[1]王新立,刘汝银,王西彬,等.当归对氧化应激损伤的大鼠髓核细胞增殖和胞外基质合成的影响[J].郑州大学学报(医学版),2019,(01):119-122.[doi:10.13705/j.issn.1671-6825.2018.07.020]
 WANG Xinli,LIU Ruyin,WANG Xibin,et al.Effects of angelica on proliferation and extracellular matrix synthesis in oxidative stress-injured nucleus pulposus cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(01):119-122.[doi:10.13705/j.issn.1671-6825.2018.07.020]
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当归对氧化应激损伤的大鼠髓核细胞增殖和胞外基质合成的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年01期
页码:
119-122
栏目:
应用研究
出版日期:
2019-01-20

文章信息/Info

Title:
Effects of angelica on proliferation and extracellular matrix synthesis in oxidative stress-injured nucleus pulposus cells
作者:
王新立刘汝银王西彬岳宗进
河南省中医院脊柱科 郑州 450000
Author(s):
WANG Xinli LIU Ruyin WANG Xibin YUE Zongjin
Department of Spine Surgery, Henan Province Hospital of Traditional Chinese Medicine, Zhengzhou 450000
关键词:
当归 氧化应激 髓核细胞 增殖 胞外基质 大鼠
Keywords:
angelica oxidative stress nucleus pulposus cell proliferation extracellular matrix rat
分类号:
R917
DOI:
10.13705/j.issn.1671-6825.2018.07.020
摘要:
目的:研究当归对氧化应激损伤的大鼠髓核细胞增殖和胞外基质合成的影响。方法:大鼠髓核细胞分为对照组、H2O2组、H2O2+当归组。H2O2组加入400 μmol/L H2O2刺激6 h; H2O2+当归组先加入1 g/L当归溶液培养24 h,然后加入H2O2刺激6 h; 对照组不进行任何处理。MTT检测细胞活力,Western blot检测增殖相关蛋白[增殖细胞核抗原(PCNA)和细胞周期蛋白(Cyclin)D1],胞外基质相关因子[蛋白聚糖(Aggrecan)、Ⅱ型胶原(Collagen Ⅱ)、基质金属蛋白酶(MMP)3和MMP9]以及Wnt/β-catenin通路相关因子(β-catenin和c-Myc)蛋白的表达,Real-time PCR检测胞外基质相关因子mRNA的表达。结果:与对照组相比,H2O2组细胞活力、PCNA和CyclinD1蛋白的表达、Aggrecan和Collagen Ⅱ mRNA和蛋白的表达均降低,MMP3和MMP9 mRNA与蛋白的表达、β-catenin和c-Myc蛋白的表达均升高; 与H2O2组相比,H2O2+当归组以上指标均有改善(P均<0.05)。结论:当归可能通过抑制Wnt/β-catenin通路,促进氧化应激损伤后髓核细胞的增殖及胞外基质的合成。
Abstract:
Aim:To study the effects and mechanism of angelica on proliferation and extracellular matrix synthesis in oxidative stress-injured nucleus pulposus cells.Methods: Rat nucleus pulposus cells were allocated into control group, H2O2 group, and H2O2+angelica group. Cells in the H2O2 group were treated with 400 μmol/L of H2O2 for 6 hours, in the H2O2+angelica group,with 1 g/L angelica for 24 hours followed by stimulation with H2O2 for 6 hours, and in control group,not treated. MTT assay was used to detect cell viability. Western blot was used to determine the expressions of proliferating-related proteins[proliferating cell nuclear antigen(PCNA)and CyclinD1], extracellular matrix-related proteins[Aggrecan, Collagen Ⅱ, matrix metalloproteinase(MMP)3 and MMP9], and Wnt/β-catenin pathway-related proteins(β-catenin and c-Myc). The mRNA expressions of extracellular matrix-related genes were measured by Real-time PCR.Results:Compared with control group, cell viability, PCNA and CyclinD1 expression, and the mRNA and protein expressions of Aggrecan and Collagen Ⅱ were significantly reduced, whereas the mRNA and protein expressions of MMP3 and MMP9, and expressions of β-catenin and c-Myc protein were notably increased in the H2O2 group(P<0.05). Compared with the H2O2 group, the changes of the above parameters improved remarkably in the H2O2+angelica group(P<0.05).Conclusion:Angelica can prompt proliferation and extracellular matrix synthesis in oxidative stress-injured nucleus pulposus cells via inhibiting Wnt/β-catenin pathway.

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更新日期/Last Update: 2019-01-20