[1]郝晓慧),赵明中),张玉芝),等.过表达E1A激活基因阻遏子对OxLDL诱导的血管内皮细胞损伤的影响[J].郑州大学学报(医学版),2019,(02):258-262.[doi:10.13705/j.issn.1671-6825.2018.08.110]
 HAO Xiaohui),ZHAO Mingzhong),ZHANG Yuzhi),et al.Effects of overexpression of cellular repressor of E1A-stimulated gene on vascular endothelial cell injury induced by OxLDL[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(02):258-262.[doi:10.13705/j.issn.1671-6825.2018.08.110]
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过表达E1A激活基因阻遏子对OxLDL诱导的血管内皮细胞损伤的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2019年02期
页码:
258-262
栏目:
应用研究
出版日期:
2019-03-20

文章信息/Info

Title:
Effects of overexpression of cellular repressor of E1A-stimulated gene on vascular endothelial cell injury induced by OxLDL
作者:
郝晓慧1)赵明中1)张玉芝1)张祖峰1)朱秋平1)牛方卿2)
1)郑州市第九人民医院心脏中心 郑州 450053 2)阜外华中心血管病医院心内科 郑州 450000
Author(s):
HAO Xiaohui1) ZHAO Mingzhong1) ZHANG Yuzhi1) ZHANG Zufeng1) ZHU Qiuping1)NIU Fangqing2)
1)Heart Center,the Ninth People's Hospital of Zhengzhou, Zhengzhou 450053 2)Department of Cardiology,Fuwai Central China Cardiovascular Hospital,Zhengzhou 450000
关键词:
血管内皮细胞 凋亡 E1A激活基因阻遏子 OxLDL
Keywords:
vascular endothelial cell apoptosis cellular repressor of E1A-stimulated gene OxLDL
分类号:
R541.4
DOI:
10.13705/j.issn.1671-6825.2018.08.110
摘要:
目的:研究过表达E1A激活基因阻遏子(CREG)对氧化低密度脂蛋白(OxLDL)诱导的血管内皮细胞损伤的影响。方法:血管内皮细胞分为4组,空白对照组不处理; OxLDL组用100 mg/L OxLDL处理24 h; 另2组分别感染对照逆转录病毒(阴性对照组)和pLNCX-CREG重组逆转录病毒(pLNCX-CREG组),之后均用100 mg/L OxLDL处理24 h。用Real-time PCR和Western blot法检测4组细胞CREG mRNA和蛋白的表达,用硫代巴比妥酸法检测培养液中丙二醛(MDA)含量,黄嘌呤氧化酶法检测培养液中超氧化物歧化酶(SOD)活性,DCFH-DA法检测培养液中活性氧(ROS)水平,流式细胞术检测细胞凋亡,Western blot法检测细胞中活化的Caspase-3(Cleaved Caspase-3)蛋白的表达水平。结果:与空白对照组比较,OxLDL组细胞中CREG mRNA和蛋白表达水平均降低; pLNCX-CREG组细胞中CREG mRNA和蛋白表达水平均较OxLDL组升高(P<0.05)。与空白对照组比较,OxLDL组细胞SOD活性降低,MDA和ROS水平升高,细胞凋亡率和Cleaved Caspase-3蛋白表达水平均升高; 与OxLDL组比较,pLNCX-CREG组细胞氧化损伤指标的变化被逆转,细胞凋亡率及Cleaved Caspase-3蛋白表达水平均降低(P<0.05)。结论:过表达CREG可以抑制OxLDL诱导的血管内皮细胞氧化损伤并抑制细胞凋亡。
Abstract:
Aim:To study the effects of cellular repressor of E1A-stimulated gene(CREG)overexpression on vascular endothelial cell injury induced by OxLDL.Methods:Vascular endothelial cells were divided into four groups, the blank control group was not treated, the OxLDL group was treated with 100 mg/L OxLDL for 24 hours, the other two groups were infected with control retrovirus(negative control group)and pLNCX-CREG retrovirus(pLNCX-CREG group), and then treated with 100 mg/L OxLDL for 24 hours. The expression of CREG was detected by Real-time PCR and Western blot. The content of malondialdehyde(MDA)in culture fluid was detected by thiobarbituric acid, the activity of superoxide dismutase(SOD)was detected by xanthine oxidase,and the level of reactive oxygen species(ROS)was detected by DCFH-DA.The apoptosis was detected by flow cytometry, and the activated Caspase-3(Cleaved Caspase-3)was detected by Western blot.Results:Compared with the blank control group, the expression levels of CREG mRNA and protein in the OxLDL group were decreased, while those in the pLNCX-CREG group were increased compared with the OxLDL group(P<0.05). Compared with the blank control group, SOD activity decreased, MDA and ROS levels increased, apoptotic rate and Cleaved Caspase-3 protein expression increased in the OxLDL group. Compared with the OxLDL group, the changes of oxidative damage indexes in the pLNCX-CREG group were reversed, apoptotic rate and expression of Cleaved Caspase-3 protein were decreased(P<0.05).Conclusion:Overexpression of CREG can inhibit oxidative damage and reduce apoptosis of vascular endothelial cells induced by OxLDL.

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备注/Memo

备注/Memo:
【基金项目】河南省医学科技攻关计划项目(201504065)
【作者简介】赵明中,通信作者,男,1963年3月生,博士,主任医师,教授,研究方向:介入心脏病学,心脏康复,E-mail:olg550635@163.com
更新日期/Last Update: 2019-03-20