[1]潘亚婷,刘慧兵,王立波,等.猪主动脉瓣膜中NADPH氧化酶2和4的表达[J].郑州大学学报(医学版),2018,(06):734-738.[doi:10.13705/j.issn.1671-6825.2018.03.009]
 PAN Yating,LIU Huibing,WANG Libo,et al.Expressions of NADPH oxidase 2 and 4 in normal porcine aortic valves[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(06):734-738.[doi:10.13705/j.issn.1671-6825.2018.03.009]
点击复制

猪主动脉瓣膜中NADPH氧化酶2和4的表达()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年06期
页码:
734-738
栏目:
论著
出版日期:
2018-11-20

文章信息/Info

Title:
Expressions of NADPH oxidase 2 and 4 in normal porcine aortic valves
作者:
潘亚婷刘慧兵王立波王 蕾冷小敏吕 诚王学惠赵国安张 敏
新乡医学院第一附属医院心内科; 新乡医学院心血管病诊疗中心 河南卫辉 453100
Author(s):
PAN Yating LIU Huibing WANG Libo WANG Lei LENG Xiaomin LYU ChengWANG Xuehui ZHAO Guoan ZHANG Min
Department of Cardiology, the First Affiliated Hospital,Xinxiang Medical University; Center of Cardiovascular Disease,Xinxiang Medical University, Weihui, Henan 453100
关键词:
NADPH氧化酶 主动脉瓣膜 间质细胞 内皮细胞
Keywords:
NADPH oxidase swine aortic valve interstitial cell endothelial cell
分类号:
R542.52
DOI:
10.13705/j.issn.1671-6825.2018.03.009
摘要:
目的:检测NADPH氧化酶(Nox)在正常猪主动脉瓣膜及培养的瓣膜间质细胞和内皮细胞中的表达。方法:把猪主动脉瓣膜分为尖部、中部和根部,并从完整瓣膜中分离培养瓣膜间质细胞和内皮细胞。通过免疫荧光法鉴定间质细胞和内皮细胞的表型及组织中Nox主要亚型Nox2和Nox4的表达,采用Real-time PCR和Western blot方法检测瓣膜尖部、中部和根部以及间质细胞和内皮细胞中Nox2和Nox4的表达。结果:与尖部和中部相比,猪主动脉瓣膜根部Nox2和Nox4蛋白表达水平升高(P<0.05); 内皮细胞中Nox4 mRNA和蛋白的表达水平高于间质细胞(P<0.05),但间质细胞中Nox2蛋白表达水平高于内皮细胞(P<0.05)。结论:Nox在猪主动脉瓣膜的生理性表达具有组织和细胞特异性。
Abstract:
Aim:To explore the distribution and expression profiling of NADPH oxidases(Nox)in normal porcine aortic valves.Methods:The porcine aortic valves were divided into three parts: the tip, middle,and root. Primary valvular interstitial cell(VIC)and endothelial cell(EC)were isolated, cultured,and identified. The mRNA and protein levels of main Nox isoforms Nox2 and Nox4 and their distribution in aortic valves and cells were evaluated by Real-time PCR, Western blot,and immunofluorescence,respectively.Results:The protein levels of Nox2 and Nox4 were both significantly higher in the root of valves than those of in the tip and middle parts(P<0.05). Compared with VIC, the endothelial Nox4 mRNA and protein levels were markedly higher, but Nox2 protein was significantly lower in EC(P<0.05).Conclusion:The expressions of Nox2 and Nox4 in normal porcine aortic valves are tissue and cell-specific.

