[1]刘继攀,孙 伟,赵学荣,等.miR-101对结直肠癌细胞增殖、凋亡及KIF14蛋白表达的影响[J].郑州大学学报(医学版),2018,(06):776-779.[doi:10.13705/j.issn.1671-6825.2018.11.003]
 LIU Jipan,SUN Wei,ZHAO Xuerong,et al.Effect of miR-101 on proliferation,apoptosis and KIF14 expression of colorectal cancer cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(06):776-779.[doi:10.13705/j.issn.1671-6825.2018.11.003]
点击复制

miR-101对结直肠癌细胞增殖、凋亡及KIF14蛋白表达的影响()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年06期
页码:
776-779
栏目:
应用研究
出版日期:
2018-11-20

文章信息/Info

Title:
Effect of miR-101 on proliferation,apoptosis and KIF14 expression of colorectal cancer cells
作者:
刘继攀孙 伟赵学荣李炳茂王成君
衡水市哈励逊国际和平医院(衡水市人民医院)普外二科 河北衡水053000
Author(s):
LIU Jipan SUN Wei ZHAO Xuerong LI BingmaoWANG Chengjun
Department Ⅱ of General Surgery, Hengshui Harrison International Peace Hospital(Hengshui People's Hospital), Hengshui, Hebei 053000
关键词:
miR-101 结直肠癌 KIF14 凋亡
Keywords:
miR-101 colorectal cancer KIF14 apoptosis
分类号:
R735.3
DOI:
10.13705/j.issn.1671-6825.2018.11.003
摘要:
目的:研究miR-101对结直肠癌细胞增殖、凋亡及驱动蛋白家族蛋白14(KIF14)表达的影响。方法:利用靶基因预测库预测miR-101与KIF14的靶向关系,并采用双荧光素酶报告实验鉴定。采用实时荧光定量PCR和Western blot法测定结直肠癌组织和细胞中miR-101和KIF14蛋白的表达。在结直肠癌HT29细胞中转染miR-101 模拟物,MTT法测定细胞增殖情况,Annexin V-FITC/PI双染法检测细胞凋亡率,Western blot法测定KIF14蛋白的表达。结果:双荧光素酶报告实验结果显示,miR-101与KIF14存在靶向关系。结直肠癌组织和细胞中miR-101表达下调,KIF14表达上调(P<0.05)。miR-101 模拟物可以明显抑制HT29细胞的增殖能力并促进其凋亡,同时下调KIF14蛋白表达水平(P<0.05)。结论:miR-101可靶向作用于KIF14而影响结直肠癌细胞的增殖和凋亡。
Abstract:
Aim: To study the effect of miR-101 on proliferation,apoptosis and KIF14 expression of colorectal cancer cells.Methods: Target gene prediction library was used to predict the targeting relationship between miR-101 and KIF14, and double luciferase reporter assay was used to identify the relationship.The expressions of miR-101 and KIF14 in colorectal cancer tissue and colorectal cancer cells were detected by qRT-PCR and Western blot.miR-101 mimics was transfected into HT29 cells,and then cell proliferation was measured by MTT,apoptosis was detected by Annexin V-FITC/PI staining,Western blot was used to detect the KIF14 protein expression.Results: The double luciferase reporter assay showed that there was a target relationship between miR-101 and KIF14.miR-101 was downregulated and KIF14 was upregulated in colorectal cancer tissue and colorectal cancer cells(P<0.05).miR-101 mimics significantly inhibited the proliferation and KIF14 protein expression,and promote apoptosis in HT29 cells(P<0.05).Conclusion: miR-101 could inhibit proliferation and induce apoptosis of colorectal cancer cells by targeting KIF14.

参考文献/References:

