[1]王乐峰),周 华),刘鲁昱).葛根素对多发性骨髓瘤细胞增殖和凋亡的影响[J].郑州大学学报(医学版),2018,(06):824-827.[doi:10.13705/j.issn.1671-6825.2018.03.041]
 WANG Lefeng),ZHOU Hua),LIU Luyu).Effects of puerarin on proliferation and apoptosis of multiple myeloma H929 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2018,(06):824-827.[doi:10.13705/j.issn.1671-6825.2018.03.041]
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葛根素对多发性骨髓瘤细胞增殖和凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2018年06期
页码:
824-827
栏目:
应用研究
出版日期:
2018-11-20

文章信息/Info

Title:
Effects of puerarin on proliferation and apoptosis of multiple myeloma H929 cells
作者:
王乐峰1)周 华2)刘鲁昱3)
1)新汶矿业集团中心医院骨二科 山东泰安 271200;2)山东大学附属省立医院血管外科 济南 250021;3)山东省立第三医院急诊科 济南 250000
Author(s):
WANG Lefeng1)ZHOU Hua2)LIU Luyu3)
1)The Second Department of Orthopedics,Xinwen Mining Group Central Hospital,Tai'an,Shandong 271200;2)Department of Vascular Surgery,Provincial Hospital Affiliated to Shandong University,Jinan 250021;3)Department of Emergency,Shandong Provincial Third Hospital, Jinan 250000
关键词:
葛根素 多发性骨髓瘤 细胞增殖 细胞凋亡
Keywords:
puerarin multiple myeloma cell proliferation cell apoptosis
分类号:
R738.1
DOI:
10.13705/j.issn.1671-6825.2018.03.041
摘要:
目的:探讨葛根素对多发性骨髓瘤细胞增殖和凋亡的影响及其机制。方法:取对数生长期的H929细胞,经0、20、40和80 μmol/L葛根素处理48 h,采用CCK-8法检测细胞增殖情况,流式细胞仪检测细胞的凋亡情况,Western blot检测细胞中AKT、p-AKT、Bcl-2和Cleaved Caspase-3蛋白的表达情况。结果:葛根素浓度依赖性地抑制H929细胞增殖,降低p-AKT和Bcl-2蛋白表达,诱导细胞凋亡,提高Cleaved Caspase-3蛋白表达(P<0.05)。结论:葛根素能够抑制H929细胞增殖并诱导细胞凋亡,其作用机制可能与抑制PI3K/AKT信号通路有关。
Abstract:
Aim:To investigate the effects of puerarin on proliferation and apoptosis of multiple myeloma H929 cells and its mechanism.Methods:H929 cells in logarithmic growth phase were treated with 0, 20, 40 and 80 μmol/L puerarin for 48 h.The proliferation of cells was detected by CCK-8 assay, apoptosis was detected by FCM, and the expressions of AKT, p-AKT, Bcl-2 and Cleaved caspase-3 protein in cells were detected by Western blot.Results:Puerarin inhibited the proliferation of H929 cells and the expressions of p-AKT and Bcl-2 protein, and induced apoptosis and increased Cleaved Caspase-3 protein expression in a concentration dependent manner(P<0.05).Conclusion:Puerarin can inhibit H929 cell proliferation,induce cell apoptosis,and the mechanism may be related to the inhibition of the PI3K/AKT signaling pathway.

参考文献/References:

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备注/Memo

备注/Memo:
【基金项目】山东省自然科学基金项目(ZR2015HL108)
【作者简介】王乐峰,通信作者,男,1982年1月生,硕士,主治医师,研究方向:骨外科,E-mail:ligv228@163.com
更新日期/Last Update: 2018-11-20