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Inhibition of ERBB2 gene on hepatoma HepG2 cell growth and PI3K/AKT signaling pathway
顾 清张 莉张新星代小松陈和平
四川省医学科学院(四川省人民医院)老年消化科 成都 610072
GU QingZHANG LiZHANG XinxingDAI XiaosongCHEN Heping
Department of Gastroenterology, Sichuan Academy of Medical Sciences(Sichuan Provincial People's Hospital), Chengdu 610072
ERBB2基因 肝癌 HepG2细胞 细胞生长 PI3K/AKT信号通路
ERBB2 gene liver cancer HepG2 cell cell growth PI3K/AKT signaling pathway
目的:探讨抑制ERBB2基因表达对肝癌HepG2细胞生长及PI3K/AKT信号通路的影响。方法:采用脂质体法将ERBB2 siRNA转染至肝癌HepG2细胞,记为实验组(ERBB2-siRNA组),以siRNA 对照转染的细胞记为阴性对照组(Con-siRNA组),将不经任何处理的细胞记为空白对照组。采用qRT-PCR和Western blot法检测转染效率,CCK-8法检测细胞增殖能力,克隆形成实验检测细胞克隆形成能力,流式细胞仪检测细胞周期,Western blot法检测HepG2细胞中增殖细胞核抗原(PCNA)、AKT和p-AKT蛋白表达水平。结果:在HepG2细胞中转染ERBB2 siRNA能够抑制ERBB2的表达(P<0.05)。与Con-siRNA组比较,抑制ERBB2的表达后细胞生长和克隆形成能力明显被抑制,细胞周期发生G0/G1期阻滞,细胞中PCNA和p-AKT蛋白表达下调,差异均有统计学意义(P<0.05)。结论:抑制ERBB2基因表达可抑制肝癌HepG2细胞的生长,其作用机制可能与PI3K/AKT信号通路的激活有关。
Aim:To investigate the effect of inhibiting the expression of ERBB2 gene on the growth of HepG2 cells and PI3K/AKT signaling pathway.Methods:The ERBB2 siRNA was transfected into HepG2 cells by liposome method, which was recorded as the experimental group(ERBB2-siRNA group). The cells transfected with siRNA control were recorded as negative control group(Con-siRNA group), the cells without any treatment were recorded as blank control group. The transfection efficiency was detected by qRT-PCR and Western blot, the cell proliferation ability was detected by CCK-8 method, the cell clone formation ability was detected by colony formation assay, and the cell cycle was detected by flow cytometry. Proliferating cell nuclear antigen(PCNA), AKT and p-AKT protein levels were measured in HepG2 cells by Western blot.Results:Transfection of ERBB2 siRNA in HepG2 cells inhibited the expression of ERBB2(P<0.05). Compared with the Con-siRNA group, the cell growth and colony formation abilities were significantly inhibited after reducing the expression of ERBB2(P<0.05). The cells arrested at G0/G1 phase and the expressions of PCNA and p-AKT proteins in the cells were down-regulated(P<0.05).Conclusion:Inhibition of ERBB2 gene expression could inhibit the growth of HepG2 cells, and its mechanism is related to the activation of PI3K/AKT signaling pathway.


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【基金项目】四川省干部保健委员会项目(川干研2017-236) 【作者简介】顾清,通信作者,女,1980年7月生,硕士,主治医师,研究方向:炎症性肠病及胃肠道肿瘤,E-mail:gongsihui5@163.com
更新日期/Last Update: 2019-01-20