were used to compare the changes of SNX17, nAChR contents in sternocleidomastoid, diaphragm, masseter, myocardial, intercostal muscle, forelimb muscle, gastrocnemius and eye muscle between EAMG group and control group.Results:The EAMG rats were successfully constructed. The expression level of SNX17 mRNA in the myocardial, and intercostal muscle of EAMG group was significantly higher than that of control group(P<0.05), and was lower in the diaphragm(P<0.05). The expression level of nAChR mRNA in the sternocleidomastoid,gastrocnemius and eye muscle of EAMG group was significantly lower than that of the control group(P<0.05),and was significantly higher in the myocardial muscle(P<0.05).The expression level of SNX17 protein in the masseter, myocardial, gastrocnemius of EAMG group was significantly higher than that of control group.The expression level of SNX17 protein in the diaphragm and sternocleidomastoid muscle was significantly lower than that of control group(P<0.05).The expression of SNX17 mRNA in the gastrocnemius muscle was negatively correlated with the expression of nAChR mRNA in the EAMG group(r=-0.773,P=0.035), and were positively correlated in the myocardial tissue(r=0.947,P=0.012).Conclusion:SNX17 may be a key protein in neuromuscular junctions.

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备注/Memo

备注/Memo:
【基金项目】国家自然科学基金资助项目(81471545); 河南省高等学校重点科研项目计划(16A320010,17A320046); 河南省科技攻关计划项目(172102310298); 河南省高校科技创新团队支持计划(16IRTSTHN022)
【作者简介】高峰,通信作者,男,1970年12月生,博士,研究员,研究方向:神经免疫疾病的机制与临床,E-mail:gaoyuanshan@126.com; 李昕,通信作者,男,1975年12月生,博士,副主任医师,研究方向:脑血管病及自身免疫性疾病,E-mail:lizn999@126.com
更新日期/Last Update: 2018-11-20