[1]王晓慧),刁长英),谢艺林),等.THBS1表达上调对肺腺癌细胞增殖、凋亡和细胞周期的影响[J].郑州大学学报(医学版),2018,(06):730-733.[doi:10.13705/j.issn.1671-6825.2018.04.088]
 WANG Xiaohui),DIAO Changying),XIE Yilin),et al.Effects of up-regulating THBS1 expression on proliferation, apoptosis and cell cycle of lung adenocarcinoma cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(06):730-733.[doi:10.13705/j.issn.1671-6825.2018.04.088]
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THBS1表达上调对肺腺癌细胞增殖、凋亡和细胞周期的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年06期
页码:
730-733
栏目:
论著
出版日期:
2018-11-20

文章信息/Info

Title:
Effects of up-regulating THBS1 expression on proliferation, apoptosis and cell cycle of lung adenocarcinoma cells
作者:
王晓慧1)刁长英1)谢艺林1)刘亚清1)高献争1)韩 静1)李晟磊1)刘红涛2)
1)郑州大学第一附属医院病理科 郑州 450052;2)郑州大学生命科学院细胞生物学研究室 郑州 450001
Author(s):
WANG Xiaohui1) DIAO Changying1) XIE Yilin1) LIU Yaqing1)GAO Xianzheng1)HAN Jing1)LI Shenglei1)LIU Hongtao2)
1)Department of Pathology,the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052; 2)Laboratory for Cell Biology,College of Life Sciences,Zhengzhou University,Zhengzhou 450001
关键词:
血小板凝血酶1 肺腺癌 细胞增殖 细胞凋亡 细胞周期
Keywords:
thrombospondin 1 lung adenocarcinoma cell proliferation cell apoptosis cell cycle
分类号:
R734.2
DOI:
10.13705/j.issn.1671-6825.2018.04.088
摘要:
目的:探讨血小板凝血酶蛋白1(THBS1)表达上调对肺腺癌细胞增殖、凋亡和细胞周期的影响。方法:采用实时荧光定量PCR检测肺腺癌细胞株A549、H1299、H358、H1975、PC9、PG49和Calu3中THBS1 mRNA的表达量; 构建真核表达载体pcDNA3.1-THBS1,以脂质体转染至THBS1 mRNA表达量最低的H1975细胞中,实时荧光定量PCR检测THBS1 mRNA表达水平,CCK-8法检测细胞增殖能力,流式细胞技术分析细胞凋亡及细胞周期分布情况。以未转染和转染空质粒pcDNA3.1的H1975细胞作对照。结果:不同肺腺癌细胞株中THBS1 mRNA表达量不同,H1975细胞中表达最低。与未转染和转染空质粒的H1975细胞比较,转染pcDNA3.1-THBS1的H1975细胞THBS1 mRNA表达上调(P<0.05),细胞增殖能力增强(P<0.05),细胞凋亡率降低(P<0.05),细胞周期进程被促进(P<0.05)。结论:THBS1与肺腺癌细胞的生物学行为关系密切,有可能成为肺腺癌的治疗靶点。
Abstract:
Aim:To investigate the effects of thrombospondin 1(THBS1)expression up-regulation on proliferation, apoptosis and cell cycle of lung adenocarcinoma cells.Methods:The expression levels of THBS1 mRNA in lung adenocarcinoma cell lines A549, H1299, H358, H1975, PC9, PG49 and Calu3 were detected by real-time quantitative PCR(qRT-PCR). Eukaryotic expression vector pcDNA3.1-THBS1 was constructed and transfected into H1975 cells by liposomes.qRT-PCR was used to detect the expression of THBS1 mRNA, CCK-8 kit was used to detect the proliferation,and flow cytometry was used to investigate the apoptosis and cell cycle distribution. The H1975 cells without transfection or transfected with pcDNA3.1 were the controls.Results:The expression levels of THBS1 mRNA in different lung adenocarcinoma cell lines were different, and the lowest was in H1975 cell line.Compared with the controls,the expression of THBS1 mRNA in H1975 cells transfected with pcDNA3.1-THBS1 was higher,proliferation ability was increased,the apoptosis rate decreased,and the cell cycle progression was promoted significantly(P<0.05).Conclusion:THBS1 protein is related with the biological behavior of lung adenocarcinoma cells, and may be a treatment target.

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备注/Memo

备注/Memo:
【基金项目】国家自然科学基金资助项目(81372677); 河南省基础与前沿项目(132300410073); 河南省自然科学基金资助项目(182300410377); 河南省高等学校重点科研项目(17A180016)
【作者简介】李晟磊,通信作者,男,1975 年10月生,博士,主任医师,研究方向:肿瘤病理,E-mail: 13598423254@126.com
更新日期/Last Update: 2018-11-20