[1] ZHANG S,REN H,CHEN MW.miRNA-204 suppresses human non-small cell lung cancer by targeting ATF2[J].Chest,2016,149(Suppl 4):300A
[2] ZHENG M,JIANG YP,CHEN W,et al.Snail and Slug collaborate on EMT and tumor metastasis through miR-101-mediated EZH2 axis in oral tongue squamous cell carcinoma[J].Oncotarget,2015,6(9):6797
[3] CUI Y,ZHANG FM,ZHU CK,et al.Upregulated lncRNA SNHG1 contributes to progression of nonsmall cell lung cancer through inhibition of miR-101-3p and activation of Wnt/beta-catenin signaling pathway[J].Oncotarget,2017,8(11):17785
[4] QIU HL,DENG SZ,LI C,et al.High expression of KIF14 is associated with poor prognosis in patients with epithelial ovarian cancer[J].Eur Rev Med Pharmacol Sci,2017,21(2):239
[5] O'HARE M,SHADMAND M,SULAIMAN RS,et al.Kif14 overexpression accelerates murine retinoblastoma development[J].Int J Cancer,2016,139(8):1752
[6] HE W,FENG JF,ZHANG Y,et al.microRNA-186 inhibits cell proliferation and induces apoptosis in human esophageal squamous cell carcinoma by targeting SKP2[J].Lab Invest,2016,96(3):317
[7] YAN W,CHEN JQ,CHEN ZY,et al.MicroRNA-506-3p regulates neural stem cell proliferation and differentiation through targeting TCF3[J].Gene,2016,593(1):193
[8] 雷伟琦,胡谦,王思远,等.miR-101在结直肠癌中的研究进展[J].中国普通外科杂志,2016,25(4):592
[9] CAO K,LI JJ,ZHAO Y,et al.miR-101 inhibiting cell proliferation, migration and invasion in hepatocellular carcinoma through downregulating girdin[J].Mol Cells,2016,39(2):96
[10]CHEN S,WANG HY,WOOI LN,et al.Radiosensitizing effects of ectopic miR-101 on non-small-cell lung cancer cells depend on the endogenous miR-101 level[J].Int J Radiat Oncol Biol Phys,2011,81(5):1524
[11]YAO ZS,LI C,LIANG D,et al.Diagnostic and prognostic implications of serum miR-101 in osteosarcoma[J].Cacer biomark,2018,22(1):127
[12]WANG H,WANG L,ZHANG G,et al.MALAT1/miR-101-3p/MCL1 axis mediates cisplatin resistance in lung cancer[J].Oncotarget,2018,9(7):7501
[13]林松,邵楠楠,范磊,等. miR-101对骨肉瘤MG-63细胞增殖和凋亡影响机制探讨[J].中华肿瘤防治杂志,2014,21(6):428
[14]ZHANG S,WANG M,LI Q,et al.MiR-101 reduces cell proliferation and invasion and enhances apoptosis in endometrial cancer via regulating PI3K/Akt/mTOR[J].Cancer Biomark,2017,21(1):1 79
[15]MA YY,LUO W,BUNCH BL,et al.1,25D(3)differentially suppresses bladder cancer cell migration and invasion through the induction of miR-101-3p[J].Oncotarget,2017,8(36):60080
[16]COHEN Y,GUTWEIN O,GARACH-JEHOSHUA O,et al.The proliferation arrest of primary tumor cells out-of-niche is associated with widespread downregulation of mitotic and transcriptional genes[J].Hematology,2014,19(5):286
[17]THÉRIAULT BL,BASAVARAJAPPA HD,LIM H,et al.Transcriptional and epigenetic regulation of KIF14 overexpression in ovarian cancer[J].PLoS One,2014,9(3):e91540
[18]李晓娜,韩雪,杨涛.KIF14过表达与肝细胞肝癌的发生密切相关[J].河北医科大学学报,2014,35(7):761
[19]MADHAVAN J,MITRA M,MALLIKARJUNAK,et al.KIF14 and E2F3 mRNA expression in human retinoblastoma and its phenotype association[J].Mol Vis,2009,15:235

相似文献/References:

[1]梁高峰),李俊生),何向峰),等.基于高通量测序的结直肠癌miRNA表达谱分析[J].郑州大学学报(医学版),2015,(05):618.
 LIANG Gaofeng)),LI Junsheng),HE Xiangfeng),et al.Analysis of miRNA expression profiles in colorectal cancer based on high-throughput sequencing[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(06):618.
[2]郑 立△,史朝辉.低位梗阻性左半结直肠癌经肛肠梗阻导管清洗减压与术中结肠灌洗效果比较[J].郑州大学学报(医学版),2016,(04):552.
[3]罗真真),张 震),乔亚敏),等.萝卜硫素对结直肠癌SW620细胞增殖、凋亡及迁移的影响*[J].郑州大学学报(医学版),2017,(03):281.[doi:10.13705/j.issn.1671-6825.2017.03.010]
 LUO Zhenzhen),ZHANG Zhen),QIAO Yamin),et al.Effects of sulforaphane on proliferation,apoptosis and migration of SW620 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2017,(06):281.[doi:10.13705/j.issn.1671-6825.2017.03.010]
[4]郑紫恒),周英发),任景丽),等.hMLH1、hMSH2、hMSH6在中青年散发性结直肠癌中的表达及对预后的影响[J].郑州大学学报(医学版),2018,(02):165.[doi:10.13705/j.issn.1671-6825.2017.07.50]
 ZHENG Ziheng),ZHOU Yingfa),REN Jingli),et al.Expressions of hMLH1,hMSH2 and hMSH6 in young and middle-aged patients with sporadic colorectal cancer and its effect on prognosis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(06):165.[doi:10.13705/j.issn.1671-6825.2017.07.50]

备注/Memo

备注/Memo:
【基金项目】河北省中医药类科研指导计划项目(2015327); 河北省名中医李炳茂传承工作室建设项目(冀中药函[2015]35号)
【作者简介】刘继攀,通信作者,男,1976年9月生,硕士研究生,副主任医师,研究方向:结直肠外科,E-mail:ljp13932856919@163.com
更新日期/Last Update: 2018-11-20