[1]张广云,张海芳,乔明洲.膀胱癌中Galectin-3的表达及其对膀胱癌细胞增殖、凋亡的影响[J].郑州大学学报(医学版),2019,(01):101-105.[doi:10.13705/j.issn.1671-6825.2018.06.041]
 ZHANG Guangyun,ZHANG Haifang,QIAO Mingzhou.Effects of Galectin-3 gene expression on proliferation and apoptosis of bladder cancer cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(01):101-105.[doi:10.13705/j.issn.1671-6825.2018.06.041]
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膀胱癌中Galectin-3的表达及其对膀胱癌细胞增殖、凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年01期
页码:
101-105
栏目:
应用研究
出版日期:
2019-01-20

文章信息/Info

Title:
Effects of Galectin-3 gene expression on proliferation and apoptosis of bladder cancer cells
作者:
张广云张海芳乔明洲
安阳市人民医院泌尿外科 河南安阳 455000
Author(s):
ZHANG GuangyunZHANG HaifangQIAO Mingzhou
Department of Urology,the People's Hospital of Anyang,Anyang,Henan 455000
关键词:
膀胱癌 Galectin-3基因 增殖 凋亡 Notch信号通路
Keywords:
bladder cancer Galectin-3 gene proliferation apoptosis Notch signaling pathway
分类号:
R737.14
DOI:
10.13705/j.issn.1671-6825.2018.06.041
摘要:
目的:探讨Galectin-3在膀胱癌的表达及其对膀胱癌细胞增殖和凋亡的影响。方法:采用Western blot法检测膀胱癌、癌旁正常组织(54例)及SV-HUC-1、T24、5637、BIU-87细胞中Galectin-3蛋白的表达; 转染NC-siRNA、Galectin-3-siRNA1和Galectin-3-siRNA2入膀胱癌T24细胞,未转染任何siRNA作为对照组,转染48 h后采用Western blot法检测各组细胞中Galectin-3蛋白的表达。CCK8及流式细胞术分别检测对照组、NC-siRNA组、Galectin-3-siRNA组细胞存活率和凋亡率; Western blot法检测Notch1、Hes1、Cleaved Caspase-3、PCNA和Ki-67蛋白的表达。结果:膀胱癌组织中Galectin-3的表达高于癌旁正常组织(P<0.001),T24细胞中Galectin-3的表达最高(P<0.05); Galectin-3-siRNA1、Galectin-3-siRNA2组Galectin-3 mRNA及蛋白表达均低于对照组(P<0.05),Galectin-3-siRNA1组的抑制效果更明显。Galectin-3-siRNA组与对照组比较,细胞存活率降低,Notch1、Hes1、PCNA和Ki-67表达降低,细胞凋亡率升高,Cleaved Caspase-3表达升高,差异均有统计学意义(P<0.05)。结论:抑制膀胱癌Galectin-3表达可降低细胞活力,诱导细胞凋亡,其机制可能与Notch信号通路的下调有关。
Abstract:
Aim:To investigate the expression of Galectin-3 gene in bladder cancer and its effect on proliferation and apoptosis of bladder cancer cells.Methods:The expression of Galectin-3 protein in bladder cancer tissue and paracancerous tissue(54 cases)and SV-HUC-1,T24, 5637, BIU-87 cells was detected by Western blot. NC-siRNA and Galectin-3-siRNA1 and Galectin-3-siRNA2 were transfected into bladder cancer T24 cells, and those not transfected with any siRNA was used as control. The expression of Galectin-3 protein was detected by Western blot after transfected for 48 hours.The cells proliferation was detected by CCK8 assay, cell apoptosis was detected by flow cytometry; and the expressions of Ki-67, PCNA, Cleaved Caspase-3, Notch1, and Hes1 proteins in control group,NC-siRNA group,Galectin-3-siRNA group were detected by Western blot.Results:The expression of Galectin-3 in bladder cancer tissue was significantly higher than that in paracancerous tissue(P<0.001). The Galectin-3 expression was highest in T24 cells(P<0.05). The mRNA and protein expressions of Galectin-3 in Galectin-3-siRNA1 group and Galectin-3-siRNA2 group were significantly lower than those in the control group(P<0.05). The inhibitory effect in Galectin-3-siRNA1 group was more obvious, which was selected for subsequent research. Compared with control group, cell survival rate decreased, Ki-67, PCNA, Notch1, and Hes1 proteins expressions decreased, cell apoptosis rate increased, and the expression of Cleaved Caspase-3 protein increased in Galectin-3-siRNA group(P<0.05).Conclusion:The inhibition of Galectin-3 expression could reduce the cell survival rate, induce apoptosis, and its mechanism may be related to the downregulation of Notch signaling pathway.