参考文献/References:

[1] LINDMAN BR, CLAVEL MA, MATHIEU P, et al. Calcific aortic stenosis[J].Nat Rev Dis Primers, 2016,2:16006
[2] RUTKOVSKIY A,MALASHICHEVA A,SULLIVAN GA,et al.Valve interstitial cells: the key to understanding the pathophysiology of heart valve calcification[J].J Am Heart Assoc,2017,6(9):e006339
[3] BRANCHETTI E,SAINGER R,POGGIO P,et al.Antioxidant enzymes reduce DNA damage and early activation of valvular interstitial cells in aortic valve sclerosis[J].Arterioscler Thromb Vasc Biol,2013,33(2):E66
[4] ZHANG M,PERINO A,GHIGO A,et al.NADPH oxidases in heart failure: poachers or gamekeepers?[J].Antioxid Redox Signal,2013,18(9):1024
[5] ZHANG M,MONGUE-DIN H,MARTIN D,et al.Both cardiomyocyte and endothelial cell Nox4 mediate protection against hemodynamic overload-induced remodelling[J].Cardiovasc Res,2018,114(3):401
[6] HUK DJ,AUSTIN BF,HORNE TE,et al.Valve endothelial cell-derived Tgfβ1 signaling promotes nuclear localization of Sox9 in interstitial cells associated with attenuated calcification[J].Arterioscler Thromb Vasc Biol,2016,36(2):328
[7] HOLMSTROEM KM,FINKEL T.Cellular mechanisms and physiological consequences of redox-dependent signalling[J].Nat Rev Mol Cell Biol,2014,15(6):411
[8] LAMBETH JD,NEISH AS.Nox enzymes and new thinking on reactive oxygen: a double-edged sword revisited[J].Annu Rev Pathol,2014,9:119
[9] ZHANG M,PROSSER BL,BAMBOYE MA,et al.Contractile function during angiotensin-Ⅱactivation:increased Nox2 activity modulates cardiac calcium handling via phospholamban phosphorylation[J].J Am Coll Cardiol,2015,66(3):261
[10]MURDOCH CE,CHAUBEY S,ZENG LF,et al.Endothelial NADPH oxidase-2 promotes interstitial cardiac fibrosis and diastolic dysfunction through proinflammatory effects and endothelial-mesenchymal transition[J].J Am Coll Cardiol,2014,63(24):2734
[11]SIRKER A,MURDOCH CE,PROTTI A,et al.Cell-specific effects of Nox2 on the acute and chronic response to myocardial infarction[J].J Mol Cell Cardiol,2016,98:11
[12]MONGUE-DIN H,PATEL AS,LOOI YH,et al.NADPH oxidase-4 driven cardiac macrophage polarization protects against myocardial infarction-induced remodeling[J].JACC Basic Transl Sci,2017,2(6):688
[13]SANTOS CX,HAFSTAD AD,BERETTA M,et al.Targeted redox inhibition of protein phosphatase 1 by Nox4 regulates eIF2α-mediated stress signaling[J].EMBO J,2016,35(3):319
[14]SMYRNIAS I,ZHANG X,ZHANG M,et al.Nicotinamide adenine dinucleotide phosphate oxidase-4-dependent upregulation of nuclear factor erythroid-derived 2-like 2 protects the heart during chronic pressure overload[J].Hypertension,2015,65(3):547
[15]SCHRÖDER K,ZHANG M,BENKHOFF S,et al.Nox4 is a protective reactive oxygen species generating vascular NADPH oxidase[J].Circ Res,2012,110(9):1217
[16]ZHANG M,BREWER AC,SCHROEDER K,et al.NADPH oxidase-4 mediates protection against chronic load-induced stress in mouse hearts by enhancing angiogenesis[J].Proc Natl Acad Sci U S A,2010,107(42):18121
[17]ZHANG M,SHAH AM.ROS signalling between endothelial cells and cardiac cells[J].Cardiovasc Res,2014,102(2):249

备注/Memo

备注/Memo:
【基金项目】国家自然科学基金资助项目(81470506)
【作者简介】张敏,通信作者,男,1970年8月生,博士,教授,研究方向:活性氧与心血管疾病,E-mail:minzhang000@126.com
更新日期/Last Update: 2018-11-20