[1]张世甲,李 剑,韩广森.慢病毒介导的FOXJ1过表达对胃腺癌细胞凋亡及线粒体膜电位的影响[J].郑州大学学报(医学版),2019,(02):191-195.[doi:10.13705/j.issn.1671-6825.2018.07.018]
 ZHANG Shijia,LI Jian,HAN Guangsen.Effects of lentivirus mediated FOXJ1 over-expression on cell apoptosis and mitochondrial membrane potential in gastric adenocarcinoma cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(02):191-195.[doi:10.13705/j.issn.1671-6825.2018.07.018]
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慢病毒介导的FOXJ1过表达对胃腺癌细胞凋亡及线粒体膜电位的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年02期
页码:
191-195
栏目:
论著
出版日期:
2019-03-20

文章信息/Info

Title:
Effects of lentivirus mediated FOXJ1 over-expression on cell apoptosis and mitochondrial membrane potential in gastric adenocarcinoma cells
作者:
张世甲李 剑韩广森
郑州大学附属肿瘤医院普外科 郑州 450008
Author(s):
ZHANG ShijiaLI JianHAN Guangsen
Department of General Surgery, Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou 450008
关键词:
胃腺癌 线粒体途径 凋亡 FOXJ1
Keywords:
gastric adenocarcinoma mitochondria pathway apoptosis FOXJ1
分类号:
R735.2
DOI:
10.13705/j.issn.1671-6825.2018.07.018
摘要:
目的:探讨慢病毒介导的叉头框转录因子J1(FOXJ1)过表达对胃腺癌细胞凋亡及线粒体膜电位的影响。方法:人胃腺癌细胞SGC-7901感染FOXJ1过表达慢病毒(Lv-FOXJ1),Real-time PCR和Western blot法检测细胞中FOXJ1 mRNA和蛋白的表达,MTT法检测细胞存活率,细胞克隆实验检测克隆形成率,流式细胞术检测细胞凋亡率,Western blot法检测细胞中激活型Caspase-3(C-Caspase-3)、激活型Caspase-9(C-Caspase-9)和胞浆中细胞色素C(Cyt C)蛋白表达水平,JC-1法检测线粒体膜电位。以感染阴性对照慢病毒和未感染细胞为阴性对照和空白对照。结果:FOXJ1组细胞中FOXJ1 mRNA和蛋白表达水平均增高,细胞存活率、克隆形成率降低,细胞凋亡率升高,细胞中C-Caspase-3、C-Caspase-9蛋白表达水平升高,线粒体膜电位下降,胞浆中Cyt C蛋白表达水平升高(P<0.05)。结论:FOXJ1能够通过线粒体途径诱导胃腺癌细胞凋亡。
Abstract:
Aim:To investigate the effects of lentivirus mediated FOXJ1 over-expression on cell apoptosis and mitochondrial membrane potential in gastric adenocarcinoma cells.Methods:Human gastric adenocarcinoma cell SGC-7901 was infected with FOXJ1 over-expression lentivirus(Lv-FOXJ1),Real-time PCR and Western blot were used to detect the expressions of FOXJ1 mRNA and protein,cell viability was detected by MTT method, cell clone assay was used to detect clone formation ability, cell apoptosis was detected by flow cytometry, Western blot was used to detect the levels of C-Caspase-3 and C-Caspase-9 protein in the cells and Cyt C in the cytoplasm,JC-1 method was used to detect the mitochondrial membrane potential.The cells infected with negative control lentivirus or without infection were the negative control or blank control.Results:Compared with those of the negative control group and blank control group, the expression of FOXJ1 in FOXJ1 group increased,the cell viability and the clone formation ability decreased, the apoptosis rate increased,the levels of C-Caspase-3 and C-Caspase-9 proteins in cells increased, mitochondrial membrane potential dropped, and the level of Cyt C protein in the cytoplasm increased(P<0.05).Conclusion:FOXJ1 can induce apoptosis of gastric adenocarcinoma cells through the mitochondrial pathway.

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备注/Memo

备注/Memo:
【基金项目】河南省科技攻关计划项目(162102310041)
【作者简介】韩广森,通信作者,男,1960年7月生,主任医师,研究方向:消化道、盆腔及软组织肿瘤的诊断和治疗,E-mail:ntlyjn949033w@163.com
更新日期/Last Update: 2019-03-20