参考文献/References:

[1] LERNER SP,BAJORIN DF,DINNEY CP,et al.Summary and recommendations from the national cancer institute's clinical trials planning meeting on novel therapeutics for non-muscle invasive bladder cancer[J].Bladder Cancer,2016,2(2):165
[2] MESQUITA JA,QUEIROZ LM,SILVEIRA EJ,et al.Association of immunoexpression of the galectins-3 and -7 with histopathological and clinical parameters in oral squamous cell carcinoma in young patients[J].Eur Arch Otorhinolaryngol,2016,273(1):237
[3] CAMPO VL,MARCHIORI MF,RODRIGUES LC,et al.Synthetic glycoconjugates inhibitors of tumor-related galectin-3: an update[J].Glycoconj J,2016,33(6):853
[4] ARTIGAS G,HINOU H,GARCIA-MARTIN F,et al.Synthetic mucin-like glycopeptides as versatile tools to measure effects of glycan structure/density/position on the interaction with adhesion/growth-regulatory galectins in arrays[J].Chem Asian J,2017,12(1):159
[5] RABINOVICH GA,CONEJO-GARCIA JR.Shaping the immune landscape in cancer by galectin-driven regulatory pathways[J].J Mol Biol,2016,428(16):3266
[6] DELAINE T,COLLINS P,MACKINNON A,et al.Galectin-3-binding glycomimetics that strongly reduce bleomycin-induced lung fibrosis and modulate intracellular glycan recognition[J].Chembiochem,2016,17(18):1759
[7] 邵世清,杨少琴,黄贵,等.良恶性卵巢甲状腺肿组织中CK19、Galectin-3、CD56蛋白的表达及鉴别诊断价值[J].郑州大学学报(医学版),2015,50(6):847
[8] ANDRE S,KALTNER H,KAYSER K,et al.Merging carbohydrate chemistry with lectin histochemistry to study inhibition of lectin binding by glycoclusters in the natural tissue context[J].Histochem Cell Biol,2016,145(2):185
[9] 李霞,史惠蓉,邓佑兴,等.沉默MACC1的表达对卵巢癌细胞系SKOV-3顺铂化疗敏感性的影响[J].郑州大学学报(医学版),2016,51(1):92
[10]SUNG B,RAVINDRAN J,PRASAD S,et al.Gossypol induces death receptor-5 through activation of ROS-ERK-CHOP pathway and sensitizes colon cancer cells to TRAIL[J].J Biol Chem,2016,291(32):16923
[11]DI KJ,LOMELI N,WOOD SD,et al.Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma[J].Oncotarget,2016,7(47):77457
[12]ABUBAKAR M,HOWAT WJ,DALEY F,et al.High-throughput automated scoring of Ki67 in breast cancer tissue microarrays from the breast cancer association consortium[J].J Pathol Clin Res,2016,2(3):138
[13]李飞,朱彦锋,陈静瑶,等.染料木黄酮对去势抵抗前列腺癌22RV1细胞增殖的影响[J].郑州大学学报(医学版),2017,52(4):393
[14]刘凯,夏进东.MRI联合血清Caspase-3含量检测在卵巢囊腺肿瘤良恶性诊断中的意义[J].河北医学,2016,22(5):753
[15]PRABHU B, SIVAKUMAR A, SUNDARESAN S. Diindolylmethane and lupeol modulates apoptosis and cell proliferation in N-butyl-N-(4-hydroxybutyl)nitrosamine initiated and dimethylarsinic acid promoted rat bladder carcinogenesis[J].Pathol Oncol Res,2016,22(4):747
[16]张瑞瑞,周武碧,潘振国,等.RNA干扰Rac1基因表达抑制胃癌细胞增殖及促进凋亡的机制研究[J].临床和实验医学杂志,2017,16(13):1279
[17]郑介柏,刘旭良,陈文雄,等.DAPT 阻断 Notch信号通路抑制去势大鼠骨髓间充质干细胞增殖及诱导其成骨分化的作用研究[J].河北医学,2015,21(1):9
[18]CUI D,DAI J,KELLER JM,et al.Notch pathway inhibition using PF-03084014,a γ-secretase inhibitor(GSI),enhances the antitumor effect of docetaxel in prostate cancer[J].Clin Cancer Res,2015,21(20):4619
[19]HAYASHI T,GUST KM,WYATT AW,et al.Not all Notch is created equal:the oncogenic role of Notch2 in bladder cancer and its implications for targeted therapy[J].Clin Cancer Res,2016,22(12):2981
[20]PENG DJ,TANIKAWA T,LI W,et al.Myeloid-derived suppressor cells endow stem-like qualities to breast cancer cells through IL6/STAT3 and NO/NOTCH cross-talk signaling[J].Cancer Res,2016,76(11):3156
[21]DELURY C,HART C,BROWN M,et al.Stroma-induced Jagged1 expression drives PC3 prostate cancer cell migration; disparate effects of RIP-generated proteolytic fragments on cell behaviour and Notch signaling[J].Biochem Biophys Res Commun,2016,472(1):255
[22]DOS SANTOS SN,SHELDON H,PEREIRA JX,et al.Galectin-3 acts as an angiogenic switch to induce tumor angiogenesis via Jagged-1/Notch activation[J].Oncotarget,2017,8(30):49484

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备注/Memo

备注/Memo:
【基金项目】河南省科技攻关基金资助项目(132300410018) 【作者简介】张广云,通信作者,男,1983年7月生,硕士,主治医师,研究方向:泌尿系肿瘤,E-mail:icz64b@163.com
更新日期/Last Update: 2019-01-20