[1]刘春华),王俊轶),银 春),等.非小细胞肺癌细胞中上皮特异性黏附分子的表达及其对细胞增殖和侵袭能力的影响[J].郑州大学学报(医学版),2019,(01):59-63.[doi:10.13705/j.issn.1671-6825.2018.06.118]
 LIU Chunhua),WANG Junyi),YIN Chun),et al.Expression of EpCAM in non-small cell lung cancer cells and its effects on cell proliferation and invasion[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(01):59-63.[doi:10.13705/j.issn.1671-6825.2018.06.118]
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非小细胞肺癌细胞中上皮特异性黏附分子的表达及其对细胞增殖和侵袭能力的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年01期
页码:
59-63
栏目:
应用研究
出版日期:
2019-01-20

文章信息/Info

Title:
Expression of EpCAM in non-small cell lung cancer cells and its effects on cell proliferation and invasion
作者:
刘春华1)王俊轶2)银 春1)张 艳1)姜 琪1)
1)四川绵阳四〇四医院呼吸内科 四川绵阳 621000 2)成都市第三人民医院呼吸与危重症医学科 成都 610031
Author(s):
LIU Chunhua1)WANG Junyi2)YIN Chun1)ZHANG Yan1)JIANG Qi1)
1)Department of Respiratory Medicine, Mianyang No.404 Hospital of Sichuan, Mianyang, Sichuan 621000 2)Department of Respiratory and Critical Care Medicine, Chengdu Third People's Hospital,Chengdu 610031
关键词:
非小细胞肺癌 EpCAM 细胞增殖 侵袭
Keywords:
non-small cell lung cancer EpCAM cell proliferation invasion
分类号:
R734.2
DOI:
10.13705/j.issn.1671-6825.2018.06.118
摘要:
目的:探讨上皮特异性黏附分子(EpCAM)在非小细胞肺癌(NSCLC)细胞中的表达及其对细胞增殖和侵袭能力的影响。方法:采用qRT-PCR和Western blot法检测人正常支气管上皮BEAS-2B细胞和NSCLC H226、A549、PC-9细胞中EpCAM mRNA和蛋白的表达。构建EpCAM低表达的A549细胞系,CCK-8法检测EpCAM对细胞增殖能力的影响,细胞平板克隆实验和Transwell实验检测EpCAM对细胞克隆形成能力和侵袭能力的影响,Western blot法检测EpCAM对细胞中Ki-67和MMP-9蛋白表达的影响。结果:H226、A549、PC-9细胞中EpCAM mRNA和蛋白的表达水平均高于BEAS-2B细胞,表达水平依次为A549>PC-9>H226(P<0.05)。成功干扰A549细胞中EpCAM表达后,细胞的增殖、克隆形成和侵袭能力均减弱,且Ki-67和MMP-9蛋白的表达均降低,与对照组相比,差异均有统计学意义(P<0.05)。结论:EpCAM在NSCLC细胞中高表达; 干扰其表达可能通过下调Ki-67和MMP-9表达抑制A549细胞的增殖和侵袭。
Abstract:
Aim:To investigate the expression of epithelial cell adhesion molecule(EpCAM)in non-small cell lung cancer(NSCLC)cells and its effects on cell proliferation and invasion.Methods:The expressions of EpCAM mRNA and protein in human bronchial epithelial BEAS-2B cells and NSCLC H226, A549 and PC-9 cells were detected by qRT-PCR and Western blot.After construction of EpCAM low expression A549 cell line,the effect of EpCAM on cell proliferation was measured by CCK-8 assay,the effects of EpCAM on cell cloning and invasion abilities were tested by cell clone and Transwell experiments,and the effect of EpCAM on the expression of cell proliferation related protein Ki-67 and MMP-9 protein in cells was examed by Western blot.Results:The expression levels of EpCAM mRNA and protein in H226, A549 and PC-9 cells were significantly higher than that of BEAS-2B cells, and the intensity was A549>PC-9>H226 in turn(P<0.05).After successfully interfering EpCAM expression in A549 cells, the abilities of cell proliferation, cloning and invasion were significantly reduced, and the expressions of Ki-67 and MMP-9 protein were also significantly decreased.Compared with the control group, the difference was statistically significant(P<0.05).Conclusion:EpCAM is highly expressed in NSCLC cells; interfering its expression may inhibit the proliferation and invasion of A549 cells by downregulating the expressions of Ki-67 and MMP-9.

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备注/Memo

备注/Memo:
【基金项目】四川省卫生和计划生育委员会科研计划项目(18PJ403) 【作者简介】银春,通信作者,女,1968年12月生,硕士,主任医师,研究方向:慢性阻塞性肺疾病的基础与临床,E-mail:warnrdkdid66632@163.com
更新日期/Last Update: 2019-01-20