[1]陈建安,陈思玉,刘丽文,等.丙酮酸激酶同工酶M2在胆囊癌组织中的表达及其对胆囊癌细胞生物学活性的影响[J].郑州大学学报(医学版),2019,(02):245-249.[doi:10.13705/j.issn.1671-6825.2018.08.041]
 CHEN Jian'an,CHEN Siyu,LIU Liwen,et al.Expression of pyruvate kinase isoenzyme M2 in gallbladder cancer tissue and its biological value on gallbladder cancer cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(02):245-249.[doi:10.13705/j.issn.1671-6825.2018.08.041]
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丙酮酸激酶同工酶M2在胆囊癌组织中的表达及其对胆囊癌细胞生物学活性的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年02期
页码:
245-249
栏目:
应用研究
出版日期:
2019-03-20

文章信息/Info

Title:
Expression of pyruvate kinase isoenzyme M2 in gallbladder cancer tissue and its biological value on gallbladder cancer cells
作者:
陈建安陈思玉刘丽文陈晓龙朱威威余 炎何玉婷孙冉冉任志刚李 娟崔光莹余祖江
郑州大学第一附属医院感染科 郑州 450052
Author(s):
CHEN Jian'anCHEN SiyuLIU LiwenCHEN XiaolongZHU WeiweiYU Yan HE YutingSUN RanranREN ZhigangLI JuanCUI GuangyingYU Zujiang
Department of Infectious Diseases, the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
PKM2 糖酵解 胆囊癌 GBC-SD细胞 NOZ细胞
Keywords:
PKM2 glycolysis gallbladder cancer GBC-SD cell NOZ cell
分类号:
R735.8
DOI:
10.13705/j.issn.1671-6825.2018.08.041
摘要:
目的:探讨丙酮酸激酶同工酶M2(PKM2)在胆囊癌组织中的表达及其对胆囊癌细胞生物学活性的影响。方法:利用免疫组化法检测胆囊癌和癌旁正常组织中PKM2蛋白的表达。采用siRNA转染试剂将PKM2 siRNA和阴性对照SiRNA转染入胆囊癌GBC-SD细胞和NOZ细胞中,使用细胞免疫荧光和Western blot法检测两种胆囊癌细胞中PKM2蛋白的表达,应用MTT和Transwell实验检测细胞增殖、侵袭能力,使用葡萄糖、乳酸和ATP检测试剂盒检测细胞糖代谢能力。结果:PKM2蛋白在胆囊癌组织中的表达水平高于癌旁正常组织(P<0.001)。PKM2蛋白主要分布于GBC-SD和NOZ细胞的细胞质区域。转染PKM2 siRNA后,胆囊癌GBC-SD和NOZ细胞中PKM2的表达水平降低,细胞增殖、侵袭能力均降低(P<0.05),同时葡萄糖消耗量、细胞外乳酸生成量和ATP消耗量均明显减少(P<0.05)。结论:PKM2在胆囊癌组织和细胞中高表达,其与胆囊癌细胞的增殖、侵袭能力关系密切,有望成为一个新的治疗胆囊癌的靶点。
Abstract:
Aim:To investigate the expression of pyruvate kinase isoenzyme M2(PKM2)in gallbladder cancer(GBC)tissue and its effects on the biological activity of GBC cells.Methods:Immunohistochemical staining was used to detect the expression of PKM2 protein in GBC tissue and adjacent normal tissue. PKM2 siRNA and negative control siRNA were transfected into GBC-SD cell and NOZ cells by siRNA transfection reagent. The expression of PKM2 protein in 2 kinds of GBC cells was detected by immunofluorescence and Western blot. MTT and Transwell assays were used to detect the changes of cell proliferation and invasion abilities, and glucose, lactic acid and ATP kits were used to determine the changes of glucose metabolism.Results:The expression level of PKM2 protein in GBC tissue was higher than that in adjacent normal tissue(P<0.001). PKM2 was mainly distributed in the cytoplasmic region of GBC-SD and NOZ cells. After transfection of PKM2 siRNA, the expression level of PKM2 decreased in GBC-SD and NOZ cells, and the cells proliferation and invasion abilities were decreased(P<0.05). Meanwhile, the levels of glucose consumption, extracellular lactic acid production and ATP consumption were significantly reduced(P<0.05).Conclusion:PKM2 is highly expressed in GBC tissue and cells, which is closely related to the proliferation and invasion abilities of GBC cells, and is expected to be a novel target for the treatment of GBC.

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备注/Memo

备注/Memo:
【基金项目】传染病诊治国家科技重大专项(SQ2018ZX100301); 国家自然科学基金青年基金(81702346, 81702927, 81702757, 81600506)
【作者简介】余祖江,通信作者,男,1971年5月生,博士,教授,博士研究生导师,研究方向:重症肝炎和肝癌的综合治疗,E-mail:johnyuem@zzu.edu.cn
更新日期/Last Update: 2019-03-20