[1]刘欣欣,孙华磊,葛惠娜,等.紫檀芪对2型糖尿病大鼠学习记忆能力及海马氧化应激水平的影响[J].郑州大学学报(医学版),2019,(01):51-54.[doi:10.13705/j.issn.1671-6825.2018.05.023]
 LIU Xinxin,SUN Hualei,GE Huina,et al.Effects of pterostilbene on learning and memory ability and oxidative stress in hippocampus of type 2 diabetic rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(01):51-54.[doi:10.13705/j.issn.1671-6825.2018.05.023]
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紫檀芪对2型糖尿病大鼠学习记忆能力及海马氧化应激水平的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年01期
页码:
51-54
栏目:
应用研究
出版日期:
2019-01-20

文章信息/Info

Title:
Effects of pterostilbene on learning and memory ability and oxidative stress in hippocampus of type 2 diabetic rats
作者:
刘欣欣孙华磊葛惠娜王 腾李文杰
郑州大学公共卫生学院营养与食品卫生学教研室 郑州 450001
Author(s):
LIU Xinxin SUN Hualei GE Huina WANG Teng LI Wenjie
Department of Nutrition and Food Hygiene, College of Public Health, Zhengzhou University, Zhengzhou 450001
关键词:
紫檀芪 2型糖尿病 学习记忆能力 大鼠
Keywords:
pterostilbene type 2 diabetes learning and memory ability rat
分类号:
R151.3
DOI:
10.13705/j.issn.1671-6825.2018.05.023
摘要:
目的:探讨紫檀芪对2型糖尿病大鼠学习记忆能力及海马组织氧化应激水平的影响。方法:100只4周龄雄性SD大鼠适应性喂养1周后,选取10只作为正常对照组,其余大鼠给予高脂高糖饲料喂养。4周后夜间禁食12 h,按40 mg/kg剂量一次性空腹注射链脲佐菌素溶液,制备2型糖尿病大鼠模型。选取造模成功的大鼠40只分为2型糖尿病组、紫檀芪低剂量组、紫檀芪中剂量组、紫檀芪高剂量组,每组10只。紫檀芪干预7周后,采用全自动生化分析仪检测血糖和血清胰岛素,用Morris水迷宫测定大鼠学习记忆能力,用氧化酶试剂盒检测海马组织中SOD活性和MDA水平。结果:与正常对照组相比,2型糖尿病组大鼠血糖、血清胰岛素水平升高(P<0.05); 与2型糖尿病组大鼠相比,紫檀芪低、中剂量组血糖降低,紫檀芪低、中、高剂量组血清胰岛素水平降低(P<0.05)。同时,与正常对照组相比,2型糖尿病组大鼠逃避潜伏期延长,穿越平台次数减少,海马组织SOD活性降低,MDA水平增加(P<0.05); 与2型糖尿病组大鼠相比,紫檀芪低、中、高剂量组逃避潜伏期缩短,穿越平台次数增加,海马组织SOD活性升高,MDA水平降低(P<0.05)。结论:紫檀芪可以改善2型糖尿病大鼠学习记忆能力,提高海马组织SOD 活性,对2型糖尿病大鼠认知障碍具有一定的改善作用。
Abstract:
Aim:To explore the effects of pterostilbene(PTE)on learning and memory ability and oxidative stress in hippocampus of type 2 diabetic rats.Methods:A total of 100 SD rats were randomly allocated into control group(n=10)and test group(n=90). The test group was for making type 2 diabetes model(T2DM), which was induced by combination of high-fat and high-sugar diet combined with streptozotocin(40 mg/kg). Then 40 T2DM rats were randomly allocated into model group, PTE low dose group, PTE middle dose group and PTE high dose group. After administration of PTE for 7 weeks, fasting blood glucose(FBG)and serum insulin(INS)were examined, the learning and memory performance were detected by Morris water maze, and the SOD activity and MDA level were evaluated.Results:Compared with normal rats, the levels of FBG and INS were increased in T2DM group(P<0.05), whereas the level of FBG in PTE(low and middle dose)groups was lower than that of T2DM group(P<0.05), the level of INS in PTE(low, middle and high dose)groups was lower than that of T2DM group(P<0.05). Compared with normal rats, the escape latency extended, the times across the platform shortened, the activity of SOD reduced, and the level of MDA augmented in T2DM group(P<0.05). Moreover, compared with T2DM rats, the escape latency diminished, the times across the platform increased,the activity of SOD augmented, and the level of MDA lessened in PTE(low,middle and high dose)groups(P<0.05).Conclusion:PTE could improve the learning and memory ability and increase SOD activity of type 2 diabetic rats, which might improve the cognitive decline.

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备注/Memo

备注/Memo:
【基金项目】河南省科技创新人才计划项目(154200510010); 河南省科技计划项目(172102310029) 【作者简介】李文杰,通信作者,男,1958年11月生,硕士,教授,研究方向:营养与疾病,E-mail:lwj@zzu.edu.cn
更新日期/Last Update: 2019-01-20