[1]朱睿明,邢方岚,张起顺,等.miRNA-21过表达对乳鼠星形胶质细胞凋亡及Wnt/β-catenin信号通路相关蛋白表达的影响[J].郑州大学学报(医学版),2018,(06):768-771.[doi:10.13705/j.issn.1671-6825.2018.07.006]
 ZHU Ruiming,XING Fanglan,ZHANG Qishun,et al.Effects of miRNA-21 overexpression on apoptosis and expressions of Wnt/β-catenin signaling pathway related proteins in astrocytes from neonate rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(06):768-771.[doi:10.13705/j.issn.1671-6825.2018.07.006]
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miRNA-21过表达对乳鼠星形胶质细胞凋亡及Wnt/β-catenin信号通路相关蛋白表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年06期
页码:
768-771
栏目:
应用研究
出版日期:
2018-11-20

文章信息/Info

Title:
Effects of miRNA-21 overexpression on apoptosis and expressions of Wnt/β-catenin signaling pathway related proteins in astrocytes from neonate rats
作者:
朱睿明邢方岚张起顺赵 俊
河南大学淮河医院康复医学科 河南开封 475000
Author(s):
ZHU RuimingXING FanglanZHANG QishunZHAO Jun
Department of Rehabilitation Medicine, Huaihe Hospital of Henan University, Kaifeng,Henan 475000
关键词:
星形胶质细胞 miRNA-21 凋亡 Wnt/β-catenin信号通路
Keywords:
astrocyte miRNA-21 apoptosis Wnt/β-catenin signaling pathway
分类号:
R445.2
DOI:
10.13705/j.issn.1671-6825.2018.07.006
摘要:
目的:探讨miRNA-21(miR-21)过表达对乳鼠星形胶质细胞(AS)凋亡及Wnt/β-catenin信号通路相关蛋白表达的影响。方法:AS分为3组,空白对照组、H2O2组(400 μmol/L的H2O2处理AS 24 h)和miR-21+H2O2组(miR-21 mimics转染AS 24 h后用400 μmol/L的H2O2处理细胞24 h)。采用qRT-PCR检测3组细胞miR-21的表达,流式细胞术检测细胞凋亡,Western blot检测β-catenin、c-myc和Bax蛋白的表达。结果:与空白对照组比较,H2O2组miR-21表达降低,细胞凋亡率升高,β-catenin和c-myc表达降低,Bax表达升高(P<0.05); 与H2O2组比较,miR-21+H2O2组miR-21表达升高,细胞凋亡率降低,β-catenin和c-myc蛋白表达升高,Bax蛋白表达降低(P<0.05)。结论:过表达miR-21可降低AS凋亡,其机制可能与Wnt/β-catenin信号通路的激活有关。
Abstract:
Aim:To investigate the effect of miRNA-21(miR-21)gene overexpression on the apoptosis of astrocytes and the expressions of Wnt/β-catenin signaling pathway-related proteins.Methods:Astrocytes were divided into 3 groups,namely, blank control group, H2O2 group and miR-21+H2O2 group. qRT-PCR was used to detect the expression of miR-21 mRNA,flow cytometry was used to detect cell apoptosis,and Western blot was used to detect the expressions of β-catenin, c-myc and Bax protein.Results:Compared with blank control group,the expression of miR-21 in the H2O2 group decreased, the apoptosis rate increased, the expressions of β-catenin and c-myc decreased, and the expression of Bax increased(P<0.05); compared with the H2O2 group, the miR-21 expression in the miR-21+H2O2 group increased, the apoptosis rate decreased, the expressions of β-catenin and c-myc increased, and the expression of Bax decreased(P<0.05).Conclusion:Overexpression of miR-21 could reduce the apoptosis of astrocytes, which may be related to the activation of Wnt/β-catenin signaling pathway.

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备注/Memo

备注/Memo:
【基金项目】河南省科技攻关项目(182102311242)
【作者简介】赵俊,通信作者,女,1979年10月生,硕士,副主任医师,研究方向:脑血管疾病及神经康复,E-mail:vhdengv559@163.com
更新日期/Last Update: 2018-11-20