[1]司 文),郭 壮),周 华).黄芪多糖联合Smac shRNA对高糖诱导的血管内皮细胞凋亡的影响[J].郑州大学学报(医学版),2019,(02):254-257.[doi:10.13705/j.issn.1671-6825.2018.10.114]
 SI Wen),GUO Zhuang),ZHOU Hua).Effects of astragalus polysaccharide combined with Smac shRNA on apoptosis of vascular endothelial cells induced by high glucose[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(02):254-257.[doi:10.13705/j.issn.1671-6825.2018.10.114]
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黄芪多糖联合Smac shRNA对高糖诱导的血管内皮细胞凋亡的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年02期
页码:
254-257
栏目:
应用研究
出版日期:
2019-03-20

文章信息/Info

Title:
Effects of astragalus polysaccharide combined with Smac shRNA on apoptosis of vascular endothelial cells induced by high glucose
作者:
司 文1)郭 壮2)周 华3)
1)胜利油田中心医院内分泌科 山东东营 257034 2)胜利油田中心医院消化内科 山东东营 257034 3)山东大学附属省立医院血管外科 济南250021
Author(s):
SI Wen1) GUO Zhuang2) ZHOU Hua3)
1)Department of Endocrinology, Shengli Oil Field Central Hospital, Dongying, Shandong 257034 2)Department of Gastroenterology, Shengli Oil Field Central Hospital,Dongying, Shandong 257034 3)Department of Vascular Surgery,the Provincial Hospital Affiliated to Shandong University,Jinan 250021
关键词:
高糖 血管内皮细胞 黄芪多糖 Smac 凋亡
Keywords:
high glucose vascular endothelial cell astragalus polysaccharide Smac apoptosis
分类号:
R587.1
DOI:
10.13705/j.issn.1671-6825.2018.10.114
摘要:
目的:探讨黄芪多糖联合Smac shRNA对高糖诱导的血管内皮细胞凋亡的影响。方法:以Smac shRNA慢病毒感染血管内皮细胞EVC-304,用Real-time PCR和Western blot法测定干扰效果。EVC-304细胞分为4组,对照组未处理,黄芪多糖组给予300 mg/L黄芪多糖,干扰组感染Smac shRNA慢病毒, 黄芪多糖+干扰组用300 mg/L黄芪多糖培养感染Smac shRNA慢病毒的EVC-304细胞,随后4组均给予高糖处理。流式细胞术测定细胞凋亡,生化试剂盒测定培养液中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量,Western blot法检测细胞激活型Caspase-3(C-Caspase-3)、C-Caspase-9蛋白的表达水平。结果:感染Smac shRNA慢病毒的EVC-304细胞Smac表达水平降低(P<0.05)。Smac shRNA干扰或黄芪多糖处理均可降低高糖条件下EVC-304细胞凋亡率,提高SOD和CAT活性,减少MDA含量,抑制细胞中C-Caspase-3、C-Caspase-9蛋白表达,二者联用有协同作用(P<0.05)。结论:黄芪多糖联合Smac干扰可抑制高糖诱导的血管内皮细胞凋亡,其作用机制可能与提高抗氧化酶活性、减少氧化损伤有关。
Abstract:
Aim:To investigate the effects of astragalus polysaccharide combined with Smac shRNA on apoptosis of vascular endothelial cells induced by high glucose.Methods:EVC-304 cells were infected with Smac shRNA lentivirus, and the interference effect was determined by Real-time PCR and Western blot. EVC-304 cells were divided into four groups,the control group was not treated, the astragalus polysaccharide group was treated with 300 mg/L astragalus polysaccharide, the interfering group was infected with Smac shRNA lentivirus, and the astragalus polysaccharide+interfering group was treated with 300 mg/L astragalus polysaccharide and infected with Smac shRNA, and then the four groups were cultured with high sugar.Results:Smac expression in EVC-304 cells infected with Smac shRNA lentivirus decreased(P<0.05). Smac shRNA interference or astragalus polysaccharide treatment could reduce the apoptotic rate of EVC-304 cells, increase SOD and CAT activity, reduce MDA content, and inhibit the expressions of C-Caspase-3 and C-Caspase-9 protein in cells under high glucose conditions, and the combination of them had synergistic effects(P<0.05).Conclusion:Astragalus polysaccharide combined with Smac shRNA could inhibit the apoptosis of vascular endothelial cells induced by high glucose,and the mechanism may be related with enhancing antioxidant enzyme activity and reducing oxidative damage.

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备注/Memo

备注/Memo:
【基金项目】山东省自然科学基金资助项目(ZR2016HL096)
【作者简介】司文,通信作者,女,1986年9月生,硕士研究生,主治医师,研究方向:内分泌与代谢性疾病,E-mail:siwen20112018@163.com
更新日期/Last Update: 2019-03